PMID- 37793240 OWN - NLM STAT- MEDLINE DCOM- 20231030 LR - 20231031 IS - 2213-2317 (Electronic) IS - 2213-2317 (Linking) VI - 67 DP - 2023 Nov TI - Associations of essential trace elements with epigenetic aging indicators and the potential mediating role of inflammation. PG - 102910 LID - S2213-2317(23)00311-7 [pii] LID - 10.1016/j.redox.2023.102910 [doi] LID - 102910 AB - BACKGROUND: Essential trace elements (ETEs) play essential roles in vital functions, but their effects on epigenetic aging remain poorly understood. OBJECTIVES: This study aimed to investigate the associations of ETEs with four epigenetic aging indicators and assess the potential mediating role of inflammation. METHODS: We recruited 93 individuals from hospitals between October 2018 and August 2019. Plasma levels of cobalt, copper, iron, manganese, molybdenum, selenium, and zinc were measured by ICP-MS, and leukocyte DNA methylation levels were measured using Illumina MethylationEPIC beadchip. Linear regression was used to estimate the association between seven plasma ETEs and epigenetic aging indicators. Weighted quantile sum (WQS) regression and Bayesian kernel machine regression (BKMR) models were used to evaluate the effect of ETEs mixtures. Inflammatory status was assessed using four systemic inflammation indices (neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), and systemic immune-inflammation index (SII)) and three cytokines (IL-4, IL-6, and IL-13). Mediation analysis was performed to explore the role of inflammation in the above associations. RESULTS: Plasma Se levels were significantly negatively associated with DunedinPACE, whereas Cu levels were significantly positively associated with it. Both WQS regression and BKMR models suggested that Se and Cu dominate the effect of the ETEs mixture. MLR and interleukin 6 were significantly and positively associated with DunedinPACE. Further mediation analysis indicated that inflammation partially mediated the association between ETEs and DunedinPACE. DISCUSSION: Plasma Se and Cu levels are closely associated to epigenetic aging, and inflammation might be a potential mechanism underlying this relationship. These findings contribute to the prevention of health hazards associated with population aging. CI - Copyright (c) 2023 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Cheng, Xu AU - Cheng X AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - Wei, Yue AU - Wei Y AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - Wang, Ruixin AU - Wang R AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - Jia, Chengyong AU - Jia C AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - Zhang, Zefang AU - Zhang Z AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - An, Jun AU - An J AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - Li, Weiya AU - Li W AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - Zhang, Jiazhen AU - Zhang J AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. FAU - He, Meian AU - He M AD - Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address: hemeian@hotmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230929 PL - Netherlands TA - Redox Biol JT - Redox biology JID - 101605639 RN - 0 (Trace Elements) RN - 789U1901C5 (Copper) SB - IM MH - Humans MH - *Trace Elements/metabolism MH - Copper MH - Bayes Theorem MH - Inflammation/genetics MH - Aging/genetics MH - Epigenesis, Genetic PMC - PMC10562911 OTO - NOTNLM OT - Copper OT - Epigenetic aging OT - Essential trace element OT - Inflammation OT - Selenium COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/10/05 01:00 MHDA- 2023/10/30 06:47 PMCR- 2023/09/29 CRDT- 2023/10/04 18:00 PHST- 2023/09/01 00:00 [received] PHST- 2023/09/27 00:00 [revised] PHST- 2023/09/27 00:00 [accepted] PHST- 2023/10/30 06:47 [medline] PHST- 2023/10/05 01:00 [pubmed] PHST- 2023/10/04 18:00 [entrez] PHST- 2023/09/29 00:00 [pmc-release] AID - S2213-2317(23)00311-7 [pii] AID - 102910 [pii] AID - 10.1016/j.redox.2023.102910 [doi] PST - ppublish SO - Redox Biol. 2023 Nov;67:102910. doi: 10.1016/j.redox.2023.102910. Epub 2023 Sep 29.