PMID- 37749820
OWN - NLM
STAT- MEDLINE
DCOM- 20240209
LR  - 20240210
IS  - 1470-8744 (Electronic)
IS  - 0885-4513 (Linking)
VI  - 71
IP  - 1
DP  - 2024 Feb
TI  - Tauroursodeoxycholic acid protects Schwann cells from high glucose-induced 
      cytotoxicity by targeting NLRP3 to regulate cell migration and pyroptosis.
PG  - 28-37
LID - 10.1002/bab.2518 [doi]
AB  - Diabetic peripheral neuropathy (DPN) is the most prevalent complication of type 2 
      diabetes mellitus (T2DM), and it seriously affects the quality of life of 
      patients. Tauroursodeoxycholic acid (TUDCA) is a bile acid that plays a 
      protective role against various diseases. However, the function of TUDCA in DPN 
      progression needs to be elucidated. Hence, this study clarified the action of 
      TUDCA on DPN development and explored its mechanism of action. Fecal samples were 
      collected from 50 patients with T2DM or DPN. Schwann cells induced by high levels 
      were constructed to simulate an uncontrolled diabetic state. Cell viability and 
      migration were measured using the CCK-8 and wound-healing assays, respectively. 
      Reactive oxygen species and pyroptosis were detected using flow cytometry. 
      Parabacteroides goldsteinii and Parabacteroides distasonis levels were decreased 
      in the feces of patients with DPN. TUDCA enhanced the viability and migration 
      ability of high glucose-stimulated Schwann cells. In addition, Schwann cell 
      pyroptosis stimulated by high glucose levels was inhibited by TUDCA. Furthermore, 
      the protective roles of TUDCA in cell viability, migration ability, and 
      pyroptosis of Schwann cells stimulated by high glucose were suppressed by the 
      overexpression of NLRP3. TUDCA enhanced cell viability and migration and 
      suppressed pyroptosis in Schwann cells stimulated by high glucose levels by 
      modulating NLRP3 expression. Thus, TUDCA may be a promising drug for DPN therapy.
CI  - (c) 2023 The Authors. Biotechnology and Applied Biochemistry published by Wiley 
      Periodicals LLC on behalf of International Union of Biochemistry and Molecular 
      Biology.
FAU - Wang, Qiuyue
AU  - Wang Q
AD  - Department of Acupuncture and Moxibustion, Shanghai Seventh People's Hospital 
      Affiliated to Shanghai University of Traditional Chinese medicine, Shanghai, 
      China.
FAU - Li, Wen
AU  - Li W
AUID- ORCID: 0000-0002-4801-727X
AD  - Department of Acupuncture and Moxibustion, Shanghai Seventh People's Hospital 
      Affiliated to Shanghai University of Traditional Chinese medicine, Shanghai, 
      China.
FAU - Zhang, Xiaozhuo
AU  - Zhang X
AD  - Department of Acupuncture and Moxibustion, Shanghai Seventh People's Hospital 
      Affiliated to Shanghai University of Traditional Chinese medicine, Shanghai, 
      China.
FAU - Chung, Shuet Ling
AU  - Chung SL
AD  - Department of Acupuncture and Moxibustion, Shanghai Seventh People's Hospital 
      Affiliated to Shanghai University of Traditional Chinese medicine, Shanghai, 
      China.
FAU - Dai, Jinling
AU  - Dai J
AD  - Department of Acupuncture and Moxibustion, Shanghai Seventh People's Hospital 
      Affiliated to Shanghai University of Traditional Chinese medicine, Shanghai, 
      China.
FAU - Jin, Zhu
AU  - Jin Z
AD  - Department of Acupuncture and Moxibustion, Shanghai Seventh People's Hospital 
      Affiliated to Shanghai University of Traditional Chinese medicine, Shanghai, 
      China.
LA  - eng
GR  - 202040002/Shanghai Municipal Commission of Health "A randomized controlled study 
      on the prevention and treatment of metabolic syndrome by Lu's acupuncture and 
      moxibustion for strengthening the spleen and stomach"/
GR  - PWRzj2020-03/The heir to Pudong's traditional Chinese medicine/
GR  - N/A/Shanghai University of Traditional Chinese Medicine Reserve outstanding 
      Chinese medicine talents/
PT  - Journal Article
DEP - 20230925
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 60EUX8MN5X (ursodoxicoltaurine)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - IY9XDZ35W2 (Glucose)
RN  - 516-35-8 (Taurochenodeoxycholic Acid)
SB  - IM
MH  - Humans
MH  - *Pyroptosis
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Glucose/toxicity/metabolism
MH  - *Diabetes Mellitus, Type 2/drug therapy/metabolism
MH  - Quality of Life
MH  - Schwann Cells/metabolism
MH  - Cell Movement
MH  - *Taurochenodeoxycholic Acid
OTO - NOTNLM
OT  - NLRP3
OT  - Schwann cells
OT  - diabetic peripheral neuropathy
OT  - pyroptosis
OT  - tauroursodeoxycholic acid
EDAT- 2023/09/26 06:44
MHDA- 2024/02/09 06:42
CRDT- 2023/09/26 00:33
PHST- 2023/04/26 00:00 [received]
PHST- 2023/09/14 00:00 [accepted]
PHST- 2024/02/09 06:42 [medline]
PHST- 2023/09/26 06:44 [pubmed]
PHST- 2023/09/26 00:33 [entrez]
AID - 10.1002/bab.2518 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2024 Feb;71(1):28-37. doi: 10.1002/bab.2518. Epub 2023 
      Sep 25.