PMID- 37771817
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231003
IS  - 2667-0119 (Electronic)
IS  - 2667-0119 (Linking)
VI  - 5
DP  - 2023 Dec 15
TI  - Identification and functional characterization of Interleukin-11 in goldfish 
      (Carassius auratus L.).
PG  - 100117
LID - 10.1016/j.fsirep.2023.100117 [doi]
LID - 100117
AB  - Interleukin-11 (IL-11) is a versatile cytokine that modulates cellular 
      differentiation and proliferation in various cell types and tissues. In this 
      study, IL-11 gene from goldfish (Carassius auratus L.) has been identified and 
      characterized. Goldfish IL-11 (gfIL-11) has an open reading frame (ORF) that 
      spans 591 base pairs (bp). The ORF encodes a precursor protein consisting of 196 
      amino acids (aa), which includes a 26 aa signal peptide and a conserved domain 
      belonging to the IL-11 superfamily. Based on phylogenetic analysis, gfIL-11 was 
      found to be closely related to other IL-11 homologues identified in various fish 
      species. The gfIL-11 transcript exhibited varied expression levels across all the 
      analyzed tissues, with the highest expression observed in the gill and spleen. 
      Treatment of goldfish head kidney leukocytes (HKLs) with LPS and live Aeromonas 
      hydrophila, increased gfIL-11 mRNA expression level. Recombinant gfIL-11 protein 
      (rgIL-11) induced a dose-dependent production of TNF-alpha and IFNgamma from goldfish 
      HKLs. Furthermore, the administration of rgIL-11 to goldfish HKLs triggered an 
      increase in the expression of various transcription factors such as MafB, cJun, 
      GATA2, and Egr1, which play a vital role in the differentiation of myeloid 
      precursors into macrophages and monocytes. Our findings provide evidence that 
      IL-11 is a crucial cytokine that promotes cell proliferation, immune response, 
      and differentiation across various hematopoietic lineages and stages of goldfish.
CI  - (c) 2023 The Author(s).
FAU - Gouife, Moussa
AU  - Gouife M
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Zhu, Songwei
AU  - Zhu S
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Huang, Kejing
AU  - Huang K
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Nawaz, Mateen
AU  - Nawaz M
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Yue, Xinyuan
AU  - Yue X
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Ma, Rongrong
AU  - Ma R
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
AD  - Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
AD  - Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy 
      Aquaculture, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Jiang, Jianhu
AU  - Jiang J
AD  - Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang 313001, China.
FAU - Zhou, Suming
AU  - Zhou S
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
AD  - Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
AD  - Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy 
      Aquaculture, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Xie, Jiasong
AU  - Xie J
AD  - School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
AD  - Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
AD  - Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy 
      Aquaculture, Ningbo University, Ningbo, Zhejiang 315211, China.
LA  - eng
PT  - Journal Article
DEP - 20230918
PL  - England
TA  - Fish Shellfish Immunol Rep
JT  - Fish and shellfish immunology reports
JID - 9918486785306676
PMC - PMC10523422
OTO - NOTNLM
OT  - Cytokine
OT  - Fish
OT  - Hematopoiesis
OT  - IL-11
OT  - Progenitors
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/09/29 06:44
MHDA- 2023/09/29 06:45
PMCR- 2023/09/18
CRDT- 2023/09/29 03:56
PHST- 2023/08/04 00:00 [received]
PHST- 2023/09/17 00:00 [revised]
PHST- 2023/09/17 00:00 [accepted]
PHST- 2023/09/29 06:45 [medline]
PHST- 2023/09/29 06:44 [pubmed]
PHST- 2023/09/29 03:56 [entrez]
PHST- 2023/09/18 00:00 [pmc-release]
AID - S2667-0119(23)00037-3 [pii]
AID - 100117 [pii]
AID - 10.1016/j.fsirep.2023.100117 [doi]
PST - epublish
SO  - Fish Shellfish Immunol Rep. 2023 Sep 18;5:100117. doi: 
      10.1016/j.fsirep.2023.100117. eCollection 2023 Dec 15.