PMID- 37789395
OWN - NLM
STAT- MEDLINE
DCOM- 20231005
LR  - 20231121
IS  - 1477-3155 (Electronic)
IS  - 1477-3155 (Linking)
VI  - 21
IP  - 1
DP  - 2023 Oct 3
TI  - Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells 
      counteract oxidative damage and facilitate tendon regeneration.
PG  - 359
LID - 10.1186/s12951-023-02125-5 [doi]
LID - 359
AB  - BACKGROUND: Tendon injuries have a high incidence and limited treatment options. 
      Stem cell transplantation is essential for several medical conditions like tendon 
      injuries. However, high local concentrations of reactive oxygen species (ROS) 
      inhibit the activity of transplanted stem cells and hinder tendon repair. Cerium 
      oxide nanoparticles (CeONPs) have emerged as antioxidant agents with reproducible 
      reducibility. RESULTS: In this study, we synthesized polyethylene glycol-packed 
      CeONPs (PEG-CeONPs), which were loaded into the human umbilical cord mesenchymal 
      stem cells (hUCMSCs) to counteract oxidative damage. H(2)O(2) treatment was 
      performed to evaluate the ROS scavenging ability of PEG-CeONPs in hUCMSCs. A rat 
      model of patellar tendon defect was established to assess the effect of 
      PEG-CeONPs-carrying hUCMSCs in vivo. The results showed that PEG-CeONPs exhibited 
      excellent antioxidant activity both inside and outside the hUCMSCs. PEG-CeONPs 
      protect hUCMSCs from senescence and apoptosis under excessive oxidative stress. 
      Transplantation of hUCMSCs loaded with PEG-CeONPs reduced ROS levels in the 
      tendon injury area and facilitated tendon healing. Mechanistically, NFkappaB 
      activator tumor necrosis factor alpha and MAPK activator dehydrocrenatine, reversed 
      the therapeutic effect of PEG-CeONPs in hUCMSCs, indicating that PEG-CeONPs act 
      by inhibiting the NFkappaB and MAPK signaling pathways. CONCLUSIONS: The carriage of 
      the metal antioxidant oxidase PEG-CeONPs maintained the ability of hUCMSCs in the 
      injured area, reduced the ROS levels in the microenvironment, and facilitated 
      tendon regeneration. The data presented herein provide a novel therapeutic 
      strategy for tendon healing and new insights into the use of stem cells for 
      disease treatment.
CI  - (c) 2023. BioMed Central Ltd., part of Springer Nature.
FAU - Ren, Xunshan
AU  - Ren X
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.
FAU - Zhuang, Huangming
AU  - Zhuang H
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.
FAU - Zhang, Yuelong
AU  - Zhang Y
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.
FAU - Zhou, Panghu
AU  - Zhou P
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China. 
      zhoupanghu@whu.edu.cn.
LA  - eng
GR  - 2021BCA147/the Hubei Provincial key research and development program/
GR  - JCRCFZ-2022-019/Cross-Innovation Talent Program of Renmin Hospital of Wuhan 
      University/
GR  - 2042023kf0224/Fundamental Research Funds for Central Universities of the Central 
      South University/
PT  - Journal Article
DEP - 20231003
PL  - England
TA  - J Nanobiotechnology
JT  - Journal of nanobiotechnology
JID - 101152208
RN  - 619G5K328Y (ceric oxide)
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Reactive Oxygen Species
MH  - Hydrogen Peroxide
MH  - Oxidative Stress
MH  - *Mesenchymal Stem Cells
MH  - Regeneration
MH  - *Nanoparticles/therapeutic use
MH  - Tendons
MH  - *Tendon Injuries/therapy
MH  - Umbilical Cord
PMC - PMC10546722
OTO - NOTNLM
OT  - Cerium oxide
OT  - Human umbilical cord mesenchymal stem cell
OT  - Reactive oxygen species
OT  - Tendon
COIS- The authors declare no competing interests.
EDAT- 2023/10/04 00:42
MHDA- 2023/10/05 06:43
PMCR- 2023/10/03
CRDT- 2023/10/03 23:52
PHST- 2023/06/27 00:00 [received]
PHST- 2023/09/21 00:00 [accepted]
PHST- 2023/10/05 06:43 [medline]
PHST- 2023/10/04 00:42 [pubmed]
PHST- 2023/10/03 23:52 [entrez]
PHST- 2023/10/03 00:00 [pmc-release]
AID - 10.1186/s12951-023-02125-5 [pii]
AID - 2125 [pii]
AID - 10.1186/s12951-023-02125-5 [doi]
PST - epublish
SO  - J Nanobiotechnology. 2023 Oct 3;21(1):359. doi: 10.1186/s12951-023-02125-5.