PMID- 37806044
OWN - NLM
STAT- MEDLINE
DCOM- 20231101
LR  - 20231101
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1710
DP  - 2023 Nov 8
TI  - Development and validation of a deep eutectic solvent-assisted liquid-liquid 
      extraction method for simultaneous quantification of six steroid hormones in 
      serum by liquid chromatography-tandem mass spectrometry.
PG  - 464413
LID - S0021-9673(23)00638-6 [pii]
LID - 10.1016/j.chroma.2023.464413 [doi]
AB  - Steroid hormones have been reported to be associated with endocrine system 
      diseases. This paper proposes a novel procedure of deep eutectic solvent 
      (DES)-assisted liquid-liquid extraction (LLE) to extract six steroid hormones 
      (including cortisone, cortisol, androstenedione, testosterone, 
      17-hydroxyprogesterone, and progesterone) from serum coupled with liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS). A total of five types of 
      L-proline, choline chloride, and citric acid-based DESs were tailored; the DES 
      from L-proline and ethylene glycol at a molar ratio of 1:4 with 20 % acetonitrile 
      was selected as the best-fit assisted solvent for the six steroid hormones 
      compared with other DESs. The parameters for extraction by selected DES were 
      optimized using Box-Behnken design (BBD), and the optimal extraction conditions 
      are 200 microL of acetonitrile, 100 microL of the sample, and 80 microL of DES. Under optimum 
      conditions, the method has good linear calibration ranges (between 0.07 ng mL(-1) 
      and 600 ng mL(-1)), correlation coefficients of determination (r(2)>0.99), and 
      low limits of quantification (between 0.02 and 0.60 ng mL(-1)). The extraction 
      recoveries were in the range of 81.84-114.43 %, and the intra-day and inter-day 
      relative standard deviations (RSDs) were less than 10 %.In general, the 
      DES-LC-MS/MS method is a simple and environmentally-friendly method, which can be 
      complementary to the presently available methods for determining steroid hormones 
      in serum.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Li, Jufang
AU  - Li J
AD  - Zhejiang Biozon Medical Technology Co., Ltd, Hangzhou, Zhejiang 310000, China.
FAU - Xu, Aiping
AU  - Xu A
AD  - Laprode (Zhejiang) Analysis Co., Ltd, Hangzhou, Zhejiang 310000, China.
FAU - Xue, Jianyou
AU  - Xue J
AD  - Zhejiang Biozon Medical Technology Co., Ltd, Hangzhou, Zhejiang 310000, China.
FAU - Qian, Wei
AU  - Qian W
AD  - Zhejiang Biozon Medical Technology Co., Ltd, Hangzhou, Zhejiang 310000, China.
FAU - Xu, Ping
AU  - Xu P
AD  - Zhejiang Biozon Medical Technology Co., Ltd, Hangzhou, Zhejiang 310000, China.
FAU - Hu, Zhineng
AU  - Hu Z
AD  - Zhejiang Biozon Medical Technology Co., Ltd, Hangzhou, Zhejiang 310000, China.
FAU - Chen, Chen
AU  - Chen C
AD  - Department of Clinical Laboratory, Women's Hospital of Nanjing Medical 
      University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 
      210004, China. Electronic address: chenchen1986@njmu.edu.cn.
FAU - Wu, Chaochao
AU  - Wu C
AD  - Zhejiang Biozon Medical Technology Co., Ltd, Hangzhou, Zhejiang 310000, China. 
      Electronic address: wuchaochao@biozon.com.cn.
LA  - eng
PT  - Journal Article
DEP - 20230930
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Deep Eutectic Solvents)
RN  - 0 (Steroids)
RN  - WI4X0X7BPJ (Hydrocortisone)
RN  - 0 (Acetonitriles)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Chromatography, Liquid/methods
MH  - *Deep Eutectic Solvents
MH  - *Tandem Mass Spectrometry
MH  - Limit of Detection
MH  - Steroids/analysis
MH  - Liquid-Liquid Extraction
MH  - Hydrocortisone/analysis
MH  - Acetonitriles/analysis
MH  - Proline
MH  - Chromatography, High Pressure Liquid
OTO - NOTNLM
OT  - Deep eutectic solvent
OT  - Liquid chromatography-tandem mass spectrometry
OT  - Liquid-liquid extraction
OT  - Steroid hormones
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/10/09 00:41
MHDA- 2023/11/01 12:44
CRDT- 2023/10/08 18:11
PHST- 2023/07/05 00:00 [received]
PHST- 2023/09/13 00:00 [revised]
PHST- 2023/09/24 00:00 [accepted]
PHST- 2023/11/01 12:44 [medline]
PHST- 2023/10/09 00:41 [pubmed]
PHST- 2023/10/08 18:11 [entrez]
AID - S0021-9673(23)00638-6 [pii]
AID - 10.1016/j.chroma.2023.464413 [doi]
PST - ppublish
SO  - J Chromatogr A. 2023 Nov 8;1710:464413. doi: 10.1016/j.chroma.2023.464413. Epub 
      2023 Sep 30.