PMID- 37736814
OWN - NLM
STAT- MEDLINE
DCOM- 20231204
LR  - 20231217
IS  - 1438-2199 (Electronic)
IS  - 0939-4451 (Print)
IS  - 0939-4451 (Linking)
VI  - 55
IP  - 11
DP  - 2023 Nov
TI  - Metabolomic profiling of amino acids study reveals a distinct diagnostic model 
      for diabetic kidney disease.
PG  - 1563-1572
LID - 10.1007/s00726-023-03330-0 [doi]
AB  - Diabetic kidney disease (DKD), a highly prevalent complication of diabetes 
      mellitus, is a major cause of mortality in patients. However, identifying 
      circulatory markers to diagnose DKD requires a thorough understanding of the 
      metabolic mechanisms of DKD. In this study, we performed ultra-performance liquid 
      chromatography tandem mass spectrometry (UPLC-MS/MS) to reveal altered metabolic 
      profiles of amino acids (AAs) in patients with DKD. We found decreased plasma 
      levels of histidine and valine, increased urine levels of proline, decreased 
      urine levels of histidine and valine, and increased saliva levels of arginine in 
      patients with DKD compared with the levels in patients with type 2 diabetes 
      mellitus (T2DM) and in healthy controls. Our analyses of the key metabolites and 
      metabolic enzymes involved in histidine and valine metabolism indicated that the 
      AAs level alterations may be due to enhanced carnosine hydrolysis, decreased 
      degradation of homocarnosine and anserine, enhanced histidine methylation, and 
      systemic enhancement of valine metabolism in patients with DKD. Notably, we 
      generated a distinct diagnostic model with an AUC of 0.957 and an accuracy up to 
      92.2% on the basis of the AA profiles in plasma, urine and saliva differing in 
      patients with DKD using logistic regression and receiver operating characteristic 
      analyses. In conclusion, our results suggest that altered AA metabolic profiles 
      are associated with the progression of DKD. Our DKD diagnostic model on the basis 
      of AA levels in plasma, urine, and saliva may provide a theoretical basis for 
      innovative strategies to diagnose DKD that may replace cumbersome kidney 
      biopsies.
CI  - (c) 2023. The Author(s).
FAU - Wang, Jiao
AU  - Wang J
AD  - Department of Geriatric Endocrinology, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450000, China.
AD  - Research Institute of Nephrology, Zhengzhou University, Zhengzhou, 450000, China.
AD  - Henan Province Research Center For Kidney Disease, Zhengzhou, 450000, China.
FAU - Zhou, Chunyu
AU  - Zhou C
AD  - Research Institute of Nephrology, Zhengzhou University, Zhengzhou, 450000, China.
AD  - Henan Province Research Center For Kidney Disease, Zhengzhou, 450000, China.
AD  - Blood Purification Center, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, 450000, China.
FAU - Zhang, Qing
AU  - Zhang Q
AD  - Research Institute of Nephrology, Zhengzhou University, Zhengzhou, 450000, China. 
      aquamarine61@163.com.
AD  - Henan Province Research Center For Kidney Disease, Zhengzhou, 450000, China. 
      aquamarine61@163.com.
FAU - Liu, Zhangsuo
AU  - Liu Z
AD  - Research Institute of Nephrology, Zhengzhou University, Zhengzhou, 450000, China. 
      zhangsuoliu@zzu.edu.cn.
AD  - Henan Province Research Center For Kidney Disease, Zhengzhou, 450000, China. 
      zhangsuoliu@zzu.edu.cn.
AD  - Blood Purification Center, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, 450000, China. zhangsuoliu@zzu.edu.cn.
AD  - Traditional Chinese Medicine Integrated Department of Nephrology, The First 
      Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China. 
      zhangsuoliu@zzu.edu.cn.
LA  - eng
GR  - 222300420345/Natural Science Foundation of Henan Province/
GR  - 222300420343/Natural Science Foundation of Henan Province/
GR  - LHGJ20200328/Henan Medical Science and Technology Research Program Joint 
      Construction Project/
GR  - LHGJ20200290/Henan Medical Science and Technology Research Program Joint 
      Construction Project/
GR  - SBGJ202103080/Henan Medical Science and Technique Foundation/
PT  - Journal Article
DEP - 20230922
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - 0 (Amino Acids)
RN  - 4QD397987E (Histidine)
RN  - 0 (Amines)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Humans
MH  - *Diabetic Nephropathies/metabolism
MH  - Amino Acids
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Histidine
MH  - Chromatography, Liquid
MH  - Tandem Mass Spectrometry
MH  - Amines
MH  - Valine
PMC - PMC10689543
OTO - NOTNLM
OT  - Amino acids
OT  - Diabetic kidney disease
OT  - Diagnostic model
OT  - LC-MS/MS
OT  - Metabolomics
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/09/22 12:43
MHDA- 2023/12/04 12:42
PMCR- 2023/09/22
CRDT- 2023/09/22 06:28
PHST- 2023/07/17 00:00 [received]
PHST- 2023/08/30 00:00 [accepted]
PHST- 2023/12/04 12:42 [medline]
PHST- 2023/09/22 12:43 [pubmed]
PHST- 2023/09/22 06:28 [entrez]
PHST- 2023/09/22 00:00 [pmc-release]
AID - 10.1007/s00726-023-03330-0 [pii]
AID - 3330 [pii]
AID - 10.1007/s00726-023-03330-0 [doi]
PST - ppublish
SO  - Amino Acids. 2023 Nov;55(11):1563-1572. doi: 10.1007/s00726-023-03330-0. Epub 
      2023 Sep 22.