PMID- 37811950
OWN - NLM
STAT- MEDLINE
DCOM- 20231102
LR  - 20231102
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 199
DP  - 2023 Sep 22
TI  - Immunofluorescent Labeling in Nasal Mucosa Tissue Sections of Allergic Rhinitis 
      Rats via Multicolor Immunoassay.
LID - 10.3791/65937 [doi]
AB  - Allergic rhinitis (AR) is a chronic, non-infectious inflammatory disease of the 
      nasal mucosa, primarily mediated by specific immunoglobulin E (IgE), affecting 
      approximately 10%-20% of the world's population. While immunofluorescence (IF) 
      staining has long been a standard technique for detecting disease-specific 
      protein expression, conventional IF techniques are limited in their ability to 
      detect the expression levels of three or more proteins in the same sample. 
      Consequently, multicolor IF techniques have been developed in recent years, which 
      allow the simultaneous labeling of multiple targets in cells or tissues. This 
      protocol provides a comprehensive overview of the process for establishing a rat 
      model of AR, obtaining nasal mucosal samples, and the technical procedures for 
      multicolor immunofluorescence. All rats in the AR group exhibited typical 
      symptoms such as sneezing, a runny nose, and an itchy nose, with behavioral 
      observations scoring >/=5 points. Hematoxylin and eosin (H&E) staining revealed 
      increased inflammatory cell counts and disrupted nasal mucosal integrity in the 
      AR group. Multicolor immunofluorescence (mIF) demonstrated increased expression 
      of RORgammat and TICAM-1, while Foxp3 expression decreased in the nasal mucosa tissue 
      of AR rats.
FAU - Hu, Yucheng
AU  - Hu Y
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Luo, Hongping
AU  - Luo H
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Li, Junjie
AU  - Li J
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Zhang, Rong
AU  - Zhang R
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Fu, Linyou
AU  - Fu L
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Ren, Qiuyi
AU  - Ren Q
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Chen, Yue
AU  - Chen Y
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Huang, Xueting
AU  - Huang X
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Zhou, Zhihang
AU  - Zhou Z
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Yuan, Hao
AU  - Yuan H
AD  - Hospital of Chengdu University of Traditional Chinese Medicine.
FAU - Tian, Li
AU  - Tian L
AD  - Hospital of Chengdu University of Traditional Chinese Medicine; 
      18980880129@qq.com.
FAU - Wang, Xiaobo
AU  - Wang X
AD  - Meishan Hospital of Chengdu University of Traditional Chinese Medicine; 
      Vitadrwang@cdutcm.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Review
PT  - Video-Audio Media
DEP - 20230922
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Coloring Agents)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Rhinitis, Allergic
MH  - Nasal Mucosa
MH  - Immunoglobulin E
MH  - Coloring Agents
MH  - Disease Models, Animal
MH  - Ovalbumin
EDAT- 2023/10/09 12:42
MHDA- 2023/11/02 12:46
CRDT- 2023/10/09 09:04
PHST- 2023/11/02 12:46 [medline]
PHST- 2023/10/09 12:42 [pubmed]
PHST- 2023/10/09 09:04 [entrez]
AID - 10.3791/65937 [doi]
PST - epublish
SO  - J Vis Exp. 2023 Sep 22;(199). doi: 10.3791/65937.