PMID- 37806254
OWN - NLM
STAT- MEDLINE
DCOM- 20231101
LR  - 20231105
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 682
DP  - 2023 Nov 19
TI  - SIRT1 Asn346 sugar chain promoting collagen deacetylation protective effect on 
      osteoblasts under stress.
PG  - 148-155
LID - S0006-291X(23)01111-7 [pii]
LID - 10.1016/j.bbrc.2023.09.075 [doi]
AB  - Silencing type information regulator homolog 1 (SIRT1) is a class of nicotinamide 
      adenine dinucleotide (NAD(+)) dependent deacetylases, which is the convergence 
      point of important physiological processes in vivo, namely, osteoblast aging, 
      energy metabolism, and bone remodeling. To verify whether the O-acetylglucosamine 
      (O-GlcNAc) modification of SIRT1 in the nucleus of osteoblasts enhances its 
      deacetylase activity under stress and protects osteoblasts through the RANK/RANKL 
      signaling pathway by collagen deacetylation. The R language and online data 
      research identified SIRT1 as being involved in bone metabolism. Enrichment 
      analysis showed that SIRT1 is involved in osteoblast transcription, apoptosis, 
      and deacetylation pathways. Interactive Immuno-blotting and immunofluorescence 
      experiments revealed that SIRT1 and O-glycosylation catalytic enzyme (OGT) were 
      localized in the nucleus. Mass Spectrometry analysis showed that O-glycosylation 
      occurred on the asparagine at the 346th position of SIRT1, and N346(th) was 
      located in the central domain of SIRT1. Furthermore, the protein structure 
      analysis of PyMol also proved that the OGT binding region was in the central 
      domain of SIRT1. Under physiological conditions, both wtSIRT1 and SIRT1(N346R) 
      can inhibit RANKL-mediated transcriptional activation. The RT-PCR detection 
      results showed that wtSIRT1 reduced RANKL transcription under the conditions of 
      apoptotic agent treatment. The finding that SIRT1 can regulate the physiological 
      process of bone remodeling through the RANK/RANKL signaling pathway in 
      osteoblasts under stress. The O-glycosylation and deacetylation activity of SIRT1 
      significantly increased, regulating the balance between osteoblast survival and 
      apoptosis by deacetylation of key proteins such as RANKL.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Cai, Min
AU  - Cai M
AD  - Department of Geriatric Medicine, Fujian Provincial Hospital, Shengli Clinical 
      Medical College, Fujian Medical University, Fuzhou, Fujian, China.
FAU - Chen, Yaoqi
AU  - Chen Y
AD  - Department of Geriatric Medicine, Fujian Provincial Hospital, Shengli Clinical 
      Medical College, Fujian Medical University, Fuzhou, Fujian, China; Department of 
      Endocrinology, Fujian Provincial Hospital, Shengli Clinical Medical College, 
      Fujian Medical University, Fuzhou, Fujian, China.
FAU - Lin, Yiting
AU  - Lin Y
AD  - Department of Geriatric Medicine, Fujian Provincial Hospital, Shengli Clinical 
      Medical College, Fujian Medical University, Fuzhou, Fujian, China; Department of 
      Endocrinology, Fujian Provincial Hospital, Shengli Clinical Medical College, 
      Fujian Medical University, Fuzhou, Fujian, China.
FAU - Hu, Zhangjie
AU  - Hu Z
AD  - Department of Geriatric Medicine, Fujian Provincial Hospital, Shengli Clinical 
      Medical College, Fujian Medical University, Fuzhou, Fujian, China; Department of 
      Endocrinology, Fujian Provincial Hospital, Shengli Clinical Medical College, 
      Fujian Medical University, Fuzhou, Fujian, China.
FAU - Li, Lizhi
AU  - Li L
AD  - Department of Geriatric Medicine, Fujian Provincial Hospital, Shengli Clinical 
      Medical College, Fujian Medical University, Fuzhou, Fujian, China; Department of 
      Pediatric Surgery, Fujian Provincial Hospital, Shengli Clinical Medical College, 
      Fujian Medical University, Fuzhou, Fujian, China.
FAU - Huang, Huping
AU  - Huang H
AD  - Department of Gastroenterology, Fujian Provincial Hospital, Shengli Clinical 
      Medical College, Fujian Medical University, Fuzhou, Fujian, China. Electronic 
      address: huanghuping@fjmu.edu.cn.
FAU - Lin, Jianli
AU  - Lin J
AD  - Department of Endocrinology, Fujian Provincial Hospital, Shengli Clinical Medical 
      College, Fujian Medical University, Fuzhou, Fujian, China. Electronic address: 
      linjianli@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231004
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - 0 (Sugars)
RN  - 0 (Collagen Type I)
RN  - 0U46U6E8UK (NAD)
SB  - IM
MH  - *Sirtuin 1/genetics/metabolism
MH  - *Sugars/metabolism
MH  - Collagen Type I/metabolism
MH  - NAD/metabolism
MH  - Osteoblasts/metabolism
OTO - NOTNLM
OT  - Bone metabolism
OT  - Deacetylation
OT  - Osteoblast
OT  - Osteoporosis
OT  - SIRT1
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/10/09 00:41
MHDA- 2023/11/01 12:44
CRDT- 2023/10/08 18:18
PHST- 2023/09/04 00:00 [received]
PHST- 2023/09/22 00:00 [revised]
PHST- 2023/09/25 00:00 [accepted]
PHST- 2023/11/01 12:44 [medline]
PHST- 2023/10/09 00:41 [pubmed]
PHST- 2023/10/08 18:18 [entrez]
AID - S0006-291X(23)01111-7 [pii]
AID - 10.1016/j.bbrc.2023.09.075 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2023 Nov 19;682:148-155. doi: 
      10.1016/j.bbrc.2023.09.075. Epub 2023 Oct 4.