PMID- 37777021
OWN - NLM
STAT- MEDLINE
DCOM- 20231206
LR  - 20231216
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 72
DP  - 2023 Dec
TI  - Cancer-associated fibroblasts secret extracellular vesicles to support cell 
      proliferation and epithelial-mesenchymal transition in laryngeal squamous cell 
      carcinoma.
PG  - 101934
LID - S0890-8508(23)00043-9 [pii]
LID - 10.1016/j.mcp.2023.101934 [doi]
AB  - As the critical components of tumor microenvironment, cancer-associated 
      fibroblasts (CAFs) support the development of various type of cancers, including 
      laryngeal squamous cell carcinoma (LSCC), but the detailed molecular mechanisms 
      by which cancer-associated fibroblasts interact with LSCC cells to facilitate its 
      progression have not been fully uncovered. In the present study, by analyzing the 
      contents from normal fibroblasts (NFs) and cancer-associated fibroblasts-derived 
      extracellular vesicles (EVs) with Real-Time qPCR analysis, we found that the 
      tumor-initiating LncRNA TUC338 was significantly upregulated in the 
      cancer-associated fibroblasts-derived extracellular vesicles, compared to the 
      normal fibroblasts-secreted extracellular vesicles. Further experiments confirmed 
      that cancer-associated fibroblasts-derived extracellular vesicles promoted cell 
      proliferation, colony formation abilities, epithelial-mesenchymal transition 
      (EMT) and tumorigenesis of LSCC cells via delivering LncRNA TUC338. The 
      mechanical experiments verified that LncRNA TUC338 was stabilized by 
      METTL3/YTHDF1-mediated N6-methyladenosine (m6A) modifications, and elevated 
      LncRNA TUC338 sponged miR-8485 to upregulate chromobox homolog 2 (CBX2) in LSCC 
      cells in a competing endogenous RNA mechanisms-dependent manner. Moreover, our 
      rescue experiments evidenced that cancer-associated fibroblasts-derived LncRNA 
      TUC338-containing extracellular vesicles-induced supportive effects in LSCC 
      aggressiveness were all abrogated by overexpressing miR-8485 and silencing CBX2. 
      Collectively, this study is the first to identify a novel m6A/LncRNA 
      TUC338/miR-8485/CBX2 axis in CAFs-EVs-mediated LSCC development, and to show its 
      potential as a diagnostic biomarker for LSCC.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Li, Tingting
AU  - Li T
AD  - Department of Otolaryngology-Head and Neck Surgery, The Fourth Affiliated 
      Hospital of Harbin Medical University, Yiyuan Street No. 37, Nangang District, 
      Harbin, 150001, Heilongjiang, China. Electronic address: 120001998@hrbmu.edu.cn.
FAU - Tian, Linli
AU  - Tian L
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Xuefu Road No. 246, Nangang District, 
      Harbin, 150086, Heilongjiang, China. Electronic address: Tianll78@163.com.
FAU - Cao, Jing
AU  - Cao J
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Xuefu Road No. 246, Nangang District, 
      Harbin, 150086, Heilongjiang, China. Electronic address: Caoj001989@163.com.
FAU - Liu, Ming
AU  - Liu M
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Xuefu Road No. 246, Nangang District, 
      Harbin, 150086, Heilongjiang, China. Electronic address: lmdoctor@hrbmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231009
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.1.1.62 (METTL3 protein, human)
RN  - EC 2.1.1.- (Methyltransferases)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - *Cancer-Associated Fibroblasts/metabolism
MH  - Cell Line, Tumor
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Cell Proliferation/genetics
MH  - *Extracellular Vesicles/metabolism
MH  - *Head and Neck Neoplasms/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Tumor Microenvironment
MH  - Methyltransferases/genetics/metabolism
OTO - NOTNLM
OT  - CBX2
OT  - Cancer-associated fibroblasts
OT  - Competing endogenous RNA
OT  - Extracellular vesicles
OT  - LncRNA TUC338
EDAT- 2023/10/01 04:44
MHDA- 2023/12/06 06:42
CRDT- 2023/09/30 19:14
PHST- 2023/05/18 00:00 [received]
PHST- 2023/09/28 00:00 [revised]
PHST- 2023/09/28 00:00 [accepted]
PHST- 2023/12/06 06:42 [medline]
PHST- 2023/10/01 04:44 [pubmed]
PHST- 2023/09/30 19:14 [entrez]
AID - S0890-8508(23)00043-9 [pii]
AID - 10.1016/j.mcp.2023.101934 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2023 Dec;72:101934. doi: 10.1016/j.mcp.2023.101934. Epub 2023 
      Oct 9.