PMID- 37811976
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231216
IS  - 2165-0497 (Electronic)
IS  - 2165-0497 (Linking)
VI  - 11
IP  - 6
DP  - 2023 Dec 12
TI  - Development and evaluation of a real-time quantitative PCR for the detection of 
      equine infectious anemia virus.
PG  - e0259923
LID - 10.1128/spectrum.02599-23 [doi]
LID - e02599-23
AB  - Equine infectious anemia (EIA) has a worldwide distribution and causes 
      significant losses to the equine industry worldwide. A reliable detection method 
      is necessary to control the transmission of EIA virus (EIAV). Currently, most of 
      the available real-time PCR assays, including the qPCR of recommended by WOAH, 
      are developed according to the sequences of European or American EIAV strains; 
      however, the primers and probe sequences have low homology with Asian EIAV 
      strains. To the best of our knowledge, no qPCR method capable of the well 
      detection of Asian EIAV strains, especially Chinese EIAV strains, has been 
      published to date. The development of a sensitive, specific, and rapid qPCR assay 
      for the detection of the EIAV strains is therefore of great importance.
FAU - Li, Shuaijie
AU  - Li S
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
FAU - Guo, Kui
AU  - Guo K
AUID- ORCID: 0000-0001-6274-1857
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
FAU - Wang, Xuefeng
AU  - Wang X
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
FAU - Lin, Yuezhi
AU  - Lin Y
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
FAU - Wang, Jinhui
AU  - Wang J
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
FAU - Wang, Yaoxin
AU  - Wang Y
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
FAU - Du, Cheng
AU  - Du C
AUID- ORCID: 0000-0002-1686-6189
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
FAU - Hu, Zhe
AU  - Hu Z
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
AD  - WOAH Reference Laboratory for Equine Infectious Anemia, Harbin Veterinary 
      Research Institute of Chinese Academy of Agricultural Sciences , Harbin, China.
FAU - Wang, Xiaojun
AU  - Wang X
AUID- ORCID: 0000-0003-4521-4099
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, the Chinese Academy of Agriculture Sciences , Harbin, China.
AD  - WOAH Reference Laboratory for Equine Infectious Anemia, Harbin Veterinary 
      Research Institute of Chinese Academy of Agricultural Sciences , Harbin, China.
LA  - eng
GR  - 2021YFD1800500/MOST | National Key Research and Development Program of China 
      (NKPs)/
GR  - 2022YFD1800200/MOST | National Key Research and Development Program of China 
      (NKPs)/
PT  - Journal Article
DEP - 20231009
PL  - United States
TA  - Microbiol Spectr
JT  - Microbiology spectrum
JID - 101634614
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Horses
MH  - *Infectious Anemia Virus, Equine/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - *Equine Infectious Anemia/diagnosis
MH  - DNA Primers/genetics
PMC - PMC10715080
OTO - NOTNLM
OT  - equine infectious anemia
OT  - gag gene
OT  - real-time quantitative PCR
OT  - tat gene
COIS- The authors declare no conflicts of interest.
EDAT- 2023/10/09 12:43
MHDA- 2023/12/17 09:44
PMCR- 2023/10/09
CRDT- 2023/10/09 09:12
PHST- 2023/12/17 09:44 [medline]
PHST- 2023/10/09 12:43 [pubmed]
PHST- 2023/10/09 09:12 [entrez]
PHST- 2023/10/09 00:00 [pmc-release]
AID - 02599-23 [pii]
AID - spectrum.02599-23 [pii]
AID - 10.1128/spectrum.02599-23 [doi]
PST - ppublish
SO  - Microbiol Spectr. 2023 Dec 12;11(6):e0259923. doi: 10.1128/spectrum.02599-23. 
      Epub 2023 Oct 9.