PMID- 37734580
OWN - NLM
STAT- MEDLINE
DCOM- 20231108
LR  - 20231108
IS  - 1096-0953 (Electronic)
IS  - 0013-9351 (Linking)
VI  - 238
IP  - Pt 2
DP  - 2023 Dec 1
TI  - Development of a high-performance label-free electrochemical immunosensor for 
      early cancer diagnosis using anti-CEA/Ag-MOF/GO/GCE nanocomposite.
PG  - 117178
LID - S0013-9351(23)01982-5 [pii]
LID - 10.1016/j.envres.2023.117178 [doi]
AB  - In order to detect carcinoembryonic antigen (CEA) as a tumor marker in lung 
      cancer for early cancer diagnosis, this study aimed to develop a label-free 
      electrochemical immunosensor based on the immobilization of an Anti-CEA antibody 
      on a metal-organic framework (MOF)-graphene oxide nanocomposite modified glassy 
      carbon electrode (Anti-CEA/Ag-MOF/GO/GCE). Ag-MOF/GO nanocomposite was prepared 
      on the GCE surface using the ultrasonic irradiation method, and Anti-CEA antibody 
      was subsequently immobilized on the surface. Analysis of the crystal structure 
      and morphology of the modified electrode using FE-SEM and XRD revealed that the 
      correct combination of GO nanosheets and Ag-MOF nanoparticles produced a high 
      surface area to trap the antibodies. Electrochemical tests utilizing the CV and 
      DPV methods revealed that the immunosensor's sensitivity, stability, and 
      selectivity were improved by Anti-CEA/Ag-MOF/GO/GCE. Results showed that, with a 
      detection limit of 0.005 ng/mL, the change in the reduction peak current was 
      inversely correlated with the logarithm concentration of CEA in the range of 10-3 
      to 5000 ng/mL. The suggested CEA immunosensor's applicability in a human serum 
      sample was investigated, and findings of analytical studies via standard addition 
      technique for both ELISA and DPV assays revealed that significant agreement 
      existed between the outcomes of the two assays. Additionally, the recoveries 
      ranged from 99.00% to 99.25%, and all relative standard deviations (RSDs) for the 
      sample detections were below 5.01%, indicating satisfactory accuracy in results 
      measured with the proposed CEA immunosensor, indicating that the prepared CEA 
      immunosensor in this study can be used in clinical applications and human fluids.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Huang, Peng
AU  - Huang P
AD  - Department of Nephrology, Affiliated Hospital of Youjiang Medical University for 
      Nationalities, Guangxi, 533000, China.
FAU - Meng, Lingzhang
AU  - Meng L
AD  - Center for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, 
      Youjiang Medical University for Nationalities, Guangxi, 533000, China.
FAU - Pang, Jun
AU  - Pang J
AD  - Department of Nephrology, Affiliated Hospital of Youjiang Medical University for 
      Nationalities, Guangxi, 533000, China.
FAU - Huang, Haiting
AU  - Huang H
AD  - Department of Nephrology, Affiliated Hospital of Youjiang Medical University for 
      Nationalities, Guangxi, 533000, China.
FAU - Ma, Jing
AU  - Ma J
AD  - Department of Nephrology, Affiliated Hospital of Youjiang Medical University for 
      Nationalities, Guangxi, 533000, China.
FAU - He, Linlin
AU  - He L
AD  - Department of Nephrology, Affiliated Hospital of Youjiang Medical University for 
      Nationalities, Guangxi, 533000, China.
FAU - Amani, Parnian
AU  - Amani P
AD  - Department of Molecular and Cell Biology, Faculty of Basic Sciences, University 
      of Mazandaran, Babolsar, Iran. Electronic address: Parnianamanii1@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230920
PL  - Netherlands
TA  - Environ Res
JT  - Environmental research
JID - 0147621
RN  - 0 (Metal-Organic Frameworks)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Humans
MH  - *Metal-Organic Frameworks
MH  - Carcinoembryonic Antigen/analysis
MH  - *Biosensing Techniques/methods
MH  - Immunoassay/methods
MH  - *Nanocomposites/chemistry
MH  - *Metal Nanoparticles/chemistry
MH  - *Neoplasms
MH  - Gold/chemistry
MH  - Limit of Detection
OTO - NOTNLM
OT  - Ag-MOF
OT  - Carcinoembryonic antigen
OT  - Electrochemical immunosensor
OT  - Graphene oxide
OT  - Nanocomposite
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/22 00:42
MHDA- 2023/11/08 06:43
CRDT- 2023/09/21 19:16
PHST- 2023/07/31 00:00 [received]
PHST- 2023/09/07 00:00 [revised]
PHST- 2023/09/19 00:00 [accepted]
PHST- 2023/11/08 06:43 [medline]
PHST- 2023/09/22 00:42 [pubmed]
PHST- 2023/09/21 19:16 [entrez]
AID - S0013-9351(23)01982-5 [pii]
AID - 10.1016/j.envres.2023.117178 [doi]
PST - ppublish
SO  - Environ Res. 2023 Dec 1;238(Pt 2):117178. doi: 10.1016/j.envres.2023.117178. Epub 
      2023 Sep 20.