PMID- 37764859
OWN - NLM
STAT- MEDLINE
DCOM- 20230929
LR  - 20231003
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 15
IP  - 18
DP  - 2023 Sep 20
TI  - Caloric Restriction: A Novel Conditioning Strategy to Improve the Survival of 
      Ischemically Challenged Musculocutaneous Random Pattern Flaps.
LID - 10.3390/nu15184076 [doi]
LID - 4076
AB  - Caloric restriction (CR) is a cost-effective and easy-to-perform approach to 
      counteracting surgical stress. The present study therefore evaluates the 
      tissue-protective effects of a 30% CR in musculocutaneous flaps undergoing 
      ischemia. For this purpose, a well-established murine dorsal skinfold chamber 
      model, in combination with random pattern musculocutaneous flaps, was used. 
      C57BL/6N mice were divided at random into a CR group (n = 8) and a control group 
      with unrestricted access to standard chow (n = 8). The CR animals were subjected 
      to a 30% reduction in caloric intake for 10 days before flap elevation. 
      Intravital fluorescence microscopy was carried out on days 1, 3, 5, 7 and 10 
      after flap elevation to assess the nutritive blood perfusion, angiogenesis and 
      flap necrosis. Subsequently, the flap tissue was harvested for additional 
      histological and immunohistochemical analyses. The CR-treated animals exhibited a 
      significantly higher functional capillary density and more newly formed 
      microvessels within the flap tissue when compared to the controls; this was 
      associated with a significantly higher flap survival rate. Immunohistochemical 
      analyses showed a decreased invasion of myeloperoxidase-positive neutrophilic 
      granulocytes into the flap tissue of the CR-treated mice. Moreover, the detection 
      of cleaved caspase-3 revealed fewer cells undergoing apoptosis in the transition 
      zone between the vital and necrotic tissue in the flaps of the CR-treated mice. 
      These results demonstrate that a CR of 30% effectively prevents flap necrosis by 
      maintaining microperfusion on a capillary level and inhibiting inflammation under 
      ischemic stress. Hence, CR represents a promising novel conditioning strategy for 
      improving the survival of musculocutaneous flaps with random pattern perfusion.
FAU - Weinzierl, Andrea
AU  - Weinzierl A
AUID- ORCID: 0000-0003-2449-2698
AD  - Institute for Clinical and Experimental Surgery, Saarland University, 66421 
      Homburg, Germany.
AD  - Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, 8091 
      Zurich, Switzerland.
FAU - Coerper, Maximilian
AU  - Coerper M
AD  - Institute for Clinical and Experimental Surgery, Saarland University, 66421 
      Homburg, Germany.
FAU - Harder, Yves
AU  - Harder Y
AUID- ORCID: 0000-0002-2557-249X
AD  - Department of Plastic, Reconstructive and Aesthetic Surgery, Ospedale Regionale 
      di Lugano, Ente Ospedaliero Cantonale (EOC), 6900 Lugano, Switzerland.
AD  - Faculty of Biomedical Sciences, Universita della Svizzera Italiana, 6900 Lugano, 
      Switzerland.
FAU - Menger, Michael D
AU  - Menger MD
AUID- ORCID: 0000-0002-9670-7961
AD  - Institute for Clinical and Experimental Surgery, Saarland University, 66421 
      Homburg, Germany.
FAU - Laschke, Matthias W
AU  - Laschke MW
AUID- ORCID: 0000-0002-7847-8456
AD  - Institute for Clinical and Experimental Surgery, Saarland University, 66421 
      Homburg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20230920
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
SB  - IM
MH  - Animals
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Caloric Restriction
MH  - *Energy Intake
MH  - Apoptosis
MH  - Necrosis
PMC - PMC10536342
OTO - NOTNLM
OT  - angiogenesis
OT  - caloric restriction
OT  - dietary restriction
OT  - dorsal skinfold chamber
OT  - intravital fluorescence microscopy
OT  - microcirculation
OT  - necrosis
OT  - nutrition
OT  - random pattern flap
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/28 06:42
MHDA- 2023/09/29 06:44
PMCR- 2023/09/20
CRDT- 2023/09/28 01:36
PHST- 2023/08/26 00:00 [received]
PHST- 2023/09/14 00:00 [revised]
PHST- 2023/09/19 00:00 [accepted]
PHST- 2023/09/29 06:44 [medline]
PHST- 2023/09/28 06:42 [pubmed]
PHST- 2023/09/28 01:36 [entrez]
PHST- 2023/09/20 00:00 [pmc-release]
AID - nu15184076 [pii]
AID - nutrients-15-04076 [pii]
AID - 10.3390/nu15184076 [doi]
PST - epublish
SO  - Nutrients. 2023 Sep 20;15(18):4076. doi: 10.3390/nu15184076.