PMID- 37794520
OWN - NLM
STAT- MEDLINE
DCOM- 20231006
LR  - 20231122
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 14
IP  - 1
DP  - 2023 Oct 4
TI  - Efficient expansion and delayed senescence of hUC-MSCs by microcarrier-bioreactor 
      system.
PG  - 284
LID - 10.1186/s13287-023-03514-1 [doi]
LID - 284
AB  - BACKGROUND: Human umbilical cord mesenchymal stem cells (hUC-MSCs) are widely 
      used in cell therapy due to their robust immunomodulatory and tissue regenerative 
      capabilities. Currently, the predominant method for obtaining hUC-MSCs for 
      clinical use is through planar culture expansion, which presents several 
      limitations. Specifically, continuous cell passaging can lead to cellular aging, 
      susceptibility to contamination, and an absence of process monitoring and 
      control, among other limitations. To overcome these challenges, the technology of 
      microcarrier-bioreactor culture was developed with the aim of ensuring the 
      therapeutic efficacy of cells while enabling large-scale expansion to meet 
      clinical requirements. However, there is still a knowledge gap regarding the 
      comparison of biological differences in cells obtained through different culture 
      methods. METHODS: We developed a culture process for hUC-MSCs using self-made 
      microcarrier and stirred bioreactor. This study systematically compares the 
      biological properties of hUC-MSCs amplified through planar culture and 
      microcarrier-bioreactor systems. Additionally, RNA-seq was employed to compare 
      the differences in gene expression profiles between the two cultures, 
      facilitating the identification of pathways and genes associated with cell aging. 
      RESULTS: The findings revealed that hUC-MSCs expanded on microcarriers exhibited 
      a lower degree of cellular aging compared to those expanded through planar 
      culture. Additionally, these microcarrier-expanded hUC-MSCs showed an enhanced 
      proliferation capacity and a reduced number of cells in the cell cycle 
      retardation period. Moreover, bioreactor-cultured cells differ significantly from 
      planar cultures in the expression of genes associated with the cytoskeleton and 
      extracellular matrix. CONCLUSIONS: The results of this study demonstrate that our 
      microcarrier-bioreactor culture method enhances the proliferation efficiency of 
      hUC-MSCs. Moreover, this culture method exhibits the potential to delay the 
      process of cell aging while preserving the essential stem cell properties of 
      hUC-MSCs.
CI  - (c) 2023. BioMed Central Ltd., part of Springer Nature.
FAU - Wang, Xia
AU  - Wang X
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai, 200237, People's Republic of China.
FAU - Ouyang, Liming
AU  - Ouyang L
AUID- ORCID: 0000-0003-3507-7895
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai, 200237, People's Republic of China. 
      ouyanglm@ecust.edu.cn.
FAU - Chen, Wenxia
AU  - Chen W
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai, 200237, People's Republic of China.
FAU - Cao, Yulin
AU  - Cao Y
AD  - Beijing Tang Yi Hui Kang Biomedical Technology Co., LTD, Beijing, 100032, 
      People's Republic of China.
FAU - Zhang, Lixin
AU  - Zhang L
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai, 200237, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231004
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
SB  - IM
MH  - Humans
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Cells, Cultured
MH  - Cellular Senescence
MH  - Stem Cells
MH  - Bioreactors
MH  - Umbilical Cord
MH  - Cell Differentiation
MH  - *Mesenchymal Stem Cell Transplantation
PMC - PMC10552362
OTO - NOTNLM
OT  - Bioreactor
OT  - Cell culture
OT  - Cell senescence
OT  - Human umbilical cord mesenchymal stem cells
OT  - Microcarrier
OT  - Transcriptome
COIS- Corresponding author Liming Ouyang has received research grants from Beijing Tang 
      Yi Hui Kang Biomedical Technology Co., Ltd. Author Yulin Cao is a senior 
      executive at Beijing Tang Yi Hui Kang Biomedical Technology Co., Ltd., that has 
      invested in the research and holds shares in the company. The other authors 
      declare no competing interests.
EDAT- 2023/10/05 01:00
MHDA- 2023/10/06 06:43
PMCR- 2023/10/04
CRDT- 2023/10/04 23:50
PHST- 2023/06/06 00:00 [received]
PHST- 2023/09/25 00:00 [accepted]
PHST- 2023/10/06 06:43 [medline]
PHST- 2023/10/05 01:00 [pubmed]
PHST- 2023/10/04 23:50 [entrez]
PHST- 2023/10/04 00:00 [pmc-release]
AID - 10.1186/s13287-023-03514-1 [pii]
AID - 3514 [pii]
AID - 10.1186/s13287-023-03514-1 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2023 Oct 4;14(1):284. doi: 10.1186/s13287-023-03514-1.