PMID- 37781121
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231003
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 10
DP  - 2023
TI  - Extracellular thiamine concentration influences thermogenic competency of 
      differentiating neck area-derived human adipocytes.
PG  - 1207394
LID - 10.3389/fnut.2023.1207394 [doi]
LID - 1207394
AB  - INTRODUCTION: Brown adipose tissue (BAT) dissipates energy in the form of heat 
      majorly via the mitochondrial uncoupling protein 1 (UCP1). The activation of BAT, 
      which is enriched in the neck area and contains brown and beige adipocytes in 
      humans, was considered as a potential therapeutic target to treat obesity. 
      Therefore, finding novel agents that can stimulate the differentiation and 
      recruitment of brown or beige thermogenic adipocytes are important subjects for 
      investigation. The current study investigated how the availability of 
      extracellular thiamine (vitamin B1), an essential cofactor of mitochondrial 
      enzyme complexes that catalyze key steps in the catabolism of nutrients, affects 
      the expression of thermogenic marker genes and proteins and subsequent functional 
      parameters during ex vivo adipocyte differentiation. METHODS: We differentiated 
      primary human adipogenic progenitors that were cultivated from subcutaneous (SC) 
      or deep neck (DN) adipose tissues in the presence of gradually increasing 
      thiamine concentrations during their 14-day differentiation program. mRNA and 
      protein expression of thermogenic genes were analyzed by RT-qPCR and western 
      blot, respectively. Cellular respiration including stimulated maximal and 
      proton-leak respiration was measured by Seahorse analysis. RESULTS: Higher 
      thiamine levels resulted in increased expression of thiamine transporter 1 and 2 
      both at mRNA and protein levels in human neck area-derived adipocytes. Gradually 
      increasing concentrations of thiamine led to increased basal, cAMP-stimulated, 
      and proton-leak respiration along with elevated mitochondrial biogenesis of the 
      differentiated adipocytes. The extracellular thiamine availability during 
      adipogenesis determined the expression levels of UCP1, PGC1a, CKMT2, and other 
      browning-related genes and proteins in primary SC and DN-derived adipocytes in a 
      concentration-dependent manner. Providing abundant amounts of thiamine further 
      increased the thermogenic competency of the adipocytes. DISCUSSION: Case studies 
      in humans reported that thiamine deficiency was found in patients with type 2 
      diabetes and obesity. Our study raises the possibility of a novel strategy with 
      long-term thiamine supplementation, which can enhance the thermogenic competency 
      of differentiating neck area-derived adipocytes for preventing or combating 
      obesity.
CI  - Copyright (c) 2023 Vinnai, Arianti, Gyory, Bacso, Fesus and Kristof.
FAU - Vinnai, Boglarka Agnes
AU  - Vinnai BA
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, University 
      of Debrecen, Debrecen, Hungary.
AD  - Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, 
      Debrecen, Hungary.
FAU - Arianti, Rini
AU  - Arianti R
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, University 
      of Debrecen, Debrecen, Hungary.
AD  - Universitas Muhammadiyah Bangka Belitung, Pangkalanbaru, Indonesia.
FAU - Gyory, Ferenc
AU  - Gyory F
AD  - Department of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
FAU - Bacso, Zsolt
AU  - Bacso Z
AD  - Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, 
      Debrecen, Hungary.
AD  - Department of Biophysics and Cell Biology, Faculty of Medicine, University of 
      Debrecen, Debrecen, Hungary.
AD  - Faculty of Pharmacy, University of Debrecen, Debrecen, Hungary.
FAU - Fesus, Laszlo
AU  - Fesus L
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, University 
      of Debrecen, Debrecen, Hungary.
FAU - Kristof, Endre
AU  - Kristof E
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, University 
      of Debrecen, Debrecen, Hungary.
LA  - eng
PT  - Journal Article
DEP - 20230913
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC10534038
OTO - NOTNLM
OT  - UCP1
OT  - adipocytes
OT  - beige
OT  - obesity
OT  - thermogenesis
OT  - thiamine
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/10/02 06:42
MHDA- 2023/10/02 06:43
PMCR- 2023/01/01
CRDT- 2023/10/02 04:39
PHST- 2023/04/17 00:00 [received]
PHST- 2023/08/14 00:00 [accepted]
PHST- 2023/10/02 06:43 [medline]
PHST- 2023/10/02 06:42 [pubmed]
PHST- 2023/10/02 04:39 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2023.1207394 [doi]
PST - epublish
SO  - Front Nutr. 2023 Sep 13;10:1207394. doi: 10.3389/fnut.2023.1207394. eCollection 
      2023.