PMID- 37807305
OWN - NLM
STAT- MEDLINE
DCOM- 20231010
LR  - 20231010
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 69
IP  - 9
DP  - 2023 Sep 30
TI  - LncRNA MEG3 promotes neuronal apoptosis in rats with ischemic cerebral infarction 
      via the TGF-beta1 pathway.
PG  - 239-244
LID - 10.14715/cmb/2023.69.9.37 [doi]
AB  - To study the influence of long non-coding ribonucleic acid maternally expressed 
      gene 3 (lncRNA MEG3) on the neuronal apoptosis in rats with ischemic cerebral 
      infarction, and to analyze its regulatory effect on the transforming growth 
      factor-beta 1 (TGF-beta1) pathway. A total of 36 Sprague-Dawley rats were randomly 
      assigned into sham group, model group and low expression group. Ischemic cerebral 
      infarction modeling was constructed in rats of the model group and low expression 
      group. Corresponding adenoviruses were intracranially injected in rats of low 
      expression group to knock down lncRNA MEG3 expression. At 24 h after the 
      operation, the neurological function of rats was evaluated in each group, and the 
      expression level of lncRNA MEG3 in cerebral tissues was determined using 
      quantitative polymerase chain reaction (qPCR). The infarct size was measured via 
      2,3,5-triphenyltetrazolium chloride (TTC) staining. The apoptosis level of 
      neurons in cerebral tissues was determined using terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. Besides, 
      enzyme-linked immunosorbent assay (ELISA) was performed to determine the contents 
      of inflammatory factors in cerebral tissues. Expression levels of 
      apoptosis-associated proteins and vital genes in the TGF-beta1 signaling pathway in 
      rat cerebral tissues were measured using Western blotting. Compared with the sham 
      group, rats in the model group exhibited substantial increases in the 
      neurological score and apoptosis level of neurons (p<0.01). Relative levels of 
      lncRNA MEG3, interleukin (IL)-6, tumor necrosis factor-alpha (TNF-alpha), Caspase-3, 
      TGF-beta1, small mothers against decapentaplegic homolog 2 (Smad2) and Smad3 
      (p<0.01) were higher in a model group than those in sham group. Notable declines 
      in the content of IL-10 (p<0.01) and the ratio of B-cell lymphoma 2 (Bcl-2)/Bcl 
      associated X protein (Bax) (p<0.01) were seen in the model group compared with 
      the sham group. The abovementioned changes in the model group were partially 
      abolished in the low expression group. LncRNA MEG3 is upregulated in the cerebral 
      tissues of rats with ischemic cerebral infarction. It induces an inflammatory 
      response, expands cerebral infarct size, and promotes neuronal apoptosis and 
      impairment by activating the TGF-beta1 pathway.
FAU - Long, Shiyu
AU  - Long S
AD  - Department of Neurology, Gaoping District People's Hospital of Nanchong, 
      Nanchong, China. lonshiyu@163.com.
FAU - Chen, Jingli
AU  - Chen J
AD  - Department of Neurology, Gaoping District People's Hospital of Nanchong, 
      Nanchong, China. pcpyp_123@163.com.
FAU - Tan, Shuwei
AU  - Tan S
AD  - Department of Neurology, Gaoping District People's Hospital of Nanchong, 
      Nanchong, China. 824335382@qq.com.
FAU - Zhou, Rong
AU  - Zhou R
AD  - Department of Neurology, Gaoping District People's Hospital of Nanchong, 
      Nanchong, China. zhy6151165@163.com.
FAU - He, Su
AU  - He S
AD  - Department of Neurology, Nanchong Second People's Hospital, Nanchong, China. 
      sophiephya0215@sina.com.
FAU - Jia, Jiedeng
AU  - Jia J
AD  - Dean's office, Gaoping District People's Hospital of Nanchong, Nanchong, China. 
      jiangjd2000@126.com.
LA  - eng
PT  - Journal Article
DEP - 20230930
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (MEG3 non-coding RNA, rat)
SB  - IM
MH  - Animals
MH  - Rats
MH  - *Apoptosis/genetics
MH  - *Cerebral Infarction/genetics/metabolism
MH  - Disease Models, Animal
MH  - Rats, Sprague-Dawley
MH  - *RNA, Long Noncoding/genetics
MH  - Signal Transduction
MH  - Transforming Growth Factor beta1/metabolism
EDAT- 2023/10/09 06:41
MHDA- 2023/10/10 06:42
CRDT- 2023/10/09 00:44
PHST- 2023/07/31 00:00 [received]
PHST- 2023/10/10 06:42 [medline]
PHST- 2023/10/09 06:41 [pubmed]
PHST- 2023/10/09 00:44 [entrez]
AID - 10.14715/cmb/2023.69.9.37 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2023 Sep 30;69(9):239-244. doi: 
      10.14715/cmb/2023.69.9.37.