PMID- 37784079
OWN - NLM
STAT- MEDLINE
DCOM- 20231004
LR  - 20231123
IS  - 1471-2253 (Electronic)
IS  - 1471-2253 (Linking)
VI  - 23
IP  - 1
DP  - 2023 Oct 2
TI  - Dexmedetomidine modulates neuronal activity of horizontal limbs of diagonal band 
      via alpha2 adrenergic receptor in mice.
PG  - 327
LID - 10.1186/s12871-023-02278-8 [doi]
LID - 327
AB  - BACKGROUND AND OBJECTIVES: Dexmedetomidine (DEX) is widely used in clinical 
      sedation which has little effect on cardiopulmonary inhibition, however the 
      mechanism remains to be elucidated. The basal forebrain (BF) is a key nucleus 
      that controls sleep-wake cycle. The horizontal limbs of diagonal bundle (HDB) is 
      one subregions of the BF. The purpose of this study was to examine whether the 
      possible mechanism of DEX is through the alpha2 adrenergic receptor of BF (HDB). 
      METHODS: In this study, we investigated the effects of DEX on the BF (HDB) by 
      using whole cell patch clamp recordings. The threshold stimulus intensity, the 
      inter-spike-intervals (ISIs) and the frequency of action potential firing in the 
      BF (HDB) neurons were recorded by application of DEX (2 microM) and co-application of 
      a alpha(2) adrenergic receptor antagonist phentolamine (PHEN) (10 microM). RESULTS: DEX 
      (2 microM) increased the threshold stimulus intensity, inhibited the frequency of 
      action potential firing and enlarged the inter-spike-interval (ISI) in the BF 
      (HDB) neurons. These effects were reversed by co-application of PHEN (10 microM). 
      CONCLUSION: Taken together, our findings revealed DEX decreased the discharge 
      activity of BF (HDB) neuron via alpha(2) adrenergic receptors.
CI  - (c) 2023. BioMed Central Ltd., part of Springer Nature.
FAU - Zhang, Xia-Wei
AU  - Zhang XW
AD  - Department of Physiology, School of Basic Medical Sciences, Anhui Medical 
      University, 230032, Hefei, China.
FAU - Chen, Lei
AU  - Chen L
AD  - Departments of Pharmacy, The First Affiliated Hospital of Anhui University of 
      Chinese Medicine, 230031, Hefei, China.
FAU - Chen, Chang-Feng
AU  - Chen CF
AD  - Department of Physiology, School of Basic Medical Sciences, Anhui Medical 
      University, 230032, Hefei, China.
FAU - Cheng, Juan
AU  - Cheng J
AD  - Department of Physiology, School of Basic Medical Sciences, Anhui Medical 
      University, 230032, Hefei, China.
FAU - Zhang, Ping-Ping
AU  - Zhang PP
AD  - Department of Physiology, School of Basic Medical Sciences, Anhui Medical 
      University, 230032, Hefei, China.
FAU - Wang, Lie-Cheng
AU  - Wang LC
AD  - Department of Physiology, School of Basic Medical Sciences, Anhui Medical 
      University, 230032, Hefei, China. wangliecheng@ahmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231002
PL  - England
TA  - BMC Anesthesiol
JT  - BMC anesthesiology
JID - 100968535
RN  - 67VB76HONO (Dexmedetomidine)
RN  - 0 (Receptors, Adrenergic, alpha-2)
RN  - 0 (Adrenergic alpha-2 Receptor Agonists)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Dexmedetomidine/pharmacology
MH  - Receptors, Adrenergic, alpha-2
MH  - Signal Transduction
MH  - Neurons
MH  - Adrenergic alpha-2 Receptor Agonists/pharmacology
PMC - PMC10544551
OTO - NOTNLM
OT  - Basal forebrain
OT  - Dexmedetomidine
OT  - Horizontal limbs of diagonal bundle
OT  - alpha2 adrenergic receptor
COIS- The authors declare no competing fnancial interests.
EDAT- 2023/10/03 00:42
MHDA- 2023/10/04 06:44
PMCR- 2023/10/02
CRDT- 2023/10/02 23:46
PHST- 2023/04/24 00:00 [received]
PHST- 2023/09/11 00:00 [accepted]
PHST- 2023/10/04 06:44 [medline]
PHST- 2023/10/03 00:42 [pubmed]
PHST- 2023/10/02 23:46 [entrez]
PHST- 2023/10/02 00:00 [pmc-release]
AID - 10.1186/s12871-023-02278-8 [pii]
AID - 2278 [pii]
AID - 10.1186/s12871-023-02278-8 [doi]
PST - epublish
SO  - BMC Anesthesiol. 2023 Oct 2;23(1):327. doi: 10.1186/s12871-023-02278-8.