PMID- 37801891
OWN - NLM
STAT- MEDLINE
DCOM- 20231206
LR  - 20231228
IS  - 1872-7603 (Electronic)
IS  - 0165-0378 (Linking)
VI  - 160
DP  - 2023 Dec
TI  - Granulocyte-macrophage colony-stimulating factor promotes endometrial repair 
      after injury by regulating macrophages in mice.
PG  - 104156
LID - S0165-0378(23)00362-5 [pii]
LID - 10.1016/j.jri.2023.104156 [doi]
AB  - Intrauterine adhesion (IUA) caused by endometrial injury is a common cause of 
      female infertility and is challenging to treat. Macrophages play a critical role 
      in tissue repair and cyclical endometrial regeneration. Granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) has significant reparative and anti-fibrotic 
      effects in various tissues. However, there is limited research on the role of 
      GM-CSF in the repair of endometrial injury and the involvement of macrophages in 
      GM-CSF-mediated endometrial repair. In this study, using a mouse model of 
      endometrial scratching injury, we found that GM-CSF treatment accelerated the 
      repair of endometrial injury and improved fertility. At the molecular level, we 
      observed that GM-CSF can downregulate the transcript levels of tumor necrosis 
      factor (TNF) in mouse bone marrow-derived macrophages (BMDMs) stimulated by 
      lipopolysaccharide (LPS) and upregulate the expression of Arginase-1 (Arg-1) and 
      mannose receptor C-type 1 (MRC1). Importantly, during the early and middle stages 
      of injury, GM-CSF increased the proportion of M1-like, M2-like, and M1/M2 mixed 
      macrophages, while in the late stage of injury, GM-CSF facilitated a decline in 
      the number of M2-like macrophages. These findings suggest that GM-CSF may promote 
      endometrial repair by recruiting macrophages and modulating the LPS-induced 
      M1-like macrophages into a less inflammatory phenotype. These insights have the 
      potential to contribute to the development of novel therapeutic approaches for 
      the treatment of intrauterine adhesion and related infertility.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Zhu, Xiaohong
AU  - Zhu X
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China; Department of Obstetrics and 
      Gynecology, Affiliated Xiaoshan Hospital, Hangzhou Normal University (Zhejiang 
      Xiaoshan Hospital), 311201 Hangzhou, China.
FAU - Chen, Sijia
AU  - Chen S
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China.
FAU - Zhang, Peipei
AU  - Zhang P
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China; Department of Obstetrics and 
      Gynecology, Tiantai People's Hospital of Zhejiang Province, 317200 Taizhou, 
      China.
FAU - Ma, Yana
AU  - Ma Y
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China.
FAU - Liu, Xiu
AU  - Liu X
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China; Key Laboratory of Reproductive 
      Dysfunction, Management of Zhejiang Province, 310016 Hangzhou, China.
FAU - Fei, Haiyi
AU  - Fei H
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China; Key Laboratory of Reproductive 
      Dysfunction, Management of Zhejiang Province, 310016 Hangzhou, China.
FAU - Qian, Jingjing
AU  - Qian J
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China; Department of Obstetrics and 
      Gynecology, Yuyao People's Hospital, 315400 Ningbo, China.
FAU - Hao, Yanqing
AU  - Hao Y
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China.
FAU - Jiang, Lingling
AU  - Jiang L
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China; Key Laboratory of Reproductive 
      Dysfunction, Management of Zhejiang Province, 310016 Hangzhou, China. Electronic 
      address: linglingjiang@zju.edu.cn.
FAU - Lin, Xiaona
AU  - Lin X
AD  - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run 
      Shaw Hospital, Zhejiang University School of Medicine, No. 3 Qingchun East Road, 
      Jianggan District, Hangzhou 310016, China; Key Laboratory of Reproductive 
      Dysfunction, Management of Zhejiang Province, 310016 Hangzhou, China. Electronic 
      address: linna73@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230923
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Female
MH  - *Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - *Macrophages/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - *Endometrium/injuries
MH  - Animals
MH  - Mice
OTO - NOTNLM
OT  - Endometrial injury
OT  - Fibrosis
OT  - GM-CSF
OT  - Intrauterine adhesion
OT  - Macrophages
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2023/10/07 00:41
MHDA- 2023/12/05 12:42
CRDT- 2023/10/06 18:07
PHST- 2022/11/29 00:00 [received]
PHST- 2023/07/07 00:00 [revised]
PHST- 2023/09/20 00:00 [accepted]
PHST- 2023/12/05 12:42 [medline]
PHST- 2023/10/07 00:41 [pubmed]
PHST- 2023/10/06 18:07 [entrez]
AID - S0165-0378(23)00362-5 [pii]
AID - 10.1016/j.jri.2023.104156 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2023 Dec;160:104156. doi: 10.1016/j.jri.2023.104156. Epub 2023 
      Sep 23.