PMID- 37763977
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231003
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 11
IP  - 9
DP  - 2023 Aug 23
TI  - Novel and Simple Method for Quantification of 2,4,6-Trichlorophenol with 
      Microbial Conversion to 2,4,6-Trichloroanisole.
LID - 10.3390/microorganisms11092133 [doi]
LID - 2133
AB  - Contamination with 2,4,6-trichloroanisole (TCA) often causes taste and odor (T&O) 
      problems in drinking water due to its low odor threshold concentration. Microbial 
      O-methylation of the precursor 2,4,6-trichlorophenol (TCP) would be the dominant 
      mechanism for TCA formation. Simple and rapid measurement of TCP in the low 
      concentration range is necessary to control the problems induced by TCA. In this 
      study, the combination of microbial conversion and instrumental analysis was 
      proposed as a method of TCP quantification. Fungi and bacteria were isolated from 
      various water samples and examined for their ability to produce TCA from TCP. As 
      a result, a strain exhibiting quantitative TCA production and a high growth rate 
      was obtained and named Mycolicibacterium sp. CB14. The conversion rate of TCP to 
      TCA by this strain was found to be high and stable (85.9 +/- 5.3%), regardless of 
      the applied TCP concentration, although within the range of 0.1-10 microg/L. The 
      limits of detection and quantification for TCP by this proposed method were 
      determined to be 5.2 ng/L and 17.3 ng/L, respectively. By improving the methods, 
      Mycolicibacterium sp. CB14 could be used for the quantification of TCP at very 
      low concentration levels, which is sufficient to manage the T&O problem caused by 
      TCA.
FAU - Goto, Saki
AU  - Goto S
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo 
      192-0982, Japan.
FAU - Urase, Taro
AU  - Urase T
AUID- ORCID: 0000-0002-5173-8334
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo 
      192-0982, Japan.
FAU - Nakakura, Kaito
AU  - Nakakura K
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo 
      192-0982, Japan.
LA  - eng
GR  - 20K04758/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20230823
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10535749
OTO - NOTNLM
OT  - 2,4,6-trichloroanisole (TCA)
OT  - 2,4,6-trichlorophenol (TCP)
OT  - O-methylation
OT  - odor threshold
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2023/09/28 06:42
MHDA- 2023/09/28 06:43
PMCR- 2023/08/23
CRDT- 2023/09/28 01:29
PHST- 2023/07/15 00:00 [received]
PHST- 2023/08/14 00:00 [revised]
PHST- 2023/08/21 00:00 [accepted]
PHST- 2023/09/28 06:43 [medline]
PHST- 2023/09/28 06:42 [pubmed]
PHST- 2023/09/28 01:29 [entrez]
PHST- 2023/08/23 00:00 [pmc-release]
AID - microorganisms11092133 [pii]
AID - microorganisms-11-02133 [pii]
AID - 10.3390/microorganisms11092133 [doi]
PST - epublish
SO  - Microorganisms. 2023 Aug 23;11(9):2133. doi: 10.3390/microorganisms11092133.