PMID- 37789240
OWN - NLM
STAT- MEDLINE
DCOM- 20231113
LR  - 20231122
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 394
IP  - 2
DP  - 2023 Nov
TI  - Impact of endogenous and exogenous nitrogen species on macrophage extracellular 
      trap (MET) formation by bone marrow-derived macrophages.
PG  - 361-377
LID - 10.1007/s00441-023-03832-z [doi]
AB  - Macrophage extracellular traps (METs) represent a novel defense mechanism in the 
      antimicrobial arsenal of macrophages. However, mechanisms of MET formation are 
      still poorly understood and this is at least partially due to the lack of 
      reliable and reproducible models. Thus, we aimed at establishing a protocol of 
      MET induction by bone marrow-derived macrophages (BMDMs) obtained from 
      cryopreserved and then thawed bone marrow (BM) mouse cells. We report that BMDMs 
      obtained in this way were morphologically (F4/80(+)) and functionally (expression 
      of inducible nitric oxide (NO) synthase and NO production) differentiated and 
      responded to various stimuli of bacterial (lipopolysaccharide, LPS), fungal 
      (zymosan) and chemical (PMA) origin. Importantly, BMDMs were successfully casting 
      METs composed of extracellular DNA (extDNA) serving as their backbone to which 
      proteins such as H2A.X histones and matrix metalloproteinase 9 (MMP-9) were 
      attached. In rendered 3D structure of METs, extDNA and protein components were 
      embedded in each other. Since studies had shown the involvement of oxygen species 
      in MET release, we aimed at studying if reactive nitrogen species (RNS) such as 
      NO are also involved in MET formation. By application of NOS inhibitor - L-NAME 
      or nitric oxide donor (SNAP), we studied the involvement of endogenous and 
      exogenous RNS in traps release. We demonstrated that L-NAME halted MET formation 
      upon stimulation with LPS while SNAP alone induced it. The latter phenomenon was 
      further enhanced in the presence of LPS. Taken together, our findings demonstrate 
      that BMDMs obtained from cryopreserved BM cells are capable of forming METs in an 
      RNS-dependent manner.
CI  - (c) 2023. The Author(s).
FAU - Drab, Dominika
AU  - Drab D
AD  - Laboratory of Experimental Hematology, Institute of Zoology and Biomedical 
      Research, Jagiellonian University, 30-387, Krakow, Poland.
AD  - Doctoral School of Exact and Natural Sciences, Jagiellonian University, Krakow, 
      Poland.
FAU - Santocki, Michal
AU  - Santocki M
AD  - Laboratory of Experimental Hematology, Institute of Zoology and Biomedical 
      Research, Jagiellonian University, 30-387, Krakow, Poland.
FAU - Opydo, Malgorzata
AU  - Opydo M
AD  - Laboratory of Experimental Hematology, Institute of Zoology and Biomedical 
      Research, Jagiellonian University, 30-387, Krakow, Poland.
FAU - Kolaczkowska, Elzbieta
AU  - Kolaczkowska E
AUID- ORCID: 0000-0002-3573-3584
AD  - Laboratory of Experimental Hematology, Institute of Zoology and Biomedical 
      Research, Jagiellonian University, 30-387, Krakow, Poland. 
      ela.kolaczkowska@uj.edu.pl.
LA  - eng
GR  - 2021/43/B/NZ6/00782/Narodowym Centrum Nauki/
PT  - Journal Article
DEP - 20231004
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Lipopolysaccharides)
RN  - N762921K75 (Nitrogen)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Extracellular Traps/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Nitrogen/metabolism
MH  - NG-Nitroarginine Methyl Ester/metabolism
MH  - Macrophages/metabolism
MH  - Nitric Oxide/metabolism
PMC - PMC10638184
OTO - NOTNLM
OT  - Bone marrow-derived macrophages
OT  - Innate immunity
OT  - Lipopolysaccharide
OT  - Macrophage extracellular traps
OT  - Nitric oxide
COIS- The authors declare no competing interests.
EDAT- 2023/10/04 00:42
MHDA- 2023/11/13 06:42
PMCR- 2023/10/04
CRDT- 2023/10/03 23:41
PHST- 2022/12/12 00:00 [received]
PHST- 2023/09/26 00:00 [accepted]
PHST- 2023/11/13 06:42 [medline]
PHST- 2023/10/04 00:42 [pubmed]
PHST- 2023/10/03 23:41 [entrez]
PHST- 2023/10/04 00:00 [pmc-release]
AID - 10.1007/s00441-023-03832-z [pii]
AID - 3832 [pii]
AID - 10.1007/s00441-023-03832-z [doi]
PST - ppublish
SO  - Cell Tissue Res. 2023 Nov;394(2):361-377. doi: 10.1007/s00441-023-03832-z. Epub 
      2023 Oct 4.