PMID- 37756195
OWN - NLM
STAT- Publisher
LR  - 20230927
IS  - 1477-8920 (Print)
IS  - 1477-8920 (Linking)
VI  - 21
IP  - 9
DP  - 2023 Sep
TI  - Antibiotic resistance and virulence potentials of E. faecalis and E. faecium in 
      hospital wastewater: a case study in Ardabil, Iran.
PG  - 1277-1290
LID - 10.2166/wh.2023.147 [doi]
AB  - Hospital wastewater can contaminate the environment with antibiotic-resistant and 
      virulent bacteria. We analyzed wastewater samples from four hospitals in Ardabil 
      province, Iran for Enterococcus faecium and Enterococcus faecalis using culture 
      and molecular methods. We also performed antimicrobial susceptibility testing and 
      polymerase chain reaction testing for resistance and virulence genes. Out of 141 
      enterococci isolates, 68.8% were E. faecium and 23.4% were E. faecalis. 
      Ciprofloxacin and rifampicin showed the highest level of resistance against E. 
      faecalis and E. faecium isolates at 65%. High-level gentamicin resistance (HLGR), 
      high-level streptomycin resistance (HLSR), ampicillin, and vancomycin resistance 
      were observed in 25, 5, 10, and 5.15% of E. faecium, and 15, 6, 15, and 3.03% of 
      E. faecalis isolates, respectively. The ant(6')-Ia and ant(3')-Ia genes that were 
      responsible for streptomycin resistance were observed in HLSR isolates and 
      aph(3')-IIIa and aac(6') Ie-aph(2'')-Ia genes accounting for gentamicin resistance 
      were detected in HLGR isolates. vanA was the predominant gene detected in 
      vancomycin-resistant isolates. The majority of isolates were positive for gelE, 
      asa1, esp, cylA, and hyl virulence genes. We found that drug-resistant and 
      virulent E. faecalis and E. faecium isolates were prevalent in hospital 
      wastewater. Proper treatment strategies are required to prevent their 
      dissemination into the environment.
FAU - Jannati, Elham
AU  - Jannati E
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran; Department of Microbiology, School of Sciences, Islamic Azad 
      University, Ardabil Branch, Ardabil, Iran E-mail: m.arzanlou@arums.ac.ir; 
      arzanlo53@gmail.com.
FAU - Khademi, Farzad
AU  - Khademi F
AD  - Department of Microbiology, School of Medicine, Ardabil University of Medical 
      Sciences, Ardabil, Iran.
FAU - Manouchehrifar, Meysam
AU  - Manouchehrifar M
AD  - Food Control Laboratory, Ardabil University of Medical Sciences, Ardabil, Iran.
FAU - Maleki, Dadras
AU  - Maleki D
AD  - Microbiology Laboratory, Imam Hospital, Ardabil University of Medical Sciences, 
      Ardabil, Iran.
FAU - Amirmozaffari, Nour
AU  - Amirmozaffari N
AD  - Department of Microbiology, School of Medicine, Iran University of Medical 
      Sciences, Tehran, Iran.
FAU - Sadat Nikbin, Vajihe
AU  - Sadat Nikbin V
AD  - Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
FAU - Arzanlou, Mohsen
AU  - Arzanlou M
AUID- ORCID: 0000-0002-4233-9497
AD  - Department of Microbiology, School of Medicine, Ardabil University of Medical 
      Sciences, Ardabil, Iran.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Water Health
JT  - Journal of water and health
JID - 101185420
SB  - IM
EDAT- 2023/09/27 18:42
MHDA- 2023/09/27 18:42
CRDT- 2023/09/27 12:44
PHST- 2023/09/27 18:42 [medline]
PHST- 2023/09/27 18:42 [pubmed]
PHST- 2023/09/27 12:44 [entrez]
AID - 10.2166/wh.2023.147 [doi]
PST - ppublish
SO  - J Water Health. 2023 Sep;21(9):1277-1290. doi: 10.2166/wh.2023.147.