PMID- 37759297
OWN - NLM
STAT- MEDLINE
DCOM- 20230929
LR  - 20231121
IS  - 1477-3155 (Electronic)
IS  - 1477-3155 (Linking)
VI  - 21
IP  - 1
DP  - 2023 Sep 28
TI  - Natural exosomes-like nanoparticles in mung bean sprouts possesses anti-diabetic 
      effects via activation of PI3K/Akt/GLUT4/GSK-3beta signaling pathway.
PG  - 349
LID - 10.1186/s12951-023-02120-w [doi]
LID - 349
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease 
      characterized by hyperglycemia and insulin resistance. Mung bean sprouts are 
      traditionally considered a "folk" hypoglycemic food and their pharmacological 
      effects and underlying mechanisms warrant further investigation. PURPOSE: This 
      study aimed to investigate the anti-diabetic effects of the exosomes-like 
      nanoparticles in mung bean sprouts (MELNs) and explore the related molecular 
      mechanisms. RESULTS: MELNs were isolated using a differential 
      centrifugation-polyethylene glycol (PEG) method, and the identification of MELNs 
      were confirmed by PAGE gel electrophoresis, agarose gel electrophoresis, 
      thin-layer chromatography (TLC), and transmission electron microscopy (TEM). In 
      the high-fat diet/streptozotocin (HFD/STZ) mouse model, MELNs ameliorated the 
      progression of T2DM by increasing oral glucose tolerance test (OGTT) and insulin 
      tolerance test (ITT) results, decreasing the fasting blood glucose level, and 
      reducing the serum triglycerides (TG) and total cholesterol (TC). 
      Histopathological examinations indicated MELNs diminished inflammatory 
      infiltration of hepatocytes and amplified the area of islet B cells. In addition, 
      MELNs decreased the oxidative stress levels in liver tissue and had good 
      biocompatibility. In vitro experiments verified that MELNs improved the viability 
      of glucosamine (GlcN) induced insulin-resistant hepatocytes. Furthermore, this 
      study also revealed that MELNs upregulated GLUT4 & Nrf2 and down-regulated GSK-3beta 
      via activating the PI3K/Akt signaling pathway, promoting the production of 
      antioxidant enzymes, such as HO-1 and SOD, to reduce oxidative stress. 
      CONCLUSION: MELNs mitigated the progression of type 2 diabetes in HFD/STZ mouse 
      model. The underlying molecular mechanism is related to PI3K/Akt/GLUT4/GSK-3beta 
      signaling pathway.
CI  - (c) 2023. BioMed Central Ltd., part of Springer Nature.
FAU - He, Chengxun
AU  - He C
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China.
FAU - Wang, Ke
AU  - Wang K
AD  - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, 
      China.
FAU - Xia, Jun
AU  - Xia J
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China.
FAU - Qian, Die
AU  - Qian D
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China.
FAU - Guo, Juan
AU  - Guo J
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China.
FAU - Zhong, Lian
AU  - Zhong L
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China.
FAU - Tang, Dandan
AU  - Tang D
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China.
FAU - Chen, Xiuping
AU  - Chen X
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Peng, Wei
AU  - Peng W
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China. pengwei@cdutcm.edu.cn.
FAU - Chen, Yunhui
AU  - Chen Y
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China. chenyunhui@cdutcm.edu.cn.
FAU - Tang, Yong
AU  - Tang Y
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, School of Basic Medical Sciences, School of Health and Rehabilitation, 
      CDUTCM-KEELE Joint Health and Medical Sciences Institute, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, 611137, China. tangyong@cdutcm.edu.cn.
LA  - eng
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
GR  - 2021MS464/Sichuan Provincial Administration of Traditional Chinese Medicine/
PT  - Journal Article
DEP - 20230928
PL  - England
TA  - J Nanobiotechnology
JT  - Journal of nanobiotechnology
JID - 101152208
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - 0 (Insulin)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - Glycogen Synthase Kinase 3 beta
MH  - Phosphatidylinositol 3-Kinases
MH  - Proto-Oncogene Proteins c-akt
MH  - *Vigna
MH  - *Exosomes
MH  - Insulin
MH  - *Nanoparticles
MH  - Disease Models, Animal
MH  - Signal Transduction
PMC - PMC10536756
OTO - NOTNLM
OT  - Exosome-like nanoparticles
OT  - Mung bean sprouts
OT  - Oxidative stress
OT  - Type 2 diabetes Mellitus
COIS- The authors declare no competing interests.
EDAT- 2023/09/28 00:42
MHDA- 2023/09/29 06:44
PMCR- 2023/09/28
CRDT- 2023/09/27 23:51
PHST- 2023/07/18 00:00 [received]
PHST- 2023/09/20 00:00 [accepted]
PHST- 2023/09/29 06:44 [medline]
PHST- 2023/09/28 00:42 [pubmed]
PHST- 2023/09/27 23:51 [entrez]
PHST- 2023/09/28 00:00 [pmc-release]
AID - 10.1186/s12951-023-02120-w [pii]
AID - 2120 [pii]
AID - 10.1186/s12951-023-02120-w [doi]
PST - epublish
SO  - J Nanobiotechnology. 2023 Sep 28;21(1):349. doi: 10.1186/s12951-023-02120-w.