PMID- 37805809
OWN - NLM
STAT- MEDLINE
DCOM- 20231204
LR  - 20231216
IS  - 2470-9239 (Electronic)
IS  - 2470-9239 (Linking)
VI  - 8
IP  - 4
DP  - 2023 Dec
TI  - Diurnal circadian clock gene expression is altered in models of genetic and 
      acquired epilepsy.
PG  - 1523-1531
LID - 10.1002/epi4.12841 [doi]
AB  - OBJECTIVES: Growing evidence demonstrates a relationship between epilepsy and the 
      circadian system. However, relatively little is known about circadian function in 
      disease states, such as epilepsy. This study aimed to characterize brain and 
      peripheral core circadian clock gene expression in rat models of genetic and 
      acquired epilepsy. METHODS: For the Genetic Absence Epilepsy Rats from Strasbourg 
      (GAERS) study, we used 40 GAERS and 40 non-epileptic control (NEC) rats. For the 
      kainic acid status epilepticus (KASE) study, we used 40 KASE and 40 sham rats. 
      Rats were housed in a 7 am:7 pm light-dark cycle. Hypothalamus, hippocampus, 
      liver, and small intestine samples were collected every 3 h throughout the light 
      period. We then assessed core diurnal clock gene expression of per1, cry1, clock, 
      and bmal1. RESULTS: In the GAERS rats, all tissues exhibited significant changes 
      in clock gene expression (P < 0.05) when compared to NEC. In the KASE rats, there 
      were fewer effects of the epileptic condition in the hypothalamus, hippocampus, 
      or small intestine (P > 0.05) compared with shams. SIGNIFICANCE: These results 
      indicate marked diurnal disruption to core circadian clock gene expression in 
      rats with both generalized and focal chronic epilepsy. This could contribute to 
      epileptic symptomology and implicate the circadian system as a viable target for 
      future treatments.
CI  - (c) 2023 The Authors. Epilepsia Open published by Wiley Periodicals LLC on behalf 
      of International League Against Epilepsy.
FAU - Yamakawa, Glenn R
AU  - Yamakawa GR
AD  - Department of Neuroscience, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
FAU - Patel, Meshwa
AU  - Patel M
AD  - Department of Neuroscience, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
FAU - Lin, Runxuan
AU  - Lin R
AUID- ORCID: 0009-0001-8585-2784
AD  - Department of Neuroscience, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
FAU - O'Brien, Terence J
AU  - O'Brien TJ
AD  - Department of Neuroscience, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
AD  - Department of Neurology, The Alfred Hospital, Melbourne, Victoria, Australia.
FAU - Mychasiuk, Richelle
AU  - Mychasiuk R
AD  - Department of Neuroscience, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
FAU - Casillas-Espinosa, Pablo M
AU  - Casillas-Espinosa PM
AUID- ORCID: 0000-0002-6199-9415
AD  - Department of Neuroscience, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
AD  - Department of Neurology, The Alfred Hospital, Melbourne, Victoria, Australia.
LA  - eng
GR  - EP200022/Department of Defense USA Epilepsy Research Program/
GR  - MRF2015957/Department of Health and Aged Care, Australian Government/
GR  - APP1087172/National Health and Medical Research Council/
GR  - APP1091593/National Health and Medical Research Council/
GR  - APP1176426/National Health and Medical Research Council/
PT  - Journal Article
DEP - 20231016
PL  - United States
TA  - Epilepsia Open
JT  - Epilepsia open
JID - 101692036
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Circadian Clocks/genetics
MH  - Circadian Rhythm/genetics
MH  - Brain/metabolism
MH  - *Epilepsy, Absence
MH  - Gene Expression
PMC - PMC10690682
OTO - NOTNLM
OT  - bmal1
OT  - cry1
OT  - per1
OT  - GAERS
OT  - animal model
OT  - circadian
OT  - post-SE
OT  - seizure
OT  - sleep
COIS- We confirm that we have read the Journal's position on issues involved in ethical 
      publication and affirm that this report is consistent with those guidelines. The 
      authors report no competing interests related to the current work.
EDAT- 2023/10/08 06:41
MHDA- 2023/12/04 12:42
PMCR- 2023/10/16
CRDT- 2023/10/08 02:12
PHST- 2023/06/23 00:00 [received]
PHST- 2023/09/12 00:00 [accepted]
PHST- 2023/12/04 12:42 [medline]
PHST- 2023/10/08 06:41 [pubmed]
PHST- 2023/10/08 02:12 [entrez]
PHST- 2023/10/16 00:00 [pmc-release]
AID - EPI412841 [pii]
AID - 10.1002/epi4.12841 [doi]
PST - ppublish
SO  - Epilepsia Open. 2023 Dec;8(4):1523-1531. doi: 10.1002/epi4.12841. Epub 2023 Oct 
      16.