PMID- 37737984
OWN - NLM
STAT- MEDLINE
DCOM- 20230925
LR  - 20230926
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2717
DP  - 2024
TI  - Coculture of Human Dendritic and T Cells for the Study of Specific T 
      Cell-Mediated Responses Against Food Allergens.
PG  - 175-190
LID - 10.1007/978-1-0716-3453-0_11 [doi]
AB  - Dendritic cells (DCs) connect innate and adaptive immunity by sampling, 
      capturing, processing, and presenting the allergen to distinct subsets of CD4(+) 
      T cells. In food allergy, this process leads to the generation of 
      allergen-specific Th2 responses and the production of type 2 cytokines that 
      ultimately induce the synthesis of IgE by allergen-specific B cells. In this 
      chapter, we have described different protocols for the isolation of circulating 
      DCs as well as the generation of DC-like cells derived from autologous peripheral 
      monocytes and the human monocytic THP-1 cell line. Coculture of 
      isolated/generated DCs with CD4(+) T cells obtained from PBMCs of allergic 
      subjects allows the study of antigen-specific T cell immune responses against 
      food allergens. Early responses upon allergen recognition can be determined by 
      the upregulation of activation markers such as CD154 (CD40 ligand) and the 
      detection of type 2 cytokines (IL-4, IL-5, IL-9, and IL-13). Delayed 
      allergen-specific CD4(+) T cell responses induce the proliferation of these cells 
      and the accumulation of type 2 cytokines in coculture supernatants that can be 
      quantified by different approaches (ELISA, Ella(TM), and multiplex assays). 
      Together, the protocols described in this chapter can be used to investigate the 
      features of food proteins to induce food allergy, the influence of environmental 
      factors to generate Th2-polarization, the function of DCs to generate 
      differential immune responses in allergic versus tolerant individuals, and to 
      assess the immunomodulating properties of potential therapeutic substances.
CI  - (c) 2024. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Martinez-Blanco, Monica
AU  - Martinez-Blanco M
AD  - Division of Immunology, Boston Children's Hospital, Boston, MA, USA.
AD  - Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
FAU - Menchen-Martinez, David
AU  - Menchen-Martinez D
AD  - Instituto de Investigacion en Ciencias de la Alimentacion (CIAL, CSIC-UAM), 
      Madrid, Spain.
FAU - Camara, Carmen
AU  - Camara C
AD  - Department of Immunology, Hospital La Paz, Madrid, Spain.
FAU - Lopez-Fandino, Rosina
AU  - Lopez-Fandino R
AD  - Instituto de Investigacion en Ciencias de la Alimentacion (CIAL, CSIC-UAM), 
      Madrid, Spain.
FAU - Berin, M Cecilia
AU  - Berin MC
AD  - Department of Medicine, Feinberg School of Medicine at Northwestern University, 
      Chicago, IL, USA.
FAU - Lozano-Ojalvo, Daniel
AU  - Lozano-Ojalvo D
AD  - Instituto de Investigacion en Ciencias de la Alimentacion (CIAL, CSIC-UAM), 
      Madrid, Spain. daniel.lozano-ojalvo@mssm.edu.
AD  - Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, 
      USA. daniel.lozano-ojalvo@mssm.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cytokines)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 0 (Allergens)
SB  - IM
MH  - Humans
MH  - Coculture Techniques
MH  - *T-Lymphocytes
MH  - *Food Hypersensitivity
MH  - Cytokines
MH  - CD40 Ligand
MH  - Allergens
OTO - NOTNLM
OT  - Allergen specific
OT  - Cytokine
OT  - Dendritic cells
OT  - Food allergy
OT  - In vitro
OT  - T cells
EDAT- 2023/09/22 12:42
MHDA- 2023/09/25 06:42
CRDT- 2023/09/22 11:09
PHST- 2023/09/25 06:42 [medline]
PHST- 2023/09/22 12:42 [pubmed]
PHST- 2023/09/22 11:09 [entrez]
AID - 10.1007/978-1-0716-3453-0_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2024;2717:175-190. doi: 10.1007/978-1-0716-3453-0_11.