PMID- 37802848
OWN - NLM
STAT- MEDLINE
DCOM- 20231009
LR  - 20231018
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 18
DP  - 2023 Sep
TI  - [Mechanism of Astragali Radix-Curcumae Rhizoma in treating gastric cancer based 
      on network pharmacology and experimental verification].
PG  - 5056-5067
LID - 10.19540/j.cnki.cjcmm.20230601.701 [doi]
AB  - This study aims to investigate the mechanism of Astragali Radix-Curcumae 
      Rhizoma(HQEZ) in the treatment of gastric cancer based on network pharmacology. 
      Further, the SGC7901 cell model of gastric cancer was employed to validate the 
      efficacy and key targets of the herb pair. Firstly, the CCK-8 assay was employed 
      to evaluate the direct effect of HQEZ on the proliferation of gastric cancer 
      SGC7901 cells. Then, network pharmacology methods were employed to investigate 
      the active ingredients, key targets, and key signaling pathways involved in the 
      treatment of gastric cancer with HQEZ. The results showed that HQEZ contained 18 
      potential active ingredients, such as quercetin, naringenin, and curcumin. The 
      results of gene ontology(GO) functional annotation and Kyoto Encyclopedia of 
      Genes and Genomes(KEGG) pathway enrichment suggested that the main targets of 
      HQEZ in treating gastric cancer were involved in the regulation of protein 
      serine/threonine kinase activity, activation of mitogen-activated protein 
      kinase(MAPK) activity, cysteine-type endopeptidase activity, and negative 
      regulation of protein serine/threonine kinase activity. The hypoxia-inducible 
      factor-1(HIF-1) signaling pathway, ATP-binding cassette(ABC) transporters, 
      cytochrome P450-mediated metabolism of xenobiotics, p53 signaling pathway, and 
      cell apoptosis were key signaling pathways of HQEZ in treating gastric cancer. 
      The cell experiments demonstrated that HQEZ significantly downregulated the 
      expression of ATP-binding cassette subfamily B member 1(ABCB1), epidermal growth 
      factor receptor(EGFR), phosphorylated serine/threonine kinase(p-AKT), hypoxia 
      inducible factor 1 subunit alpha(HIF1A), B-cell lymphoma 2(BCL2), breast cancer 
      susceptibility protein 1(BRCA1), DNA polymerase theta(POLH), ribonucleotide 
      reductase M1(RRM1), and excision repair cross-complementation group 1(ERCC1), and 
      upregulated the expression of tumor protein P53(TP53) and cysteinyl 
      aspartate-specific proteinase(CAPS3). Finally, a multivariate COX regression 
      model was adopted to study the relationship between gene expression and clinical 
      information data of gastric cancer patients in the TCGA database, which 
      demonstrated that the key targets of HQEZ were associated with the poor prognosis 
      in gastric cancer patients. Further feature selection using the LASSO algorithm 
      showed that EGFR, HIF1A, TP53, POLH, RRM1, and ERCC1 were closely associated with 
      the survival of gastric can-cer patients. In conclusion, HQEZ regulates the 
      expression of genes involved in DNA repair, survival, and apoptosis in gastric 
      cancer cells via multiple targets and pathways, assisting the treatment of 
      gastric cancer.
FAU - Tan, Xi-Ying
AU  - Tan XY
AD  - Department of Pharmacy, Affiliated Hospital of Nanjing University of Chinese 
      Medicine Nanjing 210029, China.
FAU - Tao, Jing
AU  - Tao J
AD  - China Pharmaceutical University Nanjing 210009, China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Nanjing Medical University Nanjing 211166, China.
FAU - Gu, Ru-Xin
AU  - Gu RX
AD  - Nanjing Medical University Nanjing 211166, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - 452VLY9402 (Serine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Humans
MH  - *Stomach Neoplasms/drug therapy/genetics
MH  - Tumor Suppressor Protein p53
MH  - Network Pharmacology
MH  - ErbB Receptors
MH  - Protein Serine-Threonine Kinases
MH  - Serine
MH  - Adenosine Triphosphate
MH  - Molecular Docking Simulation
MH  - *Drugs, Chinese Herbal/pharmacology
OTO - NOTNLM
OT  - Astragali Radix-Curcumae Rhizoma
OT  - gastric cancer
OT  - herb pair
OT  - mechanism of action
OT  - network pharmacology
EDAT- 2023/10/07 00:42
MHDA- 2023/10/09 06:41
CRDT- 2023/10/06 22:03
PHST- 2023/10/09 06:41 [medline]
PHST- 2023/10/07 00:42 [pubmed]
PHST- 2023/10/06 22:03 [entrez]
AID - 10.19540/j.cnki.cjcmm.20230601.701 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Sep;48(18):5056-5067. doi: 
      10.19540/j.cnki.cjcmm.20230601.701.