PMID- 37789826
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231024
IS  - 1554-8937 (Electronic)
IS  - 1554-8929 (Linking)
VI  - 18
IP  - 10
DP  - 2023 Oct 20
TI  - Illuminating miRNA Inhibition: Visualizing the Interaction between Anti-miRNA 
      Oligonucleotide and Target miRNA Using FRET.
PG  - 2281-2289
LID - 10.1021/acschembio.3c00353 [doi]
AB  - Anti-miRNA oligonucleotides (anti-miRs) effectively and specifically inhibit the 
      function of individual miRNAs and have the potential to serve as a novel class of 
      nucleic acid therapeutic. However, the details of the mechanisms of anti-miRs in 
      cells have not yet been clarified sufficiently. In particular, the localization 
      of the complexes of anti-miRs and target miRNA in cells remains unclear. We 
      previously developed anti-miRs composed of serinol nucleic acid (SNA) that very 
      effectively inhibited miRNA-mediated silencing activity. Here we describe an 
      imaging system based on the fluorescence resonance energy transfer (FRET) 
      designed by miRNAs labeled with fluorophore-quencher pairs and an SNA-based 
      anti-miR labeled with an acceptor dye. We discovered that the anti-miR hybridizes 
      with the miRNA in the miRNA-induced silencing complex (miRISC), which is the 
      active complex formed by miRNA and Ago2 in cells within P-bodies. Based on FRET 
      ratio analysis, we hypothesize that the complex formed by the anti-miR and the 
      miRNA in P-bodies is dynamic, with anti-miR complexing the miRISC, followed by 
      miRNA release and degradation. Our findings provide valuable insights into the 
      mechanism of action of anti-miRs and enable further studies of miRNA-targeted 
      therapeutics.
FAU - Zhu, Hongyu
AU  - Zhu H
AUID- ORCID: 0009-0009-4226-1819
AD  - Department of Bimolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
FAU - Kamiya, Yukiko
AU  - Kamiya Y
AUID- ORCID: 0000-0001-7582-3886
AD  - Department of Bimolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
FAU - Asanuma, Hiroyuki
AU  - Asanuma H
AUID- ORCID: 0000-0001-9903-7847
AD  - Department of Bimolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231004
PL  - United States
TA  - ACS Chem Biol
JT  - ACS chemical biology
JID - 101282906
RN  - 0 (MicroRNAs)
RN  - 0 (Oligonucleotides)
RN  - 0 (Antagomirs)
SB  - IM
MH  - *MicroRNAs/metabolism
MH  - Oligonucleotides
MH  - Fluorescence Resonance Energy Transfer
MH  - Antagomirs
EDAT- 2023/10/04 06:43
MHDA- 2023/10/23 01:18
CRDT- 2023/10/04 03:53
PHST- 2023/10/23 01:18 [medline]
PHST- 2023/10/04 06:43 [pubmed]
PHST- 2023/10/04 03:53 [entrez]
AID - 10.1021/acschembio.3c00353 [doi]
PST - ppublish
SO  - ACS Chem Biol. 2023 Oct 20;18(10):2281-2289. doi: 10.1021/acschembio.3c00353. 
      Epub 2023 Oct 4.