PMID- 37770504
OWN - NLM
STAT- MEDLINE
DCOM- 20231002
LR  - 20231121
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Sep 28
TI  - Diminishing of Helicobacter pylori adhesion to Cavia porcellus gastric epithelial 
      cells by BCG vaccine mycobacteria.
PG  - 16305
LID - 10.1038/s41598-023-43571-3 [doi]
LID - 16305
AB  - Mycobacterium bovis onco-BCG bacilli used in immunotherapy of bladder cancer are 
      candidates for training of immune cells towards microbial pathogens. Increasing 
      antibiotic resistance of gastric pathogen Helicobacter pylori (Hp) prompts the 
      search for new anti-Hp and immunomodulatory formulations. Colonization of gastric 
      mucosa by Hp through mucin 5 AC (MUC5AC) ligands could potentially be a 
      therapeutic target. The aim of this study was to examine the ability of onco-BCG 
      mycobacteria to reduce Hp adhesion to gastric epithelial cells using Cavia 
      porcellus model. Animals were inoculated per os with 0.85% NaCl, Hp alone, 
      onco-BCG alone or with onco-BCG and Hp. After 7/28 days Mucin5AC and Hp binding 
      to gastric epithelium were assessed in gastric tissue specimens by staining with 
      anti-Mucin5AC and anti-Hp antibodies, respectively, both fluorescently labeled. 
      Primary gastric epithelial cells were treated ex vivo with live Hp or Hp surface 
      antigens (glycine extract or lipopolysaccharide) alone or with onco-BCG. In such 
      cells MUC5AC and Hp binding were determined as above. Mycobacteria reduced the 
      amount of MUC5AC animals infected with Hp and in gastric epithelial cells pulsed 
      in vitro with Hp components. Decrease of MUC5AC driven in cell cultures in vitro 
      and in gastric tissue exposed ex vivo to mycobacteria was related to diminished 
      adhesion of H. pylori bacilli. Vaccine mycobacteria by diminishing the amount of 
      MUC5AC in gastric epithelial cells may reduce Hp adhesion.
CI  - (c) 2023. Springer Nature Limited.
FAU - Gonciarz, Weronika
AU  - Gonciarz W
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Banacha 12-16, 90-237, Lodz, Poland. 
      weronika.gonciarz@biol.uni.lodz.pl.
FAU - Chyb, Maciej
AU  - Chyb M
AD  - Department of Molecular Microbiology, Faculty of Biology and Environmental 
      Protection, University of Lodz, Lodz, Poland.
AD  - Bio-Med-Chem Doctoral School of the University of Lodz and Lodz Institutes of the 
      Polish Academy of Sciences, Lodz, Poland.
FAU - Chmiela, Magdalena
AU  - Chmiela M
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Banacha 12-16, 90-237, Lodz, Poland. 
      magdalena.chmiela@biol.uni.lodz.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230928
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (BCG Vaccine)
SB  - IM
MH  - Guinea Pigs
MH  - Animals
MH  - *Helicobacter pylori
MH  - BCG Vaccine/therapeutic use
MH  - Stomach
MH  - Gastric Mucosa/metabolism
MH  - Epithelial Cells/metabolism
MH  - *Mycobacterium
MH  - *Helicobacter Infections/microbiology
PMC - PMC10539345
COIS- The authors declare no competing interests.
EDAT- 2023/09/29 00:42
MHDA- 2023/10/02 06:42
PMCR- 2023/09/28
CRDT- 2023/09/28 23:22
PHST- 2023/06/21 00:00 [received]
PHST- 2023/09/26 00:00 [accepted]
PHST- 2023/10/02 06:42 [medline]
PHST- 2023/09/29 00:42 [pubmed]
PHST- 2023/09/28 23:22 [entrez]
PHST- 2023/09/28 00:00 [pmc-release]
AID - 10.1038/s41598-023-43571-3 [pii]
AID - 43571 [pii]
AID - 10.1038/s41598-023-43571-3 [doi]
PST - epublish
SO  - Sci Rep. 2023 Sep 28;13(1):16305. doi: 10.1038/s41598-023-43571-3.