PMID- 37759996
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231003
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 12
IP  - 9
DP  - 2023 Aug 30
TI  - Antioxidant and Anti-Inflammatory Activities of High-Glucosinolate-Synthesis 
      Lines of Brassica rapa.
LID - 10.3390/antiox12091693 [doi]
LID - 1693
AB  - Excessive oxidative stress and inflammatory responses are associated with the 
      development of various diseases, including cancer. Glucosinolates (GSLs) are 
      phytochemicals known for their antioxidant properties, and doubled haploid lines 
      (DHLs) of Brassica rapa with high GSL contents (HGSL) were intentionally 
      developed from two edible subspecies of Brassica rapa: B. rapa subsp. 
      trilocularis and B. rapa subsp. chinensis. The purpose of the present study is to 
      assess the capacity of HGSL DHLs to mitigate oxidative stress and inflammation in 
      lipopolysaccharide (LPS)-stimulated RAW264.7 cells, compared to pak choi as a 
      parental control. Our findings demonstrate that HGSL DH lines effectively 
      suppressed the expression of inducible nitric oxide synthase, leading to the 
      reduced levels of nitric oxide at non-toxic concentrations. Additionally, these 
      lines exhibited scavenging activity against reactive oxygen species and free 
      radicals. The enhanced antioxidant capacity of HGSL DHLs was mechanistically 
      attributed to the upregulation of antioxidant enzymes, such as NADPH quinone 
      oxidoreductase 1 (NQO1), the glutamate-cysteine ligase catalytic subunit (GCLC), 
      and heme oxygenase-1 (HMOX1). Furthermore, we confirmed that these effects were 
      mediated through the nuclear factor erythroid 2-related factor 2 (NRF2) signaling 
      pathway via p38 phosphorylation. Moreover, HGSL DHLs demonstrated inhibitory 
      effects on pro-inflammatory cytokines and signal transducers and activators of 
      transcription 3 (STAT3) phosphorylation. Collectively, our results indicate that 
      HGSL DHLs possess better antioxidant and anti-inflammatory properties compared to 
      the parental control pak choi in LPS-stimulated RAW264.7 cells, suggesting that 
      HGSL DHLs of Brassica rapa could be considered as a beneficial daily vegetable 
      for reducing the risk of inflammation-associated diseases.
FAU - Choi, Hyunjin
AU  - Choi H
AD  - Department of Biomedical Science and Technology, Kyung Hee University, Seoul 
      02447, Republic of Korea.
AD  - Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
FAU - Kim, Hail
AU  - Kim H
AD  - Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
FAU - Han, Sanghee
AU  - Han S
AUID- ORCID: 0000-0002-4445-5168
AD  - Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
FAU - Park, Hyun Woo
AU  - Park HW
AD  - Genomic Division, Department of Agricultural Bio-Resources, National Institute of 
      Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic 
      of Korea.
FAU - Ha, In Jin
AU  - Ha IJ
AUID- ORCID: 0000-0002-9210-1401
AD  - Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
FAU - Kim, Jung Sun
AU  - Kim JS
AUID- ORCID: 0000-0001-5209-4481
AD  - Genomic Division, Department of Agricultural Bio-Resources, National Institute of 
      Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic 
      of Korea.
FAU - Lee, Seok-Geun
AU  - Lee SG
AUID- ORCID: 0000-0002-0060-5777
AD  - Department of Biomedical Science and Technology, Kyung Hee University, Seoul 
      02447, Republic of Korea.
AD  - Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
LA  - eng
GR  - PJ015780022023/Rural Development Administration/
GR  - NRF-2021R1I1A2054560/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20230830
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC10525794
OTO - NOTNLM
OT  - Brassica rapa
OT  - NRF2
OT  - antioxidant
OT  - doubled haploid lines
OT  - glucosinolate
OT  - inflammation
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/28 06:42
MHDA- 2023/09/28 06:43
PMCR- 2023/08/30
CRDT- 2023/09/28 01:04
PHST- 2023/07/21 00:00 [received]
PHST- 2023/08/25 00:00 [revised]
PHST- 2023/08/29 00:00 [accepted]
PHST- 2023/09/28 06:43 [medline]
PHST- 2023/09/28 06:42 [pubmed]
PHST- 2023/09/28 01:04 [entrez]
PHST- 2023/08/30 00:00 [pmc-release]
AID - antiox12091693 [pii]
AID - antioxidants-12-01693 [pii]
AID - 10.3390/antiox12091693 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2023 Aug 30;12(9):1693. doi: 10.3390/antiox12091693.