PMID- 37795951
OWN - NLM
STAT- MEDLINE
DCOM- 20231101
LR  - 20231101
IS  - 1365-294X (Electronic)
IS  - 0962-1083 (Linking)
VI  - 32
IP  - 21
DP  - 2023 Nov
TI  - Population genomic analyses reveal population structure and major hubs of 
      invasive Anopheles stephensi in the Horn of Africa.
PG  - 5695-5708
LID - 10.1111/mec.17136 [doi]
AB  - Anopheles stephensi invasion in the Horn of Africa (HoA) poses a substantial risk 
      of increased malaria disease burden in the region. An understanding of the 
      history of introduction(s), establishment(s) and potential A. stephensi sources 
      in the HoA is needed to predict future expansions and establish where they may be 
      effectively controlled. To this end, we take a landscape genomic approach to 
      assess A. stephensi origins and spread throughout the HoA, information essential 
      for vector control. Specifically, we assayed 2070 genome-wide single nucleotide 
      polymorphisms across 214 samples spanning 13 populations of A. stephensi from 
      Ethiopia and Somaliland collected in 2018 and 2020, respectively. Principal 
      component and genetic ancestry analyses revealed clustering that followed an 
      isolation-by-distance pattern, with genetic divergence among the Ethiopian 
      samples significantly correlating with geographical distance. Additionally, 
      genetic relatedness was observed between the northeastern and east central 
      Ethiopian A. stephensi populations and the Somaliland A. stephensi populations. 
      These results reveal population differentiation and genetic connectivity within 
      HoA A. stephensi populations. Furthermore, based on genetic network analysis, we 
      uncovered that Dire Dawa, the site of a spring 2022 malaria outbreak, was one of 
      the major hubs from which sequential founder events occurred in the rest of the 
      eastern Ethiopian region. These findings can be useful for the selection of sites 
      for heightened control to prevent future malaria outbreaks. Finally, we did not 
      detect significant genotype-environmental associations, potentially due to the 
      recency of their colonization and/or other anthropogenic factors leading to the 
      initial spread and establishment of A. stephensi. Our study highlights how 
      coupling genomic data at landscape levels can shed light into even ongoing 
      invasions.
CI  - (c) 2023 The Authors. Molecular Ecology published by John Wiley & Sons Ltd.
FAU - Samake, Jeanne N
AU  - Samake JN
AUID- ORCID: 0000-0003-2391-6583
AD  - Department of Biology, Baylor University, Waco, Texas, USA.
FAU - Lavretsky, Philip
AU  - Lavretsky P
AUID- ORCID: 0000-0002-5904-8821
AD  - Department of Biological Sciences, University of Texas at El Paso, El Paso, 
      Texas, USA.
FAU - Gunarathna, Isuru
AU  - Gunarathna I
AUID- ORCID: 0000-0001-9729-2879
AD  - Department of Biology, Baylor University, Waco, Texas, USA.
FAU - Follis, Madison
AU  - Follis M
AUID- ORCID: 0009-0000-6676-9830
AD  - Department of Biology, Baylor University, Waco, Texas, USA.
FAU - Brown, Joshua I
AU  - Brown JI
AUID- ORCID: 0000-0001-6299-9815
AD  - Department of Life, Earth, and Environmental Sciences, West Texas A&M University, 
      Canyon, Texas, USA.
FAU - Ali, Said
AU  - Ali S
AD  - Ministry of Health Somaliland, Hargeisa, Somalia.
FAU - Yared, Solomon
AU  - Yared S
AD  - Department of Biology, Jigjiga University, Jigjiga, Ethiopia.
FAU - Carter, Tamar E
AU  - Carter TE
AUID- ORCID: 0000-0002-9438-9541
AD  - Department of Biology, Baylor University, Waco, Texas, USA.
LA  - eng
GR  - Research Enhancement Award (R15AI151766)/NH/NIH HHS/United States
GR  - Research Enhancement Award (R15AI151766)/NH/NIH HHS/United States
PT  - Journal Article
DEP - 20231005
PL  - England
TA  - Mol Ecol
JT  - Molecular ecology
JID - 9214478
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Anopheles/genetics
MH  - Mosquito Vectors/genetics
MH  - Gene Regulatory Networks
MH  - Metagenomics
MH  - *Malaria/epidemiology/genetics
MH  - Genomics
MH  - Ethiopia
OTO - NOTNLM
OT  - ddRAD-seq
OT  - invasive mosquito
OT  - landscape genomics
OT  - malaria
EDAT- 2023/10/05 12:42
MHDA- 2023/11/01 12:44
CRDT- 2023/10/05 08:43
PHST- 2023/08/15 00:00 [revised]
PHST- 2022/11/29 00:00 [received]
PHST- 2023/09/04 00:00 [accepted]
PHST- 2023/11/01 12:44 [medline]
PHST- 2023/10/05 12:42 [pubmed]
PHST- 2023/10/05 08:43 [entrez]
AID - 10.1111/mec.17136 [doi]
PST - ppublish
SO  - Mol Ecol. 2023 Nov;32(21):5695-5708. doi: 10.1111/mec.17136. Epub 2023 Oct 5.