PMID- 37738917
OWN - NLM
STAT- Publisher
LR  - 20231022
IS  - 1532-8511 (Electronic)
IS  - 1052-3057 (Linking)
VI  - 32
IP  - 11
DP  - 2023 Nov
TI  - Bone marrow mesenchymal stem cells-derived exosomes mediated delivery of 
      tetramethylpyrazine attenuate cerebral ischemic injury.
PG  - 107369
LID - S1052-3057(23)00392-0 [pii]
LID - 10.1016/j.jstrokecerebrovasdis.2023.107369 [doi]
AB  - OBJECTIVES: Tetramethylpyrazine (TEP) can protect the brain from ischemic damage, 
      but it has defects such as short half-life, fast absorption, wide distribution, 
      and rapid elimination, which limits its application. Exosomes (Exos) have the 
      property of loading drugs and transporting signal substances. Here, we elucidated 
      the effect of TEP-loaded bone marrow mesenchymal stem cell (BMSC)-derived Exos 
      (Exo-TEP) on cerebral ischemic injury. MATERIALS AND METHODS: The Exos were 
      extracted by ultracentrifugation and TEP was loaded into the Exos by 
      electroporation. Oxygen-glucose deprivation (OGD) induced-primary cortical 
      neurons and middle cerebral artery occlusion (MCAO)-induced mouse models were 
      used to determine the effect of Exo-TEP on cerebral ischemic injury in vitro and 
      in vivo. RESULTS: Exo-TEP exhibited a stable and sustained release pattern 
      compared to free TEP. Exo-TEP treatment was more significant in improving 
      OGD-mediated decrease in cell activity, as well as a elevation in apoptosis and 
      ROS production in cortical neurons. In comparison with Exo and free TEP 
      treatment, Exo-TEP treatment significantly improved pathological changes, shrunk 
      cerebral infarction volume, as well reduced neurological deficit scores and 
      neuronal apoptosis, and oxidative stress. CONCLUSIONS: Exo-TEP was superior to 
      free TEP in improving cerebral ischemic injury by reducing neuronal apoptosis and 
      oxidative stress.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Zhang, Guozhen
AU  - Zhang G
AD  - Neurosurgery Department, Dongzhimen Hospital Beijing University of Chinese 
      Medicine, Beijing, 100700, China. Electronic address: guozhen9119@163.com.
FAU - Xu, Bin
AU  - Xu B
AD  - Neurosurgery Department, Dongzhimen Hospital Beijing University of Chinese 
      Medicine, Beijing, 100700, China.
FAU - Mao, Jinlong
AU  - Mao J
AD  - Neurosurgery Department, the Chinese PLA General Hospital 7th Medical Center, 
      Beijing, 100000, China.
FAU - Liu, Ruicun
AU  - Liu R
AD  - Neurosurgery Department, Tsinghua University Yuquan Hospital, Beijing, 100000, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20230920
PL  - United States
TA  - J Stroke Cerebrovasc Dis
JT  - Journal of stroke and cerebrovascular diseases : the official journal of National 
      Stroke Association
JID - 9111633
SB  - IM
OTO - NOTNLM
OT  - Apoptosis
OT  - Bone marrow mesenchymal stem cells
OT  - Cerebral ischemic
OT  - Exosomes
OT  - Tetramethylpyrazine
COIS- Declaration of Competing Interest The authors declare that they have no competing 
      interests.
EDAT- 2023/09/23 11:42
MHDA- 2023/09/23 11:42
CRDT- 2023/09/22 18:10
PHST- 2023/07/30 00:00 [received]
PHST- 2023/08/21 00:00 [revised]
PHST- 2023/09/12 00:00 [accepted]
PHST- 2023/09/23 11:42 [pubmed]
PHST- 2023/09/23 11:42 [medline]
PHST- 2023/09/22 18:10 [entrez]
AID - S1052-3057(23)00392-0 [pii]
AID - 10.1016/j.jstrokecerebrovasdis.2023.107369 [doi]
PST - ppublish
SO  - J Stroke Cerebrovasc Dis. 2023 Nov;32(11):107369. doi: 
      10.1016/j.jstrokecerebrovasdis.2023.107369. Epub 2023 Sep 20.