PMID- 37805391
OWN - NLM
STAT- MEDLINE
DCOM- 20231102
LR  - 20231102
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 52
IP  - 10
DP  - 2023 Oct 8
TI  - [Clinicopathological and prognostic features of subungual melanoma in situ].
PG  - 1006-1011
LID - 10.3760/cma.j.cn112151-20230226-00152 [doi]
AB  - Objective: To investigate the clinicopathological characteristics, 
      immunohistochemical profiles, molecular features, and prognosis of subungual 
      melanoma in situ (SMIS). Methods: Thirty cases of SMIS were collected in Fudan 
      University Shanghai Cancer Center, Shanghai, China from 2018 to 2022. The 
      clinicopathological characteristics and follow-up data were retrospectively 
      analyzed. Histopathologic evaluation and immunohistochemical studies were carried 
      out. By using Vysis melanoma fluorescence in situ hybridization (FISH) probe kit, 
      combined with 9p21(CDKN2A) and 8q24(MYC) assays were performed. Results: There 
      were 8 males and 22 females. The patients' ages ranged from 22 to 65 years 
      (median 48 years). All patients presented with longitudinal melanonychia 
      involving a single digit. Thumb was the most commonly affected digit (16/30, 
      53.3%). 56.7% (17/30) of the cases presented with Hutchinson's sign. 
      Microscopically, melanocytes proliferated along the dermo-epithelial junction. 
      Hyperchromatism and nuclear pleomorphism were two of the most common histological 
      features. The melanocyte count ranged from 30 to 185. Most cases showed small to 
      medium nuclear enlargement (29/30, 96.7%). Pagetoid spread was seen in all cases. 
      Intra-epithelial mitoses were identified in 56.7% (17/30) of the cases. 
      Involvement of nailfold was found in 19 cases, 4 of which were accompanied by 
      cutaneous adnexal extension. The positive rates of SOX10, PNL2, Melan A, HMB45, 
      S-100, and PRAME were 100.0%, 100.0%, 96.0%, 95.0%, 76.9%, and 83.3%, 
      respectively. FISH analysis was positive in 6/9 of the cases. Follow-up data were 
      available in 28 patients, and all of them were alive without disease. 
      Conclusions: SMIS mainly shows small to medium-sized cells. High melanocyte 
      count, hyperchromatism, nuclear pleomorphism, Pagetoid spreading, 
      intra-epithelial mitosis, nailfold involvement, and cutaneous adnexal extension 
      are important diagnostic hallmarks. Immunohistochemistry including SOX10 and 
      PRAME, combined with FISH analysis, is valuable for the diagnosis of SMIS.
FAU - Hu, J
AU  - Hu J
AD  - Department of Pathology, Fudan University Shanghai Cancer Center/Department of 
      Oncology, Shanghai Medical College, Fudan University/Institute of Pathology, 
      Fudan University, Shanghai 200032, China.
FAU - Ren, M
AU  - Ren M
AD  - Department of Pathology, Fudan University Shanghai Cancer Center/Department of 
      Oncology, Shanghai Medical College, Fudan University/Institute of Pathology, 
      Fudan University, Shanghai 200032, China.
FAU - Cai, X
AU  - Cai X
AD  - Department of Pathology, Fudan University Shanghai Cancer Center/Department of 
      Oncology, Shanghai Medical College, Fudan University/Institute of Pathology, 
      Fudan University, Shanghai 200032, China.
FAU - Lyu, J J
AU  - Lyu JJ
AD  - Department of Pathology, Fudan University Shanghai Cancer Center/Department of 
      Oncology, Shanghai Medical College, Fudan University/Institute of Pathology, 
      Fudan University, Shanghai 200032, China.
FAU - Shen, X X
AU  - Shen XX
AD  - Department of Pathology, Fudan University Shanghai Cancer Center/Department of 
      Oncology, Shanghai Medical College, Fudan University/Institute of Pathology, 
      Fudan University, Shanghai 200032, China.
FAU - Kong, Y Y
AU  - Kong YY
AD  - Department of Pathology, Fudan University Shanghai Cancer Center/Department of 
      Oncology, Shanghai Medical College, Fudan University/Institute of Pathology, 
      Fudan University, Shanghai 200032, China.
LA  - chi
GR  - HYXH2021043/Shanghai Charity Foundation/
GR  - 19MC1911000/Shanghai Science and Technology Development Fund/
GR  - shslczdzk01301/Shanghai Municipal Key Clinical Specialty/
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (PRAME protein, human)
RN  - 0 (Antigens, Neoplasm)
SB  - IM
MH  - Male
MH  - Female
MH  - Humans
MH  - Young Adult
MH  - Adult
MH  - Middle Aged
MH  - Aged
MH  - *Skin Neoplasms/pathology
MH  - Prognosis
MH  - Retrospective Studies
MH  - In Situ Hybridization, Fluorescence
MH  - China
MH  - *Melanoma/diagnosis
MH  - *Nail Diseases/surgery/diagnosis/pathology
MH  - Antigens, Neoplasm
EDAT- 2023/10/08 02:42
MHDA- 2023/11/02 12:42
CRDT- 2023/10/07 22:06
PHST- 2023/11/02 12:42 [medline]
PHST- 2023/10/08 02:42 [pubmed]
PHST- 2023/10/07 22:06 [entrez]
AID - 10.3760/cma.j.cn112151-20230226-00152 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2023 Oct 8;52(10):1006-1011. doi: 
      10.3760/cma.j.cn112151-20230226-00152.