PMID- 37791056
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231005
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 18
IP  - 1
DP  - 2023
TI  - Bacterial lipoprotein plays an important role in the macrophage autophagy and 
      apoptosis induced by Salmonella typhimurium and Staphylococcus aureus.
PG  - 20220739
LID - 10.1515/biol-2022-0739 [doi]
LID - 20220739
AB  - This study aimed to determine the role of bacterial lipoprotein (BLP) in 
      autophagy and apoptosis. Western blot was used to examine autophagy biomarkers in 
      mouse bone marrow-derived macrophages (BMDMs) after infection with Salmonella 
      typhimurium (S. typhimurium) and Staphylococcus aureus (S. aureus) and BLP 
      stimulation. In BMDMs, enhanced protein expression of LC3-II was observed after 
      S. typhimurium or S. aureus infection (P < 0.05) and BLP stimulation (P < 0.05). 
      Autophagy inhibition by chloroquine resulted in increased levels of LC3-Ⅱ and p62 
      protein (P < 0.05). Persistently upregulated expressions of Atg3 and Atg7 were 
      observed following BLP stimulation (P < 0.05), and knockdown of Atg3 or Atg7 
      significantly attenuated BLP-enhanced protein expression of LC3-Ⅱ in BMDMs. 
      Furthermore, we found that the autophagy inhibitor 3-methyladenine prevented BLP- 
      and infection-induced macrophage apoptosis. BLP is not only required for 
      autophagy and apoptosis activation in macrophages but also for regulating the 
      balance between autophagy and apoptosis.
CI  - (c) 2023 the author(s), published by De Gruyter.
FAU - Jiang, Shanshan
AU  - Jiang S
AD  - Institute of Hematological Research, Shaanxi Provincial People's Hospital, Xi'an, 
      Shaanxi, 71000, China.
FAU - He, Jinyao
AU  - He J
AD  - Clinical Laboratory, Xi'an Medical University, Xi'an, Shaanxi, 710068, China.
FAU - Zhang, Lijie
AU  - Zhang L
AD  - Institute of Hematological Research, Shaanxi Provincial People's Hospital, Xi'an, 
      Shaanxi, 71000, China.
FAU - Zhao, Qiaojiajie
AU  - Zhao Q
AD  - Institute of Hematological Research, Shaanxi Provincial People's Hospital, Xi'an, 
      Shaanxi, 71000, China.
FAU - Zhao, Shuqi
AU  - Zhao S
AD  - Institute of Hematological Research, Shaanxi Provincial People's Hospital, Xi'an, 
      Shaanxi, 71000, China.
LA  - eng
PT  - Journal Article
DEP - 20230930
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC10543702
OTO - NOTNLM
OT  - apoptosis
OT  - autophagy
OT  - bacterial infection
OT  - bacterial lipoprotein
OT  - bone marrow-derived macrophages
COIS- Conflict of interest: Authors state no conflict of interest.
EDAT- 2023/10/04 06:43
MHDA- 2023/10/04 06:44
PMCR- 2023/09/30
CRDT- 2023/10/04 04:16
PHST- 2023/06/02 00:00 [received]
PHST- 2023/08/04 00:00 [revised]
PHST- 2023/09/04 00:00 [accepted]
PHST- 2023/10/04 06:44 [medline]
PHST- 2023/10/04 06:43 [pubmed]
PHST- 2023/10/04 04:16 [entrez]
PHST- 2023/09/30 00:00 [pmc-release]
AID - biol-2022-0739 [pii]
AID - 10.1515/biol-2022-0739 [doi]
PST - epublish
SO  - Open Life Sci. 2023 Sep 30;18(1):20220739. doi: 10.1515/biol-2022-0739. 
      eCollection 2023.