PMID- 37762609
OWN - NLM
STAT- MEDLINE
DCOM- 20230929
LR  - 20231003
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 18
DP  - 2023 Sep 19
TI  - Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways 
      in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex 
      Differentiation.
LID - 10.3390/ijms241814306 [doi]
LID - 14306
AB  - This study investigated the potential to use double-stranded RNA insulin-like 
      androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium 
      nipponense and identified the molecular mechanisms underlying crustacean 
      reproduction and sex differentiation. The study aimed to determine whether dsIAG 
      could induce sex reversal in PL30-male M. nipponense during a critical period. 
      The sex-related genes were selected by performing the gonadal transcriptome 
      analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed 
      individuals (dsRM), and unreversed males (dsNRM). After six injections, the 
      experiment finally resulted in a 20% production of dsRM. Histologically, dsRM 
      ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A 
      total of 1718, 1069, and 255 differentially expressed genes were identified 
      through transcriptome sequencing of the gonads in three comparison groups, 
      revealing crucial genes related to reproduction and sex differentiation, such as 
      GnRHR, VGR, SG, and LWS. Principal Component Analysis (PCA) also distinguished 
      dsM and dsRM very well. In addition, this study predicted that the eyestalks and 
      the "phototransduction-fly" photoperiodic pathways of M. nipponense could play an 
      important role in sex reversal. The enrichment of related pathways and growth 
      traits in dsNRM were combined to establish that IAG played a significant role in 
      reproduction, growth regulation, and metabolism. Finally, complete sex reversal 
      may depend on specific stimuli at critical periods. Overall, this study provides 
      valuable findings for the IAG regulation of sex differentiation, reproduction, 
      and growth of M. nipponense in establishing a monoculture.
FAU - Cai, Pengfei
AU  - Cai P
AUID- ORCID: 0000-0001-5091-7755
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Zhang, Wenyi
AU  - Zhang W
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
FAU - Jiang, Sufei
AU  - Jiang S
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
FAU - Xiong, Yiwei
AU  - Xiong Y
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
FAU - Yuan, Huwei
AU  - Yuan H
AUID- ORCID: 0000-0002-5551-4960
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Gao, Zijian
AU  - Gao Z
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Gao, Xuanbing
AU  - Gao X
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Ma, Cheng
AU  - Ma C
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Zhou, Yongkang
AU  - Zhou Y
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Gong, Yongsheng
AU  - Gong Y
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
FAU - Qiao, Hui
AU  - Qiao H
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
FAU - Jin, Shubo
AU  - Jin S
AUID- ORCID: 0000-0002-6550-9268
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
FAU - Fu, Hongtuo
AU  - Fu H
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
LA  - eng
GR  - BK20221207/the Natural Science Foundation of Jiangsu Province/
GR  - 2020TD36/Central Public-Interest Scientific Institution Basal Research Fund CAFS/
GR  - JBGS (2021) 118/Seed Industry Revitalization Project of Jiangsu Province/
GR  - PZCZ201745/Jiangsu Agricultural Industry Technology System, earmarked fund for 
      CARS-48, and New Cultivar Breeding Major Project of Jiangsu Province/
PT  - Journal Article
DEP - 20230919
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Insulin)
RN  - 0 (Androgens)
RN  - 0 (Insulin, Regular, Human)
SB  - IM
MH  - Humans
MH  - Female
MH  - Male
MH  - Animals
MH  - *Insulin
MH  - Androgens/pharmacology
MH  - *Palaemonidae/genetics
MH  - Sex Differentiation/genetics
MH  - Insulin, Regular, Human
MH  - Reproduction/genetics
PMC - PMC10531965
OTO - NOTNLM
OT  - IAG
OT  - Macrobrachium nipponense
OT  - growth regulation
OT  - ovary
OT  - photoperiodic pathways
OT  - reproduction
OT  - sex reversal
OT  - testis
OT  - transcriptome
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/28 06:43
MHDA- 2023/09/29 06:43
PMCR- 2023/09/19
CRDT- 2023/09/28 01:21
PHST- 2023/07/24 00:00 [received]
PHST- 2023/09/13 00:00 [revised]
PHST- 2023/09/18 00:00 [accepted]
PHST- 2023/09/29 06:43 [medline]
PHST- 2023/09/28 06:43 [pubmed]
PHST- 2023/09/28 01:21 [entrez]
PHST- 2023/09/19 00:00 [pmc-release]
AID - ijms241814306 [pii]
AID - ijms-24-14306 [pii]
AID - 10.3390/ijms241814306 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Sep 19;24(18):14306. doi: 10.3390/ijms241814306.