PMID- 37762201
OWN - NLM
STAT- Publisher
LR  - 20231003
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 18
DP  - 2023 Sep 9
TI  - Regulation of Embden-Meyerhof-Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) 
      Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (Carica 
      papaya L.).
LID - 10.3390/ijms241813898 [doi]
LID - 13898
AB  - Postharvest abnormal chilling injury (CI) behavior in papaya (Carica papaya L.) 
      fruit is a rare phenomenon that may be associated with respiratory metabolism. 
      This study thus aimed to investigate the impacts of storage temperatures (1 and 6 
       degrees C) on the respiratory metabolism of postharvest papaya and its impact on CI 
      development. Results demonstrated that 1  degrees C storage reduced the activities of 
      hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), citrate 
      synthase (CS), and alpha-ketoglutarate dehydrogenase (alpha-KGDH) and regulated the 
      expression of corresponding enzymes in the Embden-Meyerhof-Parnas (EMP) pathway 
      and tricarboxylic acid (TCA) cycle compared with 6  degrees C storage, resulting in a 
      lower respiration rate of the EMP-TCA pathway and mitigating the development of 
      CI. Meanwhile, lower contents of nicotinamide adenine dinucleotide (hydrogen) 
      (NAD(H)) were observed in papaya fruit stored at 1  degrees C. Notably, papaya fruit 
      stored at 1  degrees C maintained higher activity and transcriptional levels of SDH and 
      IDH during the whole storage period. These findings suggest that 1  degrees C storage 
      reduced the respiration rate of the EMP-TCA pathway by reducing the expression 
      level and activity of related enzymes, which is conducive to the reduction of 
      respiration substrate consumption and finally alleviating the occurrence of CI.
FAU - Huang, Lijin
AU  - Huang L
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
FAU - Tao, Shoukui
AU  - Tao S
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
FAU - Zhu, Yi
AU  - Zhu Y
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
FAU - Pan, Yonggui
AU  - Pan Y
AUID- ORCID: 0000-0002-2866-2298
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
AD  - Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Haikou 
      570228, China.
FAU - Zhang, Zhengke
AU  - Zhang Z
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
FAU - Yu, Zhiqian
AU  - Yu Z
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
FAU - Chen, Yezhen
AU  - Chen Y
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
LA  - eng
GR  - 31860577; 32160586/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230909
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
PMC - PMC10530671
OTO - NOTNLM
OT  - EMP pathway
OT  - TCA cycle
OT  - chilling injury
OT  - papaya
OT  - respiration pathway
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/28 06:43
MHDA- 2023/09/28 06:43
PMCR- 2023/09/09
CRDT- 2023/09/28 01:17
PHST- 2023/07/26 00:00 [received]
PHST- 2023/08/24 00:00 [revised]
PHST- 2023/09/02 00:00 [accepted]
PHST- 2023/09/28 06:43 [medline]
PHST- 2023/09/28 06:43 [pubmed]
PHST- 2023/09/28 01:17 [entrez]
PHST- 2023/09/09 00:00 [pmc-release]
AID - ijms241813898 [pii]
AID - ijms-24-13898 [pii]
AID - 10.3390/ijms241813898 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Sep 9;24(18):13898. doi: 10.3390/ijms241813898.