PMID- 3778661
OWN - NLM
STAT- MEDLINE
DCOM- 19870106
LR  - 20190912
IS  - 0829-8211 (Print)
IS  - 0829-8211 (Linking)
VI  - 64
IP  - 9
DP  - 1986 Sep
TI  - Characterization of lithium-induced enzyme release from human polymorphonuclear 
      leukocytes.
PG  - 880-5
AB  - Lysozyme release from purified human polymorphonuclear leukocytes was found to be 
      uniquely enhanced by 2.5-20 mM LiCl. This effect was dose dependent and was not 
      detected when the media was supplemented with NaCl, KCl, MgCl2, or CaCl2. The 
      purified isotopes of Li+, 6Li, and 7Li were equally effective in enhancing 
      lysozyme release from the cells at 10 and 20 mM, but 6Li was more effective than 
      7Li at 5 mM. The enhancement of enzyme release in the presence of Li+ was 
      comparable to the enhancement observed in the presence of 
      N-formylmethionylleucylphenylalanine (fMLP). Addition of LiCl plus fMLP did not 
      result in lysozyme release in excess of each stimulant alone, except when the 
      cells were incubated with 20 mM 6Li + 10(-5) M fMLP. In addition, enzyme release 
      induced by these two agents could be further enhanced to the same degree by 
      addition of cytochalasin D to the incubation mixtures. While similarities between 
      enzyme release induced by LiCl and fMLP were detected, optimal stimulation of 
      enzyme release by Li+ was much more sensitive to inhibition by pertussis toxin 
      than was maximal fMLP stimulation. Therefore, the intracellular events altered by 
      Li+ and the peptide may share some metabolic steps, but they differ in their 
      sensitivity to alterations in cAMP metabolism.
FAU - Hart, D A
AU  - Hart DA
FAU - Groenewoud, Y
AU  - Groenewoud Y
FAU - Chamberland, S
AU  - Chamberland S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Virulence Factors, Bordetella)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9FN79X2M3F (Lithium)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Enzyme Activation/drug effects
MH  - Humans
MH  - Lithium/*pharmacology
MH  - Muramidase/*metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*enzymology
MH  - Pertussis Toxin
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
AID - 10.1139/o86-117 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 1986 Sep;64(9):880-5. doi: 10.1139/o86-117.