PMID- 37740615
OWN - NLM
STAT- MEDLINE
DCOM- 20231204
LR  - 20231217
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 52
IP  - 1
DP  - 2024 Jan
TI  - Immunoreactivity of TTF-1, GATA-3, CEA, and p16/Ki67 cocktail in 
      Cellprep(R)-processed control samples: Comparison of long-term storage in vials and 
      slides.
PG  - 3-9
LID - 10.1002/dc.25226 [doi]
AB  - INTRODUCTION: Cellprep(R) (CP) is a novel liquid-based cytology (LBC) system. This 
      study aimed to assess the immunoreactivity of control samples stored long-term 
      under two storage conditions using CP. METHODS: The immunoreactivity in control 
      samples was evaluated under two sample storage conditions: CP vial storage at 
      room temperature (RT: 20-25 degrees C) and CP slide storage in a refrigerator (2-6 degrees C). 
      Clinical samples as controls (total: positive/negative) were immunostained using 
      thyroid transcription factor-1 (TTF-1) (20: 14/6), GATA binding protein 3 
      (GATA-3) (13: 10/3), carcinoembryonic antigen (CEA) (23: 15/8), and the p16/Ki67 
      cocktail (20: 12/8) markers. The first immunocytochemistry (ICC) was performed 
      within 1 month using CP vials stored at RT. Samples stored in CP vials and on CP 
      slides were used for the second (within 3-6 months) and third (within 
      6-11 months) ICCs. Compared with the first ICC, the concordance of 
      immunoreactivity for the second and third ICCs was evaluated using the weighted 
      kappa coefficient. RESULTS: For TTF-1, CEA, and the p16/Ki67 cocktail markers, 
      ICC controls had stable immunoreactivity for a minimum of 6 months when samples 
      were stored in CP vials (kappa coefficients >0.8), whereas for GATA-3, they were 
      for 3 months. On CP slides, only for the p16/Ki67 cocktail, ICC controls had 
      stable immunoreactivity for at least 3 months (kappa coefficient >0.8). 
      CONCLUSION: Clinical samples as ICC controls revealed consistently more stable 
      immunoreactivity in CP vials than on CP slides for TTF-1, GATA-3, CEA, and the 
      p16/Ki67 cocktail markers.
CI  - (c) 2023 Wiley Periodicals LLC.
FAU - Ryu, Ayumi
AU  - Ryu A
AUID- ORCID: 0000-0002-7834-5223
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
AD  - Department of Oncology, Graduate School of Medicine, Osaka University, Osaka, 
      Japan.
FAU - Honma, Keiichiro
AU  - Honma K
AUID- ORCID: 0000-0001-5193-5891
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
FAU - Umeno, Yoshimi
AU  - Umeno Y
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
FAU - Uefuji, Fusayo
AU  - Uefuji F
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
FAU - Muramatsu, Takako
AU  - Muramatsu T
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
FAU - Nishimura, Sanako
AU  - Nishimura S
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
FAU - Tanada, Satoshi
AU  - Tanada S
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
FAU - Yamamoto, Takashi
AU  - Yamamoto T
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
FAU - Nagata, Shigenori
AU  - Nagata S
AUID- ORCID: 0000-0002-5455-6886
AD  - Department of Diagnostic Pathology and Cytology, Osaka International Cancer 
      Institute, Osaka, Japan.
FAU - Yamasaki, Tomoyuki
AU  - Yamasaki T
AD  - Department of Clinical Laboratory, Osaka International Cancer Institute, Osaka, 
      Japan.
FAU - Ohue, Masayuki
AU  - Ohue M
AD  - Department of Oncology, Graduate School of Medicine, Osaka University, Osaka, 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20230923
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Humans
MH  - *Carcinoembryonic Antigen
MH  - Ki-67 Antigen
MH  - *Cytodiagnosis
OTO - NOTNLM
OT  - Cellprep
OT  - control
OT  - immunocytochemistry
OT  - liquid-based cytology
OT  - storage
EDAT- 2023/09/23 11:43
MHDA- 2023/12/04 12:43
CRDT- 2023/09/23 04:44
PHST- 2023/09/01 00:00 [revised]
PHST- 2023/06/24 00:00 [received]
PHST- 2023/09/11 00:00 [accepted]
PHST- 2023/12/04 12:43 [medline]
PHST- 2023/09/23 11:43 [pubmed]
PHST- 2023/09/23 04:44 [entrez]
AID - 10.1002/dc.25226 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2024 Jan;52(1):3-9. doi: 10.1002/dc.25226. Epub 2023 Sep 23.