PMID- 37804896 OWN - NLM STAT- MEDLINE DCOM- 20231127 LR - 20231127 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 253 IP - Pt 5 DP - 2023 Dec 31 TI - Phenolated alginate hydrogel induced osteogenic properties of mesenchymal stem cells via Wnt signaling pathway. PG - 127209 LID - S0141-8130(23)04106-5 [pii] LID - 10.1016/j.ijbiomac.2023.127209 [doi] AB - Osteogenic properties of phenolated alginate (1.2 %) hydrogel containing collagen (0.5 %)/nano-hydroxyapatite (1 %) were studied on human mesenchymal stem cells in vitro. The phenolation rate and physical properties of the hydrogel were assessed using nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), swelling ratio, gelation time, mechanical assay, and degradation rate. The viability of encapsulated cells was monitored on days 7, 14, and 21 using an MTT assay. Osteoblast differentiation was studied using western blotting, and real-time PCR. Using PCR array analysis, the role of the Wnt signaling pathway was also investigated. Data showed that the combination of alginate/collagen/nanohydroxyapatite yielded proper mechanical features. The addition of nanohydroxyapatite, and collagen reduced degradation, swelling rate coincided with increased stiffness. Elasticity and pore size were also diminished. NMR and FTIR revealed suitable incorporation of collagen and nanohydroxyapatite in the structure of alginate. Real-time PCR analysis and western blotting indicated the expression of osteoblast-related genes such as Runx2 and osteocalcin. PCR array revealed the induction of numerous genes related to Wnt signaling pathways during the maturation of human stem cells toward osteoblast-like cells. In vivo data indicated that transplantation of phenolated alginate/collagen/nanohydroxyapatite hydrogel led to enhanced de novo bone formation in rats with critical-sized calvarial defects. Phenolated alginate hydrogel can promote the osteogenic capacity of human amniotic membrane mesenchymal stem cells in the presence of nanohydroxyapatite and collagen via engaging the Wnt signaling pathway. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - Saghati, Sepideh AU - Saghati S AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Avci, Cigir Biray AU - Avci CB AD - Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey. FAU - Hassani, Ayla AU - Hassani A AD - Chemical Engineering Faculty, Sahand University of Technology, Tabriz 51335-1996, Iran; Stem Cell and Tissue Engineering Research Laboratory, Sahand University of Technology, Tabriz 51335-1996, Iran. FAU - Nazifkerdar, Sajed AU - Nazifkerdar S AD - Chemical Engineering Faculty, Sahand University of Technology, Tabriz 51335-1996, Iran; Stem Cell and Tissue Engineering Research Laboratory, Sahand University of Technology, Tabriz 51335-1996, Iran. FAU - Amini, Hassan AU - Amini H AD - Department of General and Vascular Surgery, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Saghebasl, Solmaz AU - Saghebasl S AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Mahdipour, Mahdi AU - Mahdipour M AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Banimohamad-Shotorbani, Behnaz AU - Banimohamad-Shotorbani B AD - Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Namjoo, Atieh Rezaei AU - Namjoo AR AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Abrbekoh, Fateme Nazary AU - Abrbekoh FN AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Rahbarghazi, Reza AU - Rahbarghazi R AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: rahbarghazir@tbzmed.ac.ir. FAU - Nasrabadi, Hamid Tayefi AU - Nasrabadi HT AD - Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Tayefih@yahoo.com. FAU - Khoshfetrat, Ali Baradar AU - Khoshfetrat AB AD - Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey; Chemical Engineering Faculty, Sahand University of Technology, Tabriz 51335-1996, Iran. Electronic address: khoshfetrat@sut.ac.ir. LA - eng PT - Journal Article DEP - 20231005 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 0 (Hydrogels) RN - 0 (Alginates) RN - 9007-34-5 (Collagen) SB - IM MH - Humans MH - Rats MH - Animals MH - *Osteogenesis MH - Hydrogels/chemistry MH - Wnt Signaling Pathway MH - Alginates/chemistry MH - Collagen/metabolism MH - Cell Differentiation MH - *Mesenchymal Stem Cells MH - Cells, Cultured MH - Tissue Scaffolds/chemistry OTO - NOTNLM OT - Mesenchymal stem cells OT - Osteogenesis OT - Phenolated alginate/collagen/nanohydroxyapatite COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/10/08 02:42 MHDA- 2023/11/27 12:42 CRDT- 2023/10/07 19:24 PHST- 2022/08/20 00:00 [received] PHST- 2023/09/06 00:00 [revised] PHST- 2023/10/01 00:00 [accepted] PHST- 2023/11/27 12:42 [medline] PHST- 2023/10/08 02:42 [pubmed] PHST- 2023/10/07 19:24 [entrez] AID - S0141-8130(23)04106-5 [pii] AID - 10.1016/j.ijbiomac.2023.127209 [doi] PST - ppublish SO - Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127209. doi: 10.1016/j.ijbiomac.2023.127209. Epub 2023 Oct 5.