PMID- 37741005
OWN - NLM
STAT- Publisher
LR  - 20231016
IS  - 1873-6750 (Electronic)
IS  - 0160-4120 (Linking)
VI  - 180
DP  - 2023 Oct
TI  - Unveiling the novel role of ryegrass rhizospheric metabolites in benzo[a]pyrene 
      biodegradation.
PG  - 108215
LID - S0160-4120(23)00488-9 [pii]
LID - 10.1016/j.envint.2023.108215 [doi]
AB  - Rhizoremediation is a promising remediation technology for the removal of soil 
      persistent organic pollutants (POPs), especially benzo[a]pyrene (BaP). However, 
      our understanding of the associations among rhizospheric soil metabolites, 
      functional microorganisms, and POPs degradation in different plant growth stages 
      is limited. We combined stable-isotope probing (SIP), high-throughput sequencing, 
      and metabolomics to analyze changes in rhizospheric soil metabolites, functional 
      microbes, and BaP biodegradation in the early growth stages (tillering, jointing) 
      and later stage (booting) of ryegrass. Microbial community structures differed 
      significantly among growth stages. Metabolisms such as benzenoids and carboxylic 
      acids tended to be enriched in the early growth stage, while lipids and organic 
      heterocyclic compounds dominated in the later stage. From SIP, eight 
      BaP-degrading microbes were identified, and most of which such as Ilumatobacter 
      and Singulisphaera were first linked with BaP biodegradation. Notably, the 
      relationship between the differential metabolites and BaP degradation efficiency 
      further suggested that BaP-degrading microbes might metabolize BaP directly to 
      produce benzenoid metabolites (3-hydroxybenzo[a]pyrene), or utilize benzenoids 
      (phyllodulcin) to stimulate the co-metabolism of BaP in early growth stage; some 
      lipids and organic acids, e.g. 1-aminocyclopropane-1-carboxylic acid, might 
      provide nutrients for the degraders to promote BaP metabolism in later stage. 
      Accordingly, we determined that certain rhizospheric metabolites might regulate 
      the rhizospheric microbial communities at different growth stages, and shift the 
      composition and diversity of BaP-degrading bacteria, thereby enhancing in situ 
      BaP degradation. Our study sheds light on POPs rhizoremediation mechanisms in 
      petroleum-contaminated soils.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Zhao, Xuan
AU  - Zhao X
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key 
      Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong 
      Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou 
      Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; 
      College of Architecture and Civil Engineering, Kunming University, Kunming 
      650214, China; CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, 
      China; University of Chinese Academy of Sciences, Beijing 100039, China.
FAU - Li, Jibing
AU  - Li J
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key 
      Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong 
      Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou 
      Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; 
      CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China; 
      University of Chinese Academy of Sciences, Beijing 100039, China. Electronic 
      address: lijibing@gig.ac.cn.
FAU - Zhang, Dayi
AU  - Zhang D
AD  - College of New Energy and Environment, Jilin University, Changchun 130021, China.
FAU - Jiang, Longfei
AU  - Jiang L
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key 
      Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong 
      Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou 
      Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; 
      CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China.
FAU - Wang, Yujie
AU  - Wang Y
AD  - School of Environmental Science and Engineering, Guangdong University of 
      Technology, Guangzhou 510006, China.
FAU - Hu, Beibei
AU  - Hu B
AD  - School of Environmental Science and Engineering, Guangdong University of 
      Technology, Guangzhou 510006, China.
FAU - Wang, Shuang
AU  - Wang S
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key 
      Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong 
      Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou 
      Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; 
      CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China; 
      University of Chinese Academy of Sciences, Beijing 100039, China.
FAU - Dai, Yeliang
AU  - Dai Y
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key 
      Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong 
      Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou 
      Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; 
      CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China; 
      University of Chinese Academy of Sciences, Beijing 100039, China.
FAU - Luo, Chunling
AU  - Luo C
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key 
      Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong 
      Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou 
      Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; 
      CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China. 
      Electronic address: clluo@gig.ac.cn.
FAU - Zhang, Gan
AU  - Zhang G
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key 
      Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong 
      Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou 
      Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; 
      CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China.
LA  - eng
PT  - Journal Article
DEP - 20230916
PL  - Netherlands
TA  - Environ Int
JT  - Environment international
JID - 7807270
SB  - IM
OTO - NOTNLM
OT  - Benzo[a]pyrene-degrading bacteria
OT  - Bioremediation
OT  - Metabolomics
OT  - Persistent organic pollutants degradation
OT  - Plant growth stages
OT  - Stable isotope probing (SIP)
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/24 00:42
MHDA- 2023/09/24 00:42
CRDT- 2023/09/23 18:01
PHST- 2023/05/06 00:00 [received]
PHST- 2023/09/11 00:00 [revised]
PHST- 2023/09/15 00:00 [accepted]
PHST- 2023/09/24 00:42 [pubmed]
PHST- 2023/09/24 00:42 [medline]
PHST- 2023/09/23 18:01 [entrez]
AID - S0160-4120(23)00488-9 [pii]
AID - 10.1016/j.envint.2023.108215 [doi]
PST - ppublish
SO  - Environ Int. 2023 Oct;180:108215. doi: 10.1016/j.envint.2023.108215. Epub 2023 
      Sep 16.