PMID- 37810669
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 8
IP  - 39
DP  - 2023 Oct 3
TI  - Development of a Lateral-Flow Immunochromatographic Strip for the Detection of 
      Oxytetracycline Residues in Biological Fluids.
PG  - 36237-36244
LID - 10.1021/acsomega.3c04759 [doi]
AB  - Oxytetracycline (OTC) is extensively used in veterinary medicine and for growth 
      promotion around the globe. The indiscriminate use of OTC in food-producing 
      animals leaves residues in animal products. The presence of these residues in 
      animal products causes economic losses and harmful effects on consumers. 
      Different regulatory bodies set maximum residue limits (MRLs) for different 
      tetracyclines. To avoid harmful effects, there is a need for a simple, fast, and 
      economical method for the screening of animal products. In this study, a fast, 
      economical, and user-friendly lateral-flow immunochromatographic (LFIC) assay 
      based on gold nanoparticles (AuNPs) was developed to detect the presence of OTC 
      residues in biological fluids. AuNPs provided visual results as red lines in 6-15 
      min. Polyclonal rabbit IgG antibodies were produced using the immunogen of OTC. 
      These antibodies were purified by the combined ammonium sulfate-octanoic acid 
      precipitation method. Antibodies were conjugated to AuNPs as recognition 
      biomolecules. A LFIC strip was optimized using borate buffer spiked with 
      different concentrations of the OTC. The visual limit of detection (LOD) in 
      different biological samples (milk, serum, and urine) was determined using 
      samples spiked with OTC. The LOD was found to be 15 mug/L, which is very low from 
      the MRL (100 mug/L) set by different regulatory authorities. This LFIC strip can 
      be used to detect OTC residues in biological fluids for point-of-care testing 
      (POCT). These strips are easy to use, cost-effective, and portable and provide 
      quick results without the use of laboratory instruments.
CI  - (c) 2023 The Authors. Published by American Chemical Society.
FAU - Aleem, Abdul
AU  - Aleem A
AUID- ORCID: 0009-0007-9145-3732
AD  - Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad 
      38000, Pakistan.
FAU - Akhtar, Bushra
AU  - Akhtar B
AD  - Department of Pharmacy, University of Agriculture, Faisalabad 38000, Pakistan.
FAU - Muhammad, Faqir
AU  - Muhammad F
AD  - Department of Biosciences, Faculty of Veterinary Science, Bahauddin Zakariya 
      University, Multan 60800, Pakistan.
FAU - Qureshi, Anas Sarwar
AU  - Qureshi AS
AD  - Department of Anatomy, University of Agriculture, Faisalabad 38000, Pakistan.
FAU - Rahman, Sajjad-Ur
AU  - Rahman SU
AD  - Institute of Microbiology, University of Agriculture, Faisalabad 38000, Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20230920
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC10552086
COIS- The authors declare no competing financial interest.
EDAT- 2023/10/09 12:43
MHDA- 2023/10/09 12:44
PMCR- 2023/09/20
CRDT- 2023/10/09 06:07
PHST- 2023/07/03 00:00 [received]
PHST- 2023/09/08 00:00 [accepted]
PHST- 2023/10/09 12:44 [medline]
PHST- 2023/10/09 12:43 [pubmed]
PHST- 2023/10/09 06:07 [entrez]
PHST- 2023/09/20 00:00 [pmc-release]
AID - 10.1021/acsomega.3c04759 [doi]
PST - epublish
SO  - ACS Omega. 2023 Sep 20;8(39):36237-36244. doi: 10.1021/acsomega.3c04759. 
      eCollection 2023 Oct 3.