PMID- 37740638
OWN - NLM
STAT- MEDLINE
DCOM- 20231102
LR  - 20231102
IS  - 1756-185X (Electronic)
IS  - 1756-1841 (Linking)
VI  - 26
IP  - 11
DP  - 2023 Nov
TI  - Genome-wide DNA methylation sequencing reveals epigenetic features and potential 
      biomarkers of Sjogren syndrome.
PG  - 2223-2232
LID - 10.1111/1756-185X.14918 [doi]
AB  - AIM: Sjogren syndrome (SS) is a slowly progressive, inflammatory, autoimmune 
      disease. The aim of this study was to construct the DNA methylation profiles of 
      whole blood of SS patients and healthy controls (HC), and to explore the role of 
      differentially methylated genes in the pathogenesis of the disease. METHODS: 
      Whole-genome bisulfite sequencing was performed on three SS patients and four HC. 
      The biological function of genes associated with differentially methylated 
      regions (DMRs) was investigated using Gene Ontology functional analysis and Kyoto 
      Encyclopedia of Genes and Genomes pathway analysis, using network-based key 
      driver analysis (KDA) to find KDA genes. In clinical samples of SS patients and 
      controls, the expression levels of KDA genes were validated by quantitative 
      real-time polymerase chain reaction and immunohistochemical analysis. Moreover, 
      the diagnostic value of KDA genes for SS was confirmed using receiver operating 
      characteristic curves. RESULTS: We identified 322 DMRs, annotated as 162 
      associated genes. Six genes were selected via the number of networks of KDA 
      genes. Differential expression of genes such as human leukocyte antigen (HLA) 
      class I, ADAR, and OAS2 was observed in patients' peripheral blood mononuclear 
      cells and the minor salivary glands, which can be used as potential diagnostic 
      biomarkers for SS. CONCLUSION: Clinical sample validation suggested that HLA 
      class I, ADAR, and OAS2 might play a role in the development of SS. Our study 
      shows epigenetic regulatory mechanisms and potential disease markers associated 
      with SS, which in turn will enable us to identify new therapeutic targets.
CI  - (c) 2023 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons 
      Australia, Ltd.
FAU - Lu, Ding-Qi
AU  - Lu DQ
AUID- ORCID: 0000-0003-4503-8486
AD  - Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.
FAU - Yao, Xin-Yi
AU  - Yao XY
AUID- ORCID: 0000-0002-7371-0885
AD  - The Second Clinical Medical College of Zhejiang Chinese Medical University, 
      Hangzhou, People's Republic of China.
FAU - Ren, Ya-Ting
AU  - Ren YT
AD  - The Second Clinical Medical College of Zhejiang Chinese Medical University, 
      Hangzhou, People's Republic of China.
FAU - Zhang, Kai-Yuan
AU  - Zhang KY
AD  - The Second Clinical Medical College of Zhejiang Chinese Medical University, 
      Hangzhou, People's Republic of China.
FAU - Zhu, Xin-Chao
AU  - Zhu XC
AD  - The Second Clinical Medical College of Zhejiang Chinese Medical University, 
      Hangzhou, People's Republic of China.
FAU - Hong, Tao
AU  - Hong T
AD  - The Second Clinical Medical College of Zhejiang Chinese Medical University, 
      Hangzhou, People's Republic of China.
FAU - Yu, Xue
AU  - Yu X
AD  - The Second Clinical Medical College of Zhejiang Chinese Medical University, 
      Hangzhou, People's Republic of China.
FAU - Xie, Zhi-Min
AU  - Xie ZM
AD  - The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 
      People's Republic of China.
FAU - Chen, Li-Ying
AU  - Chen LY
AD  - The Second Clinical Medical College of Zhejiang Chinese Medical University, 
      Hangzhou, People's Republic of China.
FAU - Wang, Xin-Chang
AU  - Wang XC
AUID- ORCID: 0000-0002-4540-301X
AD  - The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 
      People's Republic of China.
LA  - eng
GR  - Y202248715/General Research Project of Education Department of Zhejiang Province/
GR  - 82074341/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230923
PL  - England
TA  - Int J Rheum Dis
JT  - International journal of rheumatic diseases
JID - 101474930
RN  - 0 (Biomarkers)
SB  - IM
MH  - Humans
MH  - *DNA Methylation
MH  - *Sjogren's Syndrome/diagnosis/genetics
MH  - Leukocytes, Mononuclear
MH  - Epigenesis, Genetic
MH  - Biomarkers
OTO - NOTNLM
OT  - DNA methylation
OT  - Sjogren syndrome
OT  - diagnostic markers
OT  - epigenetics
OT  - whole-genome bisulfite sequencing
EDAT- 2023/09/23 11:43
MHDA- 2023/11/02 12:45
CRDT- 2023/09/23 05:27
PHST- 2023/08/08 00:00 [revised]
PHST- 2023/02/07 00:00 [received]
PHST- 2023/09/03 00:00 [accepted]
PHST- 2023/11/02 12:45 [medline]
PHST- 2023/09/23 11:43 [pubmed]
PHST- 2023/09/23 05:27 [entrez]
AID - 10.1111/1756-185X.14918 [doi]
PST - ppublish
SO  - Int J Rheum Dis. 2023 Nov;26(11):2223-2232. doi: 10.1111/1756-185X.14918. Epub 
      2023 Sep 23.