PMID- 37764432
OWN - NLM
STAT- MEDLINE
DCOM- 20230929
LR  - 20240210
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 28
IP  - 18
DP  - 2023 Sep 16
TI  - Immunomodulatory Activity of Polysaccharides Isolated from Saussurea salicifolia 
      L. and Saussurea frolovii Ledeb.
LID - 10.3390/molecules28186655 [doi]
LID - 6655
AB  - The genus Saussurea has been used in the preparation of therapies for a number of 
      medical problems, yet not much is known about the therapeutic 
      high-molecular-weight compounds present in extracts from these plants. Since 
      polysaccharides are important in immune modulation, we investigated the chemical 
      composition and immunomodulatory activity of Saussurea salicifolia L. and 
      Saussurea frolovii Ledeb polysaccharides. Water-soluble polysaccharides from the 
      aerial parts of these plants were extracted using water at pHs of 2 and 6 and 
      subsequently precipitated in ethanol to obtain fractions SSP2 and SSP6 from S. 
      salicifolia and fractions SSF2 and SSF6 from S. frolovii. The molecular weights 
      of fractions SSP2, SSP6, SFP2, and SFP6 were estimated to be 143.7, 113.2, 75.3, 
      and 64.3 kDa, respectively. The polysaccharides from S. frolovii contained xylose 
      (67.1-71.7%) and glucose (28.3-32.9%), whereas the polysaccharides from S. 
      frolovii contained xylose (63.1-76.7%), glucose (11.8-19.2%), galactose 
      (4.7-8.3%), and rhamnose (6.8-9.4%). Fractions SSP2, SSP6, and SFP2 stimulated 
      nitric oxide (NO) production by murine macrophages, and NO production induced by 
      SSP2, SSP6, and SFP2 was not inhibited by polymyxin B treatment of the fractions, 
      whereaspolymyxin B treatment diminished the effects of SFP6, suggesting that SFP6 
      could contain lipopolysaccharide (LPS). The LPS-free fractions SSP2, SSP6, and 
      SFP2 had potent immunomodulatory activity, induced NO production, and activated 
      transcription factors NF-kappaB/AP-1 in human monocytic THP-1 cells and cytokine 
      production by human MonoMac-6 monocytic cells, including interleukin (IL)-1alpha, 
      IL-1beta, IL-6, granulocyte macrophage colony-stimulating factor (GM-CSF), 
      interferon-gamma, monocyte chemotactic protein 1 (MCP-1), and tumor necrosis factor 
      (TNF). These data suggest that at least part of the beneficial therapeutic 
      effects reported for water extracts of the Saussurea species are due to the 
      modulation of leukocyte functions by polysaccharides.
FAU - Schepetkin, Igor A
AU  - Schepetkin IA
AD  - Department of Microbiology and Cell Biology, Montana State University, Bozeman, 
      MT 59717, USA.
FAU - Danilets, Marina G
AU  - Danilets MG
AD  - Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk 
      NRMC, Tomsk 634050, Russia.
FAU - Ligacheva, Anastasia A
AU  - Ligacheva AA
AD  - Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk 
      NRMC, Tomsk 634050, Russia.
FAU - Trofimova, Evgenia S
AU  - Trofimova ES
AD  - Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk 
      NRMC, Tomsk 634050, Russia.
AD  - Pharmaceutical Faculty, Siberian State Medical University, Tomsk 634050, Russia.
FAU - Selivanova, Natalia S
AU  - Selivanova NS
AD  - Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk 
      NRMC, Tomsk 634050, Russia.
FAU - Sherstoboev, Evgenii Yu
AU  - Sherstoboev EY
AUID- ORCID: 0000-0002-6178-5329
AD  - Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk 
      NRMC, Tomsk 634050, Russia.
FAU - Krivoshchekov, Sergei V
AU  - Krivoshchekov SV
AUID- ORCID: 0000-0001-5505-7141
AD  - Pharmaceutical Faculty, Siberian State Medical University, Tomsk 634050, Russia.
FAU - Gulina, Ekaterina I
AU  - Gulina EI
AUID- ORCID: 0000-0002-3234-6845
AD  - Pharmaceutical Faculty, Siberian State Medical University, Tomsk 634050, Russia.
FAU - Brazovskii, Konstantin S
AU  - Brazovskii KS
AD  - Pharmaceutical Faculty, Siberian State Medical University, Tomsk 634050, Russia.
AD  - Research School of Chemistry and Applied Biomedical Sciences, National Research 
      Tomsk Polytechnic University, Tomsk 634050, Russia.
FAU - Kirpotina, Liliya N
AU  - Kirpotina LN
AD  - Department of Microbiology and Cell Biology, Montana State University, Bozeman, 
      MT 59717, USA.
FAU - Quinn, Mark T
AU  - Quinn MT
AUID- ORCID: 0000-0001-8114-5073
AD  - Department of Microbiology and Cell Biology, Montana State University, Bozeman, 
      MT 59717, USA.
FAU - Belousov, Mikhail V
AU  - Belousov MV
AUID- ORCID: 0000-0002-2153-7945
AD  - Pharmaceutical Faculty, Siberian State Medical University, Tomsk 634050, Russia.
LA  - eng
GR  - P20 GM103474/GM/NIGMS NIH HHS/United States
GR  - U54 GM115371/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20230916
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - A1TA934AKO (Xylose)
RN  - 0 (Polysaccharides)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 0 (Lipopolysaccharides)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Mice
MH  - *Saussurea
MH  - Xylose
MH  - Polysaccharides/pharmacology
MH  - Interferon-gamma
MH  - Lipopolysaccharides/pharmacology
MH  - Glucose
PMC - PMC10536955
OTO - NOTNLM
OT  - Saussurea
OT  - cytokine
OT  - macrophage
OT  - nitric oxide
OT  - plant polysaccharide
OT  - polymyxin B
OT  - xyloglucan
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/28 06:42
MHDA- 2023/09/29 06:44
PMCR- 2023/09/16
CRDT- 2023/09/28 01:32
PHST- 2023/07/28 00:00 [received]
PHST- 2023/09/11 00:00 [revised]
PHST- 2023/09/13 00:00 [accepted]
PHST- 2023/09/29 06:44 [medline]
PHST- 2023/09/28 06:42 [pubmed]
PHST- 2023/09/28 01:32 [entrez]
PHST- 2023/09/16 00:00 [pmc-release]
AID - molecules28186655 [pii]
AID - molecules-28-06655 [pii]
AID - 10.3390/molecules28186655 [doi]
PST - epublish
SO  - Molecules. 2023 Sep 16;28(18):6655. doi: 10.3390/molecules28186655.