PMID- 37786323
OWN - NLM
STAT- MEDLINE
DCOM- 20231110
LR  - 20231117
IS  - 1364-3703 (Electronic)
IS  - 1464-6722 (Print)
IS  - 1364-3703 (Linking)
VI  - 24
IP  - 12
DP  - 2023 Dec
TI  - The interaction of two Puccinia striiformis f. sp. tritici effectors modulates 
      high-temperature seedling-plant resistance in wheat.
PG  - 1522-1534
LID - 10.1111/mpp.13390 [doi]
AB  - Wheat cultivar Xiaoyan 6 (XY6) has high-temperature seedling-plant (HTSP) 
      resistance to Puccinia striiformis f. sp. tritici (Pst). However, the molecular 
      mechanism of Pst effectors involved in HTSP resistance remains unclear. In this 
      study, we determined the interaction between two Pst effectors, PstCEP1 and 
      PSTG_11208, through yeast two-hybrid (Y2H), bimolecular fluorescence 
      complementation (BiFC), and pull-down assays. Transient overexpression of 
      PSTG_11208 enhanced HTSP resistance in different temperature treatments. The 
      interaction between PstCEP1 and PSTG_11208 inhibited the resistance enhancement 
      by PSTG_11208. Furthermore, the wheat apoplastic thaumatin-like protein 1 
      (TaTLP1) appeared to recognize Pst invasion by interacting with PSTG_11208 and 
      initiate the downstream defence response by the pathogenesis-related protein 
      TaPR1. Silencing of TaTLP1 and TaPR1 separately or simultaneously reduced HTSP 
      resistance to Pst in XY6. Moreover, we found that PstCEP1 targeted wheat 
      ferredoxin 1 (TaFd1), a homologous protein of rice OsFd1. Silencing of TaFd1 
      affected the stability of photosynthesis in wheat plants, resulting in chlorosis 
      on the leaves and reducing HTSP resistance. Our findings revealed the synergistic 
      mechanism of effector proteins in the process of pathogen infection.
CI  - (c) 2023 The Authors. Molecular Plant Pathology published by British Society for 
      Plant Pathology and John Wiley & Sons Ltd.
FAU - Bao, Xiyue
AU  - Bao X
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, China.
FAU - Hu, Yangshan
AU  - Hu Y
AUID- ORCID: 0000-0003-3651-4540
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, China.
AD  - State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, 
      Yunnan Agricultural University, Kunming, Yunnan, China.
FAU - Li, Yuxiang
AU  - Li Y
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, China.
FAU - Chen, Xianming
AU  - Chen X
AUID- ORCID: 0000-0002-9013-5974
AD  - Agricultural Research Service, United States Department of Agriculture and 
      Department of Plant Pathology, Washington State University, Pullman, Washington, 
      USA.
FAU - Shang, Hongsheng
AU  - Shang H
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, China.
FAU - Hu, Xiaoping
AU  - Hu X
AUID- ORCID: 0000-0001-8265-8460
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231002
PL  - England
TA  - Mol Plant Pathol
JT  - Molecular plant pathology
JID - 100954969
RN  - Puccinia striiformis
SB  - IM
MH  - *Seedlings/metabolism
MH  - Triticum/genetics/metabolism
MH  - Temperature
MH  - Puccinia
MH  - *Basidiomycota/physiology
MH  - Plant Diseases
PMC - PMC10632793
OTO - NOTNLM
OT  - Puccinia striiformis f. sp. tritici
OT  - effector protein
OT  - high-temperature seedling-plant resistance
OT  - pathogenesis-related protein
OT  - wheat stripe rust
COIS- The authors declare no conflict of interest.
EDAT- 2023/10/03 06:46
MHDA- 2023/11/10 06:45
PMCR- 2023/10/02
CRDT- 2023/10/03 02:43
PHST- 2023/08/15 00:00 [revised]
PHST- 2023/05/17 00:00 [received]
PHST- 2023/08/31 00:00 [accepted]
PHST- 2023/11/10 06:45 [medline]
PHST- 2023/10/03 06:46 [pubmed]
PHST- 2023/10/03 02:43 [entrez]
PHST- 2023/10/02 00:00 [pmc-release]
AID - MPP13390 [pii]
AID - 10.1111/mpp.13390 [doi]
PST - ppublish
SO  - Mol Plant Pathol. 2023 Dec;24(12):1522-1534. doi: 10.1111/mpp.13390. Epub 2023 
      Oct 2.