PMID- 37802805
OWN - NLM
STAT- MEDLINE
DCOM- 20231101
LR  - 20231101
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 17
DP  - 2023 Sep
TI  - [Quality evaluation of Lysimachiae Herba from different habitats based on 
      simultaneous determination of multiple bioactive constituents combined with 
      multivariate statistical analysis].
PG  - 4663-4674
LID - 10.19540/j.cnki.cjcmm.20230519.101 [doi]
AB  - A method based on ultra-high performance liquid chromatography coupled with 
      triple quadrupole linear ion trap-tandem mass spectrometry(UHPLC-QTRAP-MS/MS) was 
      developed for the simultaneous determination of 41 bioactive constituents of 
      flavonoids, organic acids, nucleosides, and amino acids in Lysimachiae Herba. The 
      content of multiple bioactive constituents was compared among the samples from 
      different habitats. The chromatographic separation was performed in a Waters 
      XBridge(R)C_(18) column(4.6 mmx100 mm, 3.5 mum) at 30 ℃. The gradient elution was 
      performed with 0.4% methanol(A)-formic acid water(B) as the mobile phase at a 
      flow rate of 0.8 mL.min~(-1), and the multiple-reaction monitoring(MRM) mode was 
      adopted. According to the content of 41 constituents, hierarchical cluster 
      analysis(HCA), orthogonal partial least squares discriminant analysis(OPLS-DA), 
      and gray relational analysis(GRA) were perfomed to comprehensively evaluate the 
      samples from different habitats. The results showed that the 41 constituents 
      exhibited good linear relationship within the tested concentration ranges, with 
      the correlation coefficients(r) greater than 0.999 4. The method featured good 
      precision, repeatability, and stability with the relative standard 
      deviations(RSDs) less than 5.0%. The average recoveries of the 41 constituents 
      ranged from 98.06% to 101.9%, with the RSDs of 0.62%-4.6%. HCA and OPLS-DA 
      separated 48 batches of Lysimachiae Herba samples from different habitats into 
      three categories: the producing areas in Sichuan and Chongqing, the producing 
      areas in Jiangsu, Zhejiang, and Jiangxi, and the producing areas in Guizhou. The 
      content of 41 constituents varied among the Lysimachiae Herba samples from 
      different habitats. The GRA results revealed that the Lysimachiae Herba sample 
      from Nanchong City, Sichuan Province had the best comprehensive quality. The 
      method developed in this study was accurate and reliable and thus can be used for 
      comprehensive evaluation of Lysimachiae Herba quality and provide basic 
      information for the selection of habitats.
FAU - Zhou, Yong-Yi
AU  - Zhou YY
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Chen, Hai-Jie
AU  - Chen HJ
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Xue, Jia
AU  - Xue J
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Wu, Nan
AU  - Wu N
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Yuan, Jia-Huan
AU  - Yuan JH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Liu, Xun-Hong
AU  - Liu XH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China 
      Jiangsu Province Engineering Research Center of Classical Prescription Nanjing 
      210023, China.
FAU - Zou, Li-Si
AU  - Zou LS
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Chen, Cui-Hua
AU  - Chen CH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Cai, Zhi-Chen
AU  - Cai ZC
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Yang, Wei
AU  - Yang W
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Cheng, Jian-Ming
AU  - Cheng JM
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China 
      Jiangsu Province Engineering Research Center of Classical Prescription Nanjing 
      210023, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Amino Acids)
SB  - IM
MH  - *Tandem Mass Spectrometry/methods
MH  - Multivariate Analysis
MH  - Chromatography, High Pressure Liquid/methods
MH  - *Drugs, Chinese Herbal/chemistry
MH  - Amino Acids/analysis
OTO - NOTNLM
OT  - Lysimachiae Herba
OT  - UHPLC-QTRAP-MS/MS
OT  - different habitats
OT  - multiple bioactive constituents
OT  - multivariate statistical analysis
EDAT- 2023/10/07 00:41
MHDA- 2023/11/01 12:44
CRDT- 2023/10/06 22:03
PHST- 2023/11/01 12:44 [medline]
PHST- 2023/10/07 00:41 [pubmed]
PHST- 2023/10/06 22:03 [entrez]
AID - 10.19540/j.cnki.cjcmm.20230519.101 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Sep;48(17):4663-4674. doi: 
      10.19540/j.cnki.cjcmm.20230519.101.