PMID- 37790436
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240210
DP  - 2023 Sep 22
TI  - 5'UTR G-quadruplex structure enhances translation in size dependent manner.
LID - rs.3.rs-3352233 [pii]
LID - 10.21203/rs.3.rs-3352233/v1 [doi]
AB  - Translation initiation in bacteria is frequently regulated by various structures 
      in the 5' untranslated region (5'UTR). Previously, we demonstrated that 
      G-quadruplex (G4) formation in non-template DNA enhances transcription. In this 
      study, we aimed to explore how G4 formation in mRNA (RG4) at 5'UTR impacts 
      translation using a T7-based in vitro translation system and in E. coli. We 
      showed that RG4 strongly promotes translation efficiency in a size-dependent 
      manner. Additionally, inserting a hairpin upstream of the RG4 further enhances 
      translation efficiency, reaching up to a 12-fold increase. We found that the 
      RG4-dependent effect is not due to increased ribosome affinity, ribosome binding 
      site accessibility, or mRNA stability. We proposed a physical barrier model in 
      which bulky structures in 5'UTR prevent ribosome dislodging and thereby increase 
      the translation output. This study provides biophysical insights into the 
      regulatory role of 5'UTR structures in bacterial translation, highlighting their 
      potential applications in tuning gene expression.
FAU - Myong, Sua
AU  - Myong S
AUID- ORCID: 0000-0001-9098-3423
AD  - Boston Children's Hospital/Harvard Medical School.
FAU - Lee, Chun-Ying
AU  - Lee CY
AD  - Boston Children's Hospital/Harvard Medical School.
FAU - Joshi, Meera
AU  - Joshi M
AD  - Boston Children's Hospital/Harvard Medical School.
FAU - Wang, Ashley
AU  - Wang A
AUID- ORCID: 0000-0001-6542-999X
AD  - Johns Hopkins University.
LA  - eng
GR  - R01 GM149729/GM/NIGMS NIH HHS/United States
PT  - Preprint
DEP - 20230922
PL  - United States
TA  - Res Sq
JT  - Research square
JID - 101768035
PMC - PMC10543253
COIS- Declarations Competing interests The authors declare no competing interests.
EDAT- 2023/10/04 06:44
MHDA- 2023/10/04 06:45
PMCR- 2023/10/02
CRDT- 2023/10/04 04:04
PHST- 2023/10/04 06:44 [pubmed]
PHST- 2023/10/04 06:45 [medline]
PHST- 2023/10/04 04:04 [entrez]
PHST- 2023/10/02 00:00 [pmc-release]
AID - rs.3.rs-3352233 [pii]
AID - 10.21203/rs.3.rs-3352233/v1 [doi]
PST - epublish
SO  - Res Sq [Preprint]. 2023 Sep 22:rs.3.rs-3352233. doi: 10.21203/rs.3.rs-3352233/v1.