PMID- 37802477 OWN - NLM STAT- MEDLINE DCOM- 20231102 LR - 20231102 IS - 1879-1298 (Electronic) IS - 0045-6535 (Linking) VI - 344 DP - 2023 Dec TI - Gestational exposure to ambient fine particulate matter disrupts maternal hepatic lipid metabolism. PG - 140369 LID - S0045-6535(23)02639-5 [pii] LID - 10.1016/j.chemosphere.2023.140369 [doi] AB - Pregnancy is a unique physiological stage for females as well as a vulnerable period for pollutant exposure. The effect of gestational ambient fine particulate matter (PM(2.5)) exposure on maternal lipid metabolism during pregnancy is rarely observed, and the mechanism is unknown. In the current study, pregnant C57BL/6 mice were randomly assigned to either ambient PM(2.5) or filtered air exposure chambers since gestational day (GD) 0. Meanwhile, non-pregnant female mice were housed as controls in each exposure chamber. PM(2.5) exposure exerted no significant effect on body weight gain or the body composition during pregnancy. Pregnant mice exposed to PM(2.5) demonstrated improved glucose tolerance, whereas non-pregnant mice showed an increased fasting blood glucose level after PM(2.5) exposure with no alterations in glucose tolerance. PM(2.5) exposure exerted no significant effect on total lipid content in serum during pregnancy, while an increased serum total lipid level was found in non-pregnant mice exposed to PM(2.5). PM(2.5) exposure had no effect on total liver lipid levels, it increased several triacylglycerol (TAG) species and total cholesterol esters (CEs) in pregnant mice but lowered a considerable amount in non-pregnant mice' livers. Furthermore, gestational exposure to PM(2.5) enhanced the expression of key enzymes in fatty acid uptake, de novo lipid synthesis, and beta oxidation, and inhibited molecules for lipid export in mice liver. Conversely, PM(2.5) exposure upregulated proteins involved in hepatic lipolysis and lipid export in non-pregnant mice. These results suggest that the interference of PM(2.5) exposure during pregnancy on the lipid metabolism, particularly the hepatic lipid metabolism, differs from that during non-pregnancy. This study provides toxicological evidence that PM(2.5) exposure during pregnancy disrupts the lipid metabolism of the liver and provides a basis for protecting vulnerable populations. CI - Copyright (c) 2023 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Wu, Yunlu AU - Wu Y AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. FAU - Wang, Yirun AU - Wang Y AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. FAU - Zhang, Wenhui AU - Zhang W AD - Department of Environmental and Occupational Health, Hangzhou Center for Disease Control and Prevention, Hangzhou, Zhejiang, China. FAU - Peng, Jing AU - Peng J AD - Zhuantang Community Healthcare Center, Hangzhou, Zhejiang, China. FAU - Qin, Li AU - Qin L AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. FAU - Zhang, Lu AU - Zhang L AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. FAU - Chen, Rucheng AU - Chen R AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. FAU - Gu, Weijia AU - Gu W AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. FAU - Sun, Qinghua AU - Sun Q AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. FAU - Liu, Cuiqing AU - Liu C AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. Electronic address: liucuiqing@zcmu.edu.cn. FAU - Li, Ran AU - Li R AD - School of Public Health, Zhejiang International Science and Technology Cooperation Base of Air Pollution and Health, Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. Electronic address: ranli@zcmu.edu.cn. LA - eng PT - Journal Article DEP - 20231004 PL - England TA - Chemosphere JT - Chemosphere JID - 0320657 RN - 0 (Air Pollutants) RN - IY9XDZ35W2 (Glucose) RN - 0 (Lipids) RN - 0 (Particulate Matter) SB - IM MH - Animals MH - Female MH - Mice MH - Pregnancy MH - *Air Pollutants/toxicity MH - *Air Pollution MH - Glucose MH - Lipid Metabolism MH - Lipids MH - Liver MH - *Maternal Exposure/adverse effects MH - Mice, Inbred C57BL MH - Particulate Matter/analysis OTO - NOTNLM OT - Fine particulate matter OT - Gestational exposure OT - Lipid metabolism OT - Liver COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/10/07 00:42 MHDA- 2023/11/02 12:45 CRDT- 2023/10/06 19:31 PHST- 2023/07/02 00:00 [received] PHST- 2023/09/27 00:00 [revised] PHST- 2023/10/03 00:00 [accepted] PHST- 2023/11/02 12:45 [medline] PHST- 2023/10/07 00:42 [pubmed] PHST- 2023/10/06 19:31 [entrez] AID - S0045-6535(23)02639-5 [pii] AID - 10.1016/j.chemosphere.2023.140369 [doi] PST - ppublish SO - Chemosphere. 2023 Dec;344:140369. doi: 10.1016/j.chemosphere.2023.140369. Epub 2023 Oct 4.