PMID- 3780973
OWN - NLM
STAT- MEDLINE
DCOM- 19870107
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 208
IP  - 2
DP  - 1986 Nov 24
TI  - Synthesis, processing, and secretion of rat immunoglobulin E made in Xenopus 
      oocytes.
PG  - 369-72
AB  - Rat immunoglobulin E (IgE) synthesized in Xenopus laevis oocytes, injected with 
      rat plasmacytoma mRNA, was analysed by specific immunoprecipitation and 
      SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing 
      conditions. The results indicate that the oocytes will translate and correctly 
      process the rat IgE heavy and light chains, resulting in secretion of a correctly 
      assembled, normal immunoglobulin molecule. The normal, extensive glycosylation of 
      the IgE heavy chain (e-chain) is faithfully carried out by the oocytes; 
      therefore, this posttranslational modification is apparently of an unspecific 
      nature, and does not depend upon a mechanism specific for plasma cells.
FAU - Lund, T
AU  - Lund T
FAU - Bravo, R
AU  - Bravo R
FAU - Johansen, H R
AU  - Johansen HR
FAU - Zeuthen, J
AU  - Zeuthen J
FAU - Vuust, J
AU  - Vuust J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (RNA, Messenger)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Glycosylation
MH  - Immunoglobulin E/*biosynthesis/metabolism
MH  - Microinjections
MH  - Molecular Weight
MH  - Oocytes/metabolism
MH  - Protein Biosynthesis
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Xenopus laevis
EDAT- 1986/11/24 00:00
MHDA- 1986/11/24 00:01
CRDT- 1986/11/24 00:00
PHST- 1986/11/24 00:00 [pubmed]
PHST- 1986/11/24 00:01 [medline]
PHST- 1986/11/24 00:00 [entrez]
AID - 0014-5793(86)81051-1 [pii]
AID - 10.1016/0014-5793(86)81051-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1986 Nov 24;208(2):369-72. doi: 10.1016/0014-5793(86)81051-1.