PMID- 37759147
OWN - NLM
STAT- MEDLINE
DCOM- 20240104
LR  - 20240104
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 37
IP  - 1
DP  - 2024 Jan
TI  - Isolation of tumor stem-like cells from primary laryngeal squamous cell carcinoma 
      cells (FD-LS-6).
PG  - 323-336
LID - 10.1007/s13577-023-00984-6 [doi]
AB  - The development of efficient treatments for laryngeal squamous cell carcinoma 
      (LSCC) is hindered by the lack of applicable tumor cell lines and animal models 
      of the disease, especially those related to cancer stem-like cells (CSCs). CSCs 
      play critical roles in tumor propagation and pathogenesis whereas no CSCs lines 
      have been developed to date. In this study, we establish an LSCC cell line 
      (FD-LS-6) from primary LSCC tumor tissue (not experienced single-cell cloning) 
      and adapted a culturing condition for the expansion of potential stem cells 
      (EPSCs) to isolate CSCs from FD-LS-6. We successfully derived novel CSCs and 
      named them as LSCC sphere-forming cells (LSCSCs) which were subsequently 
      characterized for their CSC properties. We showed that LSCSCs shared many 
      properties of CSCs, including CSC marker, robust self-renewal capacity, 
      tumorigenesis ability, potential to generate other cell types such as adipocytes 
      and osteoblasts, and resistance to chemotherapy. Compared to parental cells, 
      LSCSCs were significantly more potent in forming tumors in vivo in mice and more 
      resistant to chemotherapy. LSCSCs have higher expressions of 
      epithelial-mesenchymal transition proteins and chemotherapy resistance factors, 
      and exhibit an activated COX2/PEG2 signaling pathway. Altogether, our work 
      establishes the first CSCs of LSCC (FD-LS-6) and provides a tool to study 
      tumorigenesis and metastasis of LSCC and help the development of anticancer 
      therapies.
CI  - (c) 2023. The Author(s) under exclusive licence to Japan Human Cell Society.
FAU - Zhang, Duo
AU  - Zhang D
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai, 200031, China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei 
      Road, Shanghai, 201300, China.
FAU - Tang, Di
AU  - Tang D
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai, 200031, China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei 
      Road, Shanghai, 201300, China.
FAU - Liu, Pen-Tao
AU  - Liu PT
AD  - School of Biomedical Sciences, Stem Cell and Regenerative Medicine Consortium, Li 
      Ka Shing Faculty of Medicine, The University of Hong Kong, 5 Sassoon Road, Hong 
      Kong, China.
AD  - Centre for Translational Stem Cell Biology, Science and Technology Park, 6-8 
      Harbour Road, Hong Kong, China.
FAU - Tao, Lei
AU  - Tao L
AUID- ORCID: 0000-0003-3384-9396
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, 83 Fenyang Road, Shanghai, 200031, China. doctortaolei@163.com.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei 
      Road, Shanghai, 201300, China. doctortaolei@163.com.
FAU - Lu, Li-Ming
AU  - Lu LM
AD  - Shanghai Institute of Immunology, Shanghai Jiao Tong University School of 
      Medicine, 280 South Chongqing Road, Shanghai, 200025, China. lulunew2003@163.com.
LA  - eng
GR  - 82103316/National Natural Science Foundation of China/
GR  - 82203506/National Natural Science Foundation of China/
GR  - 30801283/National Natural Science Foundation of China/
GR  - 30972691/National Natural Science Foundation of China/
GR  - 82071856，81671579/National Natural Science Foundation of China/
GR  - 31370904/National Natural Science Foundation of China/
GR  - 22490760400/Program for scientific and technological innovation from the Science 
      and Technology Commission of Shanghai Municipality/
GR  - 2020JP009/Shanghai Municipal Commission of Health, Scientific Research Program of 
      Traditional Chinese medicine/
GR  - 16SG14/Shuguang Planning of Shanghai Municipal Education Commission/
GR  - 2020YFA0113101/The National Key Research and Development Program/
PT  - Journal Article
DEP - 20230927
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
SB  - IM
MH  - Mice
MH  - Animals
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Cell Line, Tumor
MH  - *Epithelial-Mesenchymal Transition
MH  - *Head and Neck Neoplasms/pathology
MH  - Carcinogenesis/pathology
MH  - Neoplastic Stem Cells/pathology
MH  - Gene Expression Regulation, Neoplastic
MH  - Cell Proliferation
OTO - NOTNLM
OT  - CSCs; Epithelial-mesenchymal transition
OT  - EMT
OT  - LSCC; LSCC sphere-forming cells
OT  - LSCSCs; Cancer stem-like cells
OT  - Laryngeal squamous cell carcinoma
EDAT- 2023/09/28 00:42
MHDA- 2024/01/04 11:44
CRDT- 2023/09/27 23:40
PHST- 2023/03/24 00:00 [received]
PHST- 2023/09/09 00:00 [accepted]
PHST- 2024/01/04 11:44 [medline]
PHST- 2023/09/28 00:42 [pubmed]
PHST- 2023/09/27 23:40 [entrez]
AID - 10.1007/s13577-023-00984-6 [pii]
AID - 10.1007/s13577-023-00984-6 [doi]
PST - ppublish
SO  - Hum Cell. 2024 Jan;37(1):323-336. doi: 10.1007/s13577-023-00984-6. Epub 2023 Sep 
      27.