PMID- 37762125
OWN - NLM
STAT- Publisher
LR  - 20230930
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 18
DP  - 2023 Sep 7
TI  - Exploring the Effects of Human Bone Marrow-Derived Mononuclear Cells on 
      Angiogenesis In Vitro.
LID - 10.3390/ijms241813822 [doi]
LID - 13822
AB  - Cell therapies involving the administration of bone marrow-derived mononuclear 
      cells (BM-MNCs) for patients with chronic limb-threatening ischemia (CLTI) have 
      shown promise; however, their overall effectiveness lacks evidence, and the exact 
      mechanism of action remains unclear. In this study, we examined the angiogenic 
      effects of well-controlled human bone marrow cell isolates on endothelial cells. 
      The responses of endothelial cell proliferation, migration, tube formation, and 
      aortic ring sprouting were analyzed in vitro, considering both the direct and 
      paracrine effects of BM cell isolates. Furthermore, we conducted these 
      investigations under both normoxic and hypoxic conditions to simulate the 
      ischemic environment. Interestingly, no significant effect on the angiogenic 
      response of human umbilical vein endothelial cells (HUVECs) following treatment 
      with BM-MNCs was observed. This study fails to provide significant evidence for 
      angiogenic effects from human bone marrow cell isolates on human endothelial 
      cells. These in vitro experiments suggest that the potential benefits of BM-MNC 
      therapy for CLTI patients may not involve endothelial cell angiogenesis.
FAU - Peeters, Judith A H M
AU  - Peeters JAHM
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands.
AD  - Einthoven Laboratory for Experimental Vascular Medicine, Leiden University 
      Medical Center, 2300 RC Leiden, The Netherlands.
FAU - Peters, Hendrika A B
AU  - Peters HAB
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands.
AD  - Einthoven Laboratory for Experimental Vascular Medicine, Leiden University 
      Medical Center, 2300 RC Leiden, The Netherlands.
FAU - Videler, Anique J
AU  - Videler AJ
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands.
AD  - Einthoven Laboratory for Experimental Vascular Medicine, Leiden University 
      Medical Center, 2300 RC Leiden, The Netherlands.
FAU - Hamming, Jaap F
AU  - Hamming JF
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands.
FAU - Schepers, Abbey
AU  - Schepers A
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands.
FAU - Quax, Paul H A
AU  - Quax PHA
AUID- ORCID: 0000-0002-6853-5760
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands.
AD  - Einthoven Laboratory for Experimental Vascular Medicine, Leiden University 
      Medical Center, 2300 RC Leiden, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20230907
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
PMC - PMC10531254
OTO - NOTNLM
OT  - angiogenesis
OT  - arteriogenesis
OT  - bone marrow-derived mononuclear cells
OT  - cell therapy
OT  - chronic limb-threatening ischemia
OT  - peripheral artery disease
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/28 06:42
MHDA- 2023/09/28 06:42
PMCR- 2023/09/07
CRDT- 2023/09/28 01:17
PHST- 2023/08/09 00:00 [received]
PHST- 2023/08/29 00:00 [revised]
PHST- 2023/09/01 00:00 [accepted]
PHST- 2023/09/28 06:42 [medline]
PHST- 2023/09/28 06:42 [pubmed]
PHST- 2023/09/28 01:17 [entrez]
PHST- 2023/09/07 00:00 [pmc-release]
AID - ijms241813822 [pii]
AID - ijms-24-13822 [pii]
AID - 10.3390/ijms241813822 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Sep 7;24(18):13822. doi: 10.3390/ijms241813822.