PMID- 37802874
OWN - NLM
STAT- MEDLINE
DCOM- 20231101
LR  - 20231101
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 16
DP  - 2023 Aug
TI  - [Morin induces autophagy and apoptosis in hepatocellular carcinoma cells through 
      Akt/mTOR/STAT3 pathway].
PG  - 4475-4482
LID - 10.19540/j.cnki.cjcmm.20230413.704 [doi]
AB  - This study investigated the effect and mechanism of morin in inducing autophagy 
      and apoptosis in hepatocellular carcinoma cells through the protein kinase 
      B(Akt)/mammalian target of rapamycin(mTOR)/signal transducer and activator of 
      transcription protein 3(STAT3) pathway. Human hepatocellular carcinoma SK-HEP-1 
      cells were stimulated with different concentrations of morin(0, 50, 100, 125, 
      200, and 250 mumol.L~(-1)). The effect of morin on the viability of SK-HEP-1 cells 
      was detected by Cell Counting Kit-8(CCK-8). The effect of morin on the 
      proliferation and apoptosis of SK-HEP-1 cells was investigated using colony 
      formation assay, flow cytometry, and BeyoClick~(TM) EdU-488 with different 
      concentrations of morin(0, 125, and 250 mumol.L~(-1)). The changes in the 
      autophagy level of cells treated with morin were examined by transmission 
      electron microscopy and autophagy inhibitors. The impact of morin on the 
      expression levels of proteins related to the Akt/mTOR/STAT3 pathway was verified 
      by Western blot. Compared with the control group, the morin groups showed 
      decreased viability of SK-HEP-1 cells in a time-and concentration-dependent 
      manner, increased number of apoptotic cells, up-regulated expression level of 
      apoptosis marker PARP, up-regulated phosphorylation level of apoptosis-regulating 
      protein H2AX, decreased number of positive cells and the colony formation rate, 
      an upward trend of expression levels of autophagy-related proteins LC3-Ⅱ, Atg5, 
      and Atg7, and decreased phosphorylation levels of Akt, mTOR, and STAT3. These 
      results suggest that morin can promote apoptosis, inhibit proliferation, and 
      induce autophagy in hepatocellular carcinoma cells, and its mechanism of action 
      may be related to the Akt/mTOR/STAT3 pathway.
FAU - Zhao, Xin-Yue
AU  - Zhao XY
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Tian, Ying-Ying
AU  - Tian YY
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Liu, Chuang
AU  - Liu C
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Li, Yi-Lin
AU  - Li YL
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Lyu, Ying-Nan
AU  - Lyu YN
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Yu, Shang-Yue
AU  - Yu SY
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Tian, Shi-Qiu
AU  - Tian SQ
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Pei, Hai-Luan
AU  - Pei HL
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China.
FAU - Zuo, Ze-Ping
AU  - Zuo ZP
AD  - Beijing Tongrentang Technologies Co.,Ltd.,Pharmaceutical Factory Beijing 
      100079,China.
FAU - Wang, Zhi-Bin
AU  - Wang ZB
AD  - School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 
      102488,China Beijing Tongrentang Technologies Co.,Ltd.,Pharmaceutical Factory 
      Beijing 100079,China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - 8NFQ3F76WR (morin)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (STAT3 protein, human)
RN  - 0 (STAT3 Transcription Factor)
SB  - IM
MH  - Humans
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - *Carcinoma, Hepatocellular/drug therapy/pathology
MH  - *Liver Neoplasms/drug therapy/pathology
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Apoptosis
MH  - Autophagy
MH  - Cell Proliferation
MH  - Cell Line, Tumor
MH  - STAT3 Transcription Factor/genetics/metabolism
OTO - NOTNLM
OT  - Akt/mTOR/STAT3 pathway
OT  - apoptosis
OT  - autophagy
OT  - hepatocellular carcinoma
OT  - morin
OT  - proliferation
EDAT- 2023/10/07 00:41
MHDA- 2023/11/01 12:42
CRDT- 2023/10/06 22:03
PHST- 2023/11/01 12:42 [medline]
PHST- 2023/10/07 00:41 [pubmed]
PHST- 2023/10/06 22:03 [entrez]
AID - 10.19540/j.cnki.cjcmm.20230413.704 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Aug;48(16):4475-4482. doi: 
      10.19540/j.cnki.cjcmm.20230413.704.