PMID- 37780223
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231003
IS  - 1976-1457 (Print)
IS  - 2005-6168 (Electronic)
IS  - 1976-1457 (Linking)
VI  - 17
IP  - 5
DP  - 2023 Oct
TI  - Protective effect of Lycium barbarum leaf extracts on atopic dermatitis: in vitro 
      and in vivo studies.
PG  - 855-869
LID - 10.4162/nrp.2023.17.5.855 [doi]
AB  - BACKGROUND/OBJECTIVES: Atopic dermatitis (AD) is a chronic disease with an 
      increasing incidence globally; therefore, there is a growing demand for natural 
      compounds effective in treating dermatitis. In this study, the protective effects 
      of Lycium barbarum leaves with and without chlorophyll (LLE and LLE[Ch-]) on AD 
      were investigated in animal models of AD and HaCaT cells. Further, we 
      investigated whether LLE and LLE(Ch-) show any differences in physiological 
      activity. MATERIALS/METHODS: AD was induced by 2,4-dinitrochlorobenzene (DNCB) 
      for three weeks, while NC/Nga mice were fed LLE or LLE(Ch-) extracts for 7 weeks. 
      Serum immunoglobulin E (IgE) and cytokine (tumor necrosis factor [TNF]-alpha, 
      interleukin [IL]-6, and IL-4) concentrations and the degree of DNA fragmentation 
      in lymphocytes were examined. A histopathological examination (haematoxylin & 
      eosin staining and blue spots of toluidine) of the dorsal skin of mice was 
      performed. To elucidate the mechanism of action, the expression of the thymus and 
      activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) were 
      measured in HaCaT cells. RESULTS: Serum IgE and cytokines (TNF-alpha and IL-6) levels 
      as well as DNA fragmentation of lymphocytes were significantly decreased in 
      AD-induced mice treated with LLE or LLE(Ch-) compared to those of the control 
      group. The epidermal thickness of the dorsal skin and mast cell infiltration in 
      the LLE group significantly reduced compared to that in the control group. The 
      LLE extracts showed no cytotoxicity up to 1,000 microg/mL in HaCaT cells. LLE or 
      LLE(Ch-)-treated group showed a reduction of TARC and MDC in TNF-alpha-and 
      IFN-gamma-stimulated HaCaT cells. CONCLUSIONS: These results suggest that LLE 
      potentially improves inflammation by reducing the expression of chemokines that 
      inhibit T helper 2 cell migration. LLE(Ch-) showed similar effects to LLE on 
      blood levels of IgE, TNF-alpha and IL-6 and protein expression in HaCat cells, but 
      the ultimate effect of skin improvement was not statistically significant. 
      Therefore, both LLE and LLE(Ch-) can be used as functional materials to alleviate 
      AD, but LLE(Ch-) appears to require more research to improve inflammation.
CI  - (c)2023 The Korean Nutrition Society and the Korean Society of Community Nutrition.
FAU - Lee, Han Sol
AU  - Lee HS
AUID- ORCID: 0000-0003-2649-3766
AD  - Department of Food and Nutrition, Chungnam National University, Daejeon 34134, 
      Korea.
FAU - Bae, Eun Young
AU  - Bae EY
AUID- ORCID: 0000-0003-1844-1296
AD  - Department of Food and Nutrition, Chungnam National University, Daejeon 34134, 
      Korea.
FAU - Ly, Sun Yung
AU  - Ly SY
AUID- ORCID: 0000-0001-6813-8573
AD  - Department of Food and Nutrition, Chungnam National University, Daejeon 34134, 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20230711
PL  - Korea (South)
TA  - Nutr Res Pract
JT  - Nutrition research and practice
JID - 101311052
PMC - PMC10522814
OTO - NOTNLM
OT  - Lycium barbarum
OT  - atopic dermatitis
OT  - chlorophyll
COIS- Conflict of Interest: The authors declare no potential conflicts of interests.
EDAT- 2023/10/02 06:42
MHDA- 2023/10/02 06:43
PMCR- 2023/10/01
CRDT- 2023/10/02 04:25
PHST- 2022/09/14 00:00 [received]
PHST- 2023/01/30 00:00 [revised]
PHST- 2023/06/30 00:00 [accepted]
PHST- 2023/10/02 06:43 [medline]
PHST- 2023/10/02 06:42 [pubmed]
PHST- 2023/10/02 04:25 [entrez]
PHST- 2023/10/01 00:00 [pmc-release]
AID - 10.4162/nrp.2023.17.5.855 [doi]
PST - ppublish
SO  - Nutr Res Pract. 2023 Oct;17(5):855-869. doi: 10.4162/nrp.2023.17.5.855. Epub 2023 
      Jul 11.