PMID- 37774941
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240131
IS  - 1095-9319 (Electronic)
IS  - 0026-2862 (Linking)
VI  - 151
DP  - 2024 Jan
TI  - Astrocytic expression of Yes-associated protein (YAP) regulates retinal 
      neovascularization in a mouse model of oxygen-induced retinopathy.
PG  - 104611
LID - S0026-2862(23)00137-1 [pii]
LID - 10.1016/j.mvr.2023.104611 [doi]
AB  - Pathological neovascularization is the hallmark of many vascular oculopathies. 
      There is still a great deal of uncertainty surrounding retinal neovascularization 
      research. A working hypothesis that astrocytic Yes-associated protein (YAP) act 
      as a key factor in retinal neovascularization was proposed. And our study was 
      conducted to verified this hypothesis. In vivo, we successfully generated mice 
      deficient in YAP in astrocytes (YAP(f/f) GFAP-Cre mice) and set up oxygen-induced 
      retinopathy (OIR) model. Pathological neovascularization was evaluated by 
      immunofluorescence staining and western blotting. In vitro, cultured retinal 
      astrocytes were transfected with YAP siRNA. Enzyme-linked immunosorbent assay 
      (ELISA) and western blot were used to determine the proteins in the supernatants 
      and cells. The results showed that YAP was upregulated and activated in the OIR 
      mice retinas. Conditional ablation of YAP aggravated pathological 
      neovascularization, along with the upregulation of vascular endothelial growth 
      factor A (VEGF-A) and monocyte chemoattractant protein-1 (MCP-1). Studies in 
      vitro confirmed that the knockdown of YAP in astrocytes lead to increases in 
      VEGF-A and MCP-1 levels, thus enhancing pro-angiogenic capability of YAP-deficit 
      astrocytes. In conclusion, astrocytic YAP alleviates retinal pathological 
      angiogenesis by inhibiting the over-activation of astrocytes, which suppresses 
      excessive VEGF-A production and neuroinflammation.
CI  - Copyright (c) 2023. Published by Elsevier Inc.
FAU - Liu, Zhifei
AU  - Liu Z
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China.
FAU - Zhang, Jieqiong
AU  - Zhang J
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China.
FAU - Li, Xue
AU  - Li X
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China.
FAU - Hu, Qiumei
AU  - Hu Q
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China.
FAU - Chen, Xi
AU  - Chen X
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China.
FAU - Luo, Linlin
AU  - Luo L
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China.
FAU - Ai, Liqianyu
AU  - Ai L
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China. Electronic 
      address: echoqian@tmmu.edu.cn.
FAU - Ye, Jian
AU  - Ye J
AD  - Department of Ophthalmology, Research Institute of Surgery & Daping Hospital, 
      Army Medical Center of PLA, Army Medical University, Chongqing, China. Electronic 
      address: yejian1979@tmmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230928
PL  - United States
TA  - Microvasc Res
JT  - Microvascular research
JID - 0165035
RN  - S88TT14065 (Oxygen)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (YAP-Signaling Proteins)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Retinal Neovascularization/metabolism
MH  - Oxygen/toxicity/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - Neovascularization, Pathologic/metabolism
MH  - YAP-Signaling Proteins
MH  - Astrocytes/pathology
MH  - Mice, Inbred C57BL
MH  - Disease Models, Animal
MH  - Animals, Newborn
OTO - NOTNLM
OT  - Astrocytes
OT  - Neovascularization
OT  - Oxygen-induced retinopathy
OT  - YAP
COIS- Declaration of competing interest The authors declare that they have no competing 
      interests.
EDAT- 2023/09/30 09:42
MHDA- 2023/12/17 09:43
CRDT- 2023/09/29 19:16
PHST- 2023/03/29 00:00 [received]
PHST- 2023/09/19 00:00 [revised]
PHST- 2023/09/19 00:00 [accepted]
PHST- 2023/12/17 09:43 [medline]
PHST- 2023/09/30 09:42 [pubmed]
PHST- 2023/09/29 19:16 [entrez]
AID - S0026-2862(23)00137-1 [pii]
AID - 10.1016/j.mvr.2023.104611 [doi]
PST - ppublish
SO  - Microvasc Res. 2024 Jan;151:104611. doi: 10.1016/j.mvr.2023.104611. Epub 2023 Sep 
      28.